NFATC1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1) (AA 197-304) (Alexa Fluor 488)

Details for Product anti-NFATC1 Antibody No. ABIN2657092, Supplier: Log in to see
Antigen
  • nf-atc
  • nfat2
  • nfatc
  • si:ch211-198c22.2
  • NFATC1
  • 2210017P03Rik
  • AI449492
  • AV076380
  • NF-ATc
  • NFAT2
  • NFATc
  • Nfatcb
  • NF-ATC
  • NF-ATc1
  • NFAT
  • NFATmac
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
  • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1
  • NFATC1
  • nfatc1
  • Nfatc1
Alternatives
anti-Human NFATC1 antibody for Immunofluorescence
Epitope
AA 197-304
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This NFATC1 antibody is conjugated to Alexa Fluor 488
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Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS)
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Options
Supplier
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Clone 7A6
Isotype IgG1
Purification The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Alternative Name NFATc1 (NFATC1 Antibody Abstract)
Background The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3, and NFAT4. All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response. NFAT proteins are predominantly expressed in cells of the immune system but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells. In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
Research Area Signaling
Pathways RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C
Supplier Images
Flow Cytometry (FACS) image for anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 197-304) antibody (Alexa Fluor 488) (ABIN2657092) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 197-304) antibody (Alexa Fluor 488)
Background publications Zhao, Wang, Liu, He, Jia: "NFATc1: functions in osteoclasts." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 5, pp. 576-9, 2010 (PubMed).

Brandt, Liman, Bendfeldt, Mueller, Reinke, Radbruch, Worm, Baumgrass: "Whole blood flow cytometric measurement of NFATc1 and IL-2 expression to analyze cyclosporine A-mediated effects in T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 77, Issue 7, pp. 607-13, 2010 (PubMed).

Serfling, Berberich-Siebelt, Avots: "NFAT in lymphocytes: a factor for all events?" in: Science's STKE : signal transduction knowledge environment, Vol. 2007, Issue 398, pp. pe42, 2007 (PubMed).

Hogan, Chen, Nardone, Rao: "Transcriptional regulation by calcium, calcineurin, and NFAT." in: Genes & development, Vol. 17, Issue 18, pp. 2205-32, 2003 (PubMed).

Timmerman, Healy, Ho, Chen, Goodnow, Crabtree: "Redundant expression but selective utilization of nuclear factor of activated T cells family members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2735-40, 1997 (PubMed).

Hoey, Sun, Williamson, Xu: "Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins." in: Immunity, Vol. 2, Issue 5, pp. 461-72, 1995 (PubMed).

Northrop, Ullman, Crabtree: "Characterization of the nuclear and cytoplasmic components of the lymphoid-specific nuclear factor of activated T cells (NF-AT) complex." in: The Journal of biological chemistry, Vol. 268, Issue 4, pp. 2917-23, 1993 (PubMed).

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