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Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 796-812) antibody

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AA 796-812
(9), (8), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
(82), (41), (30), (5), (5), (3), (2), (2), (1)
(66), (16)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS)
(66), (22), (21), (20), (11), (11), (2), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Quantity 0.1 mg
Shipping to United States ( )
Immunogen The antibody was raised against two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein.
Clone 39C534-1
Isotype IgG1
Specificity In Jurkat, a 97 kDa band is observed.
Purification Protein G purified
Alternative Name EIF4G2 / DAP5 (EIF4G2 Antibody Abstract)
Background Apoptosis, or programmed cell death, occurs during normal cellular differentiation anddevelopment of multicellular organisms. Recently, five novel apoptotic genes (namedDAP genes for: Death Associated Proteins) have been identified that code for proteins,which display a diverse spectrum of biochemical activities. These include a novel type ofcalcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain(DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmicprotein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5)(1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of thetranslation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 wascloned from HeLa cells using a functional approach in attempt to isolate cDNAfragments, which convey resistance to gamma interferon (IFN-g)-induced programmedcell death (3). A fragment of the cDNA when expressed as a mini-protein of 28 kDaprotects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for aprotein of approximate molecular weight of 97 kDa. Low level of expression protects thecells, whereas high level of expression results in cell death (3-4). Alternate Names: anti-Death associated protein 5 antibody, anti-Eukaryotic translation initiation factor 4gamma 2 antibody, anti-Nat1 antibody, anti-Novel APOBEC-1 target 1 antibody,anti-Translation repressor NAT1 antibody.
Gene Symbol: EIF4G2
Gene ID 1982, 13690
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.2 % gelatin, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 796-812) antibody (ABIN265943) anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 796-812) antibody
Product cited in: Escobar, Frank, Suryawan et al.: "Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 6, pp. E1615-21, 2007 (PubMed).

Background publications Wittke, Maedge, Wiedemeyer et al.: "DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells." in: Cancer letters, Vol. 162, Issue 2, pp. 237-43, 2001 (PubMed).

Berger, Brigl, Herrmann et al.: "The apoptosis mediator mDAP-3 is a novel member of a conserved family of mitochondrial proteins." in: Journal of cell science, Vol. 113 ( Pt 20), pp. 3603-12, 2000 (PubMed).

Kimchi: "DAP kinase and DAP-3: novel positive mediators of apoptosis." in: Annals of the rheumatic diseases, Vol. 58 Suppl 1, pp. I14-9, 2000 (PubMed).

Levy-Strumpf, Kimchi: "Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions." in: Oncogene, Vol. 17, Issue 25, pp. 3331-40, 1999 (PubMed).

Levy-Strumpf, Deiss, Berissi et al.: "DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death." in: Molecular and cellular biology, Vol. 17, Issue 3, pp. 1615-25, 1997 (PubMed).

Catalog No. ABIN265943

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