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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) antibody
|Synonyms||p97, AAG1, DAP5, NAT1, FLJ41344, EIF4G2, NAT1B, eif4g2, hm:zeh1307, wu:fa14h01, wu:fb44h01, wu:fb59c06, wu:fc21b05, DKFZp459D102|
Alternatives Western Blotting (WB)
|6 references available|
|Quantity||50 µg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Alternative name||EIF4G2 / DAP5|
|Gene ID||1982, 13690|
|Immunogen||The antibody was raised against two synthetic peptides corresponding to amino acids796-812 of human DAP-5 protein.|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||Apoptosis, or programmed cell death, occurs during normal cellular differentiation anddevelopment of multicellular organisms. Recently, five novel apoptotic genes (namedDAP genes for: Death Associated Proteins) have been identified that code for proteins,which display a diverse spectrum of biochemical activities. These include a novel type ofcalcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain(DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmicprotein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5)(1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of thetranslation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 wascloned from HeLa cells using a functional approach in attempt to isolate cDNAfragments, which convey resistance to gamma interferon (IFN-g)-induced programmedcell death (3). A fragment of the cDNA when expressed as a mini-protein of 28 kDaprotects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for aprotein of approximate molecular weight of 97 kDa. Low level of expression protects thecells, whereas high level of expression results in cell death (3-4). Alternate Names: anti-Death associated protein 5 antibody, anti-Eukaryotic translation initiation factor 4gamma 2 antibody, anti-Nat1 antibody, anti-Novel APOBEC-1 target 1 antibody,anti-Translation repressor NAT1 antibody.|
|Specificity||In Jurkat, a 97 kDa band is observed. Species Reactivity: Cross-reacts with Human and Mouse. Predicted to cross-react with Rat,Bovine, Canineand Monkey proteins based on 100% identity of the immunogen.|
|Application Notes||Suggested working dilutions: Western Blot 1-2 ug/ml. Positive Controls: Jurkat|
|Purification||Aff - Purified|
|Buffer||PBS containing 0.2% gelatin. Preservative: Sodium Azide|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Restrictions||For Research Use only|
|anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) antibody|
Levy-Strumpf, Deiss, Berissi et al.: "DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death." in: Molecular and cellular biology, Vol. 17, Issue 3, pp. 1615-25, 1997 (PubMed).
Levy-Strumpf, Kimchi: "Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions." in: Oncogene, Vol. 17, Issue 25, pp. 3331-40, 1999 (PubMed).
Kimchi: "DAP kinase and DAP-3: novel positive mediators of apoptosis." in: Annals of the rheumatic diseases, Vol. 58 Suppl 1, pp. I14-9, 2000 (PubMed).
Berger, Brigl, Herrmann et al.: "The apoptosis mediator mDAP-3 is a novel member of a conserved family of mitochondrial proteins." in: Journal of cell science, Vol. 113 ( Pt 20), pp. 3603-12, 2000 (PubMed).
Wittke, Maedge, Wiedemeyer et al.: "DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells." in: Cancer letters, Vol. 162, Issue 2, pp. 237-43, 2001 (PubMed).
Escobar, Frank, Suryawan et al.: "Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 6, pp. E1615-21, 2007 (PubMed).
|Hosts||Rabbit (32), Mouse (6)|
|Reactivities||Human (35), Mouse (Murine) (22), Rat (Rattus) (21), Cow (Bovine) (4), Chicken (3), Dog (Canine) (2), Rabbit (2), Cat (Feline) (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (23), Immunofluorescence (IF) (17), ELISA (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||AA 796-812 (3), N-Term (2), AA 805 (1), C-Term (1), pSer194 (1)|