MMP3 antibody

Catalog No. ABIN265531

Product Details anti-MMP3 Antibody

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of MMP-3 protein. (region surrounding Ser453)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MMP3 antibody (ABIN265531)

Wiel, Gras, Vindrieux, Warnier, Gitenay, Le Calvé, Ferrand, Augert, Bernard: "Multidrug resistance protein 3 loss promotes tumor formation by inducing senescence escape." in: Oncogene, Vol. 35, Issue 12, pp. 1596-601, (2016) (PubMed).

Zhang, Lu, Ren, Du, Zheng, Ioannou, Holmgren: "Oxidation of structural cysteine residues in thioredoxin 1 by aromatic arsenicals enhances cancer cell cytotoxicity caused by the inhibition of thioredoxin reductase 1." in: Free radical biology & medicine, Vol. 89, pp. 192-200, (2016) (PubMed).

Muto, Kokubu, Mifune, Inui, Harada, Yoshifumi, Takase, Kuroda, Kurosaka: "Temporary inductions of matrix metalloprotease-3 (MMP-3) expression and cell apoptosis are associated with tendon degeneration or rupture after corticosteroid injection." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 32, Issue 10, pp. 1297-304, (2014) (PubMed).

Ma, Wu, Wang, Wang, Wu, Zhu, Zhao: "Effects of chronic sleep deprivation on the extracellular signal-regulated kinase pathway in the temporomandibular joint of rats." in: PLoS ONE, Vol. 9, Issue 9, pp. e107544, (2014) (PubMed).

Papaconstantinou, Zeglinas, Gazouli, Nastos, Yiallourou, Lykoudis, Evangelou, Papalois, Papaioannou, Vlachogiannakos, Tzathas: "Effect of infliximab on the healing of intestinal anastomosis. An experimental study in rats." in: International journal of surgery (London, England), Vol. 12, Issue 9, pp. 969-75, (2014) (PubMed).

Pasham, Pathare, Fajol, Rexhepaj, Michael, Pakladok, Alesutan, Rotte, Föller, Lang: "OSR1-sensitive small intestinal Na+ transport." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 303, Issue 11, pp. G1212-9, (2012) (PubMed).

Yamakawa, Uchida, Matthay, Makita: "Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 300, Issue 4, pp. L516-25, (2011) (PubMed).

Fu, Chen, Cao, Luo, Qian, Yang, Ren, Su, Cao, Yang, Yan, Shen, Wu, Feng, Wang: "p28GANK overexpression accelerates hepatocellular carcinoma invasiveness and metastasis via phosphoinositol 3-kinase/AKT/hypoxia-inducible factor-1α pathways." in: Hepatology (Baltimore, Md.), Vol. 53, Issue 1, pp. 181-92, (2011) (PubMed).

Zhu, Gao, Qi, Hong, Li, Wang, McHepange, Zhang, Wei, Chen: "Local hyperthermia could induce antiviral activity by endogenous interferon-dependent pathway in condyloma acuminata." in: Antiviral research, Vol. 88, Issue 2, pp. 187-92, (2010) (PubMed).

Target Details for MMP3

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Alternative Name: MMP-3 (MMP3 Products)

Synonyms: CHDS6 antibody, MMP-3 antibody, SL-1 antibody, STMY antibody, STMY1 antibody, STR1 antibody, SLN-1 antibody, SLN1 antibody, STR-1 antibody, Stmy1 antibody, Str1 antibody, MMP10 antibody, chds6 antibody, mmp-3 antibody, mmp13 antibody, mmp3 antibody, sl-1 antibody, stmy antibody, stmy1 antibody, str1 antibody, matrix metallopeptidase 3 antibody, matrix metallopeptidase 3 (stromelysin 1, progelatinase) antibody, matrix metallopeptidase 3 L homeolog antibody, MMP3 antibody, Mmp3 antibody, mmp3.L antibody

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. MMP3 is reported to be implicated in rheumatoid arthritis and some forms of cancer.Synonyms: MMP3, Matrix metalloproteinase-3, SL-1, STMY1, Stromelysin-1, Transin-1

Molecular Weight: approx. 53 kDa

Gene ID: 4314

NCBI Accession: NP_002413

UniProt: P08254