PCNA antibody (Proliferating Cell Nuclear Antigen) (Alexa Fluor 594)

Details for Product anti-PCNA Antibody No. ABIN2656854, Supplier: Log in to see
Antigen
  • 53/13
  • CG9193
  • DmPCNA
  • Dmel\\CG9193
  • MUS209
  • Mus209
  • PCNA
  • PCNA/mus209
  • Pcna
  • dPCNA
  • l(2)02448
  • l(2)56F
  • l(2)56Fa
  • l(2)s1534
  • pcna
  • AO090003000846
  • PCNAR
  • Pcna/cyclin
  • cb16
  • fb36g03
  • wu:fa28e03
  • wu:fb36g03
  • pcna-A
  • mutagen-sensitive 209
  • proliferating cell nuclear antigen
  • Proliferating cell nuclear antigen-like
  • proliferating cell nuclear antigen-like
  • proliferating cell nuclear antigen1
  • mus209
  • pcna
  • pcnA
  • ANI_1_512014
  • AOR_1_1500154
  • PCNA
  • Pcna
  • MGC53867
  • LOC100339381
  • pcna1
Alternatives
anti-Human PCNA antibody for Immunofluorescence (fixed cells)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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304
302
82
72
69
52
52
41
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13
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9
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4
4
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4
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3
3
3
2
1
1
1
1
1
1
Host
Mouse
441
257
15
3
Clonality (Clone)
Monoclonal ()
Conjugate
This PCNA antibody is conjugated to Alexa Fluor 594
31
27
17
16
16
15
11
11
10
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10
10
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10
10
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5
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5
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1
1
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1
1
1
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1
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1
Application
Flow Cytometry (FACS)
550
261
209
204
171
150
108
97
67
62
10
7
7
7
5
4
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Clone PC10
Isotype IgG2a kappa
Cross-Reactivity others
Characteristics The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.
Purification The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Alternative Name PCNA (PCNA Antibody Abstract)
Research Area Cancer, Cell Cycle, Chromatin Binding Proteins, DNA/RNA, Proliferation Markers
Pathways Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C
Supplier Images
Immunofluorescence (IF) image for anti-PCNA antibody (Proliferating Cell Nuclear Antigen)  (Alexa Fluor 594) (ABIN2656854) anti-Proliferating Cell Nuclear Antigen (PCNA) antibody (Alexa Fluor 594)
Background publications Shan, Xu, Zhuo, Morris, Morris: "Induction of p53-dependent activation of the human proliferating cell nuclear antigen gene in chromatin by ionizing radiation." in: The Journal of biological chemistry, Vol. 278, Issue 45, pp. 44009-17, 2003 (PubMed).

Yue, Na, Feng, Ma, Song, Yang: "Expression of p57kip2, Rb protein and PCNA and their relationships with clinicopathology in human pancreatic cancer." in: World journal of gastroenterology : WJG, Vol. 9, Issue 2, pp. 377-80, 2003 (PubMed).

Hoege, Pfander, Moldovan, Pyrowolakis, Jentsch: "RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO." in: Nature, Vol. 419, Issue 6903, pp. 135-41, 2002 (PubMed).

Galkowska, Waldemar, Wojewodzka: "Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes." in: Veterinary immunology and immunopathology, Vol. 53, Issue 3-4, pp. 329-34, 1997 (PubMed).

Yang, Efird, Quintanilla-Martinez, Choi, Harris: "Cell kinetic study of thymic epithelial tumors using PCNA (PC10) and Ki-67 (MIB-1) antibodies." in: Human pathology, Vol. 27, Issue 1, pp. 70-6, 1996 (PubMed).

Wilkins, Harris, Waseem, Lane, Jones: "A study of cell proliferation in formalin-fixed, wax-embedded bone marrow trephine biopsies using the monoclonal antibody PC10, reactive with proliferating cell nuclear antigen (PCNA)." in: The Journal of pathology, Vol. 166, Issue 1, pp. 45-52, 1992 (PubMed).

Hall, Levison, Woods, Yu, Kellock, Watkins, Barnes, Gillett, Camplejohn, Dover: "Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms." in: The Journal of pathology, Vol. 162, Issue 4, pp. 285-94, 1991 (PubMed).

Landberg, Roos: "Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis." in: Cancer research, Vol. 51, Issue 17, pp. 4570-4, 1991 (PubMed).

Woods, Hall, Shepherd, Hanby, Waseem, Lane, Levison et al.: "The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric ..." in: Histopathology, Vol. 19, Issue 1, pp. 21-7, 1991 (PubMed).

Yu, Hall, Fletcher, Camplejohn, Waseem, Lane, Levison: "Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA)." in: Histopathology, Vol. 19, Issue 1, pp. 29-33, 1991 (PubMed).

Landberg, Tan, Roos: "Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle." in: Experimental cell research, Vol. 187, Issue 1, pp. 111-8, 1990 (PubMed).

Travali, Ku, Rizzo, Ottavio, Baserga, Calabretta: "Structure of the human gene for the proliferating cell nuclear antigen." in: The Journal of biological chemistry, Vol. 264, Issue 13, pp. 7466-72, 1989 (PubMed).

Garcia, Coltrera, Gown: "Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis." in: The American journal of pathology, Vol. 134, Issue 4, pp. 733-9, 1989 (PubMed).

Ogata, Ogata, Nakamura, Tan: "Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 4, pp. 2623-7, 1985 (PubMed).