Connexin 43/GJA1 antibody
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- Target See all Connexin 43/GJA1 (GJA1) Antibodies
- Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))
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Reactivity
- Human, Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Connexin 43/GJA1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects endogenous levels of GJA1 / Cx43 protein(region surrounding Ser369).
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product GJA1 Primary Antibody
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- Application Notes
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ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid Repeated freeze/thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))
- Alternative Name
- GJA1 / Cx43 (GJA1 Products)
- Synonyms
- AVSD3 antibody, CX43 antibody, DFNB38 antibody, GJAL antibody, HLHS1 antibody, HSS antibody, ODDD antibody, Cx43 antibody, AU042049 antibody, AW546267 antibody, Cnx43 antibody, Cx43alpha1 antibody, Gja-1 antibody, Npm1 antibody, connexin43 antibody, cx43a1 antibody, gja1 antibody, zfCx43.3 antibody, connexin 43 antibody, gja1-A antibody, cx43 antibody, gjal antibody, oddd antibody, dfnb38 antibody, gap junction protein alpha 1 antibody, gap junction protein, alpha 1 antibody, connexin 43 antibody, gap junction protein alpha 1 L homeolog antibody, CONNEXIN 43 protein antibody, GJA1 antibody, Gja1 antibody, cx43 antibody, gja1.L antibody, gja1 antibody, CONNEXIN 43 antibody
- Background
- Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
- Molecular Weight
- approx. 43 kDa
- Gene ID
- 2697
- NCBI Accession
- NP_000156
- UniProt
- P17302
- Pathways
- MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
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