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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr308) antibody

Details for Product No. ABIN265198
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Antigen
Synonyms
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AK ... show more
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RAC, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, Rac, PKBalpha, CWS6, PKB-ALPHA, PRKBA, RAC-ALPHA show less
Epitope
pThr308
(133), (86), (64), (60), (58), (47), (36), (33), (29), (19), (18), (17), (17), (17), (16), (14), (10), (10), (9), (8), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(932), (552), (522), (164), (153), (115), (94), (24), (18), (4), (2), (1), (1), (1), (1)
Host
Rabbit
(795), (144), (10), (5), (3), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(33), (27), (26), (22), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (7), (7), (7), (7), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(714), (427), (297), (158), (150), (133), (119), (65), (58), (41), (11), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
Quantity 1 vial
Quantity Comment Contains 10 Blots
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Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN265198
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Immunogen Chemically synthesized phosphopeptide derived from the region of human Akt1 that contains threonine 308.
Isotype IgG
Specificity This antibody detects Akt/PKB pThr308.
Purification Affinity chromatography
Alternative Name AKT1 / PKB
Background Akt, also known as Protein Kinase B (PKB) or RAC- , is a 60 kDa serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3 , caspase 9, BAD, and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins like FAK (when FAK is phosphorylated at tyrosine 397), thereby linking activation to the cytoskeleton. Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at threonine 308 is necessary for phosphorylation of serine 473, which is required for full activation of Akt.
Alternate names: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431.1
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes Western blot: (1: 1000 starting dilution). Positive control: 3T3-L1 pre-adipocytes +/- PDGF treatment, HEK293 cells coexperessingRasV12 and RacL61. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer Phosphate buffered saline, pH 7.3, with 1.0 mg/ml BSA 0.05% sodium azide and 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
Expiry Date 12 months
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr308) antibody anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr308) antibody
Background publications Dudek, Datta, Franke et al.: "Regulation of neuronal survival by the serine-threonine protein kinase Akt." in: Science (New York, N.Y.), Vol. 275, Issue 5300, pp. 661-5, 1997 (PubMed).

Franke, Kaplan, Cantley: "PI3K: downstream AKTion blocks apoptosis." in: Cell, Vol. 88, Issue 4, pp. 435-7, 1997 (PubMed).

Datta, Dudek, Tao et al.: "Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery." in: Cell, Vol. 91, Issue 2, pp. 231-41, 1997 (PubMed).

del Peso, González-García, Page et al.: "Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt." in: Science (New York, N.Y.), Vol. 278, Issue 5338, pp. 687-9, 1997 (PubMed).

Genot, Arrieumerlou, Ku et al.: "The T-cell receptor regulates Akt (protein kinase B) via a pathway involving Rac1 and phosphatidylinositide 3-kinase." in: Molecular and cellular biology, Vol. 20, Issue 15, pp. 5469-78, 2000 (PubMed).

Nelson, Fry: "Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition." in: The Journal of biological chemistry, Vol. 276, Issue 18, pp. 14842-7, 2001 (PubMed).

Persad, Attwell, Gray et al.: "Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 27462-9, 2001 (PubMed).

Hsu, Shi, Krajewski et al.: "The AKT kinase is activated in multiple myeloma tumor cells." in: Blood, Vol. 98, Issue 9, pp. 2853-5, 2001 (PubMed).

Beffert, Morfini, Bock et al.: "Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49958-64, 2002 (PubMed).

Validation Images
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