DNAJB1 antibody

Catalog No. ABIN265471

Product Details anti-DNAJB1 Antibody

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of HSP40/HDJ-1 protein. (region surrounding Pro303)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Alternative Name: DNAJB1 / HSP40 (DNAJB1 Products)

Synonyms: HSPF1 antibody, Hdj1 antibody, Hsp40 antibody, RSPH16B antibody, Sis1 antibody, CG10578 antibody, DNAJ-1 antibody, DNAJ1 antibody, DROJ1 antibody, Dmel\\CG10578 antibody, DnaJ1 64EF antibody, DroJ1 antibody, EU3500 antibody, HSP40 antibody, HSP40/HDJ1 antibody, Hsp-40 antibody, anon-WO0140519.166 antibody, anon-WO0172774.135 antibody, dHDJ1 antibody, dHDJ1/HSP40 antibody, dHdj1 antibody, dhdJ1 antibody, dhdj-1 antibody, dhdj1 antibody, dnaJ-1 antibody, droj1 antibody, hsp40 antibody, dnajb4 antibody, hdj1 antibody, hspf1 antibody, sis1 antibody, DnaJ-5 antibody, MAS5 antibody, dnaJ antibody, 0610007I11Rik antibody, dnajb1 antibody, zf-Hsp40 antibody, zgc:101068 antibody, DnaJ heat shock protein family (Hsp40) member B1 antibody, DnaJ-like-1 antibody, DnaJ (Hsp40) homolog 5 antibody, type I HSP40 co-chaperone YDJ1 antibody, DnaJ protein antibody, molecular chaperone DnaJ antibody, DnaJ (Hsp40) homolog, subfamily B, member 1a antibody, DNAJB1 antibody, Dnajb1 antibody, DnaJ-1 antibody, dnajb1 antibody, DnaJ-5 antibody, YDJ1 antibody, dnaJ antibody, dnajb1a antibody

Background: Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.Synonyms: DNAJ1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, HDJ-1, HDJ1, HSP-40, HSPF1, Heat shock 40 kDa protein 1

Molecular Weight: approx. 40 kDa

Gene ID: 3337

NCBI Accession: NP_006136

UniProt: P25685