Thymic Stromal Lymphopoietin (TSLP) (AA 29-159) antibody (Biotin)

Details for Product No. ABIN2660798, Supplier: Log in to see
Antigen
  • TSLP
  • thymic stromal lymphopoietin
  • uncharacterized LOC617637
  • TSLP
  • Tslp
  • LOC617637
Epitope
AA 29-159
22
18
11
6
5
5
3
2
2
1
1
1
1
1
Reactivity
Human
147
49
19
2
2
1
1
Host
Goat
107
64
20
1
Clonality (Clone)
Polyclonal ()
Conjugate
Biotin
17
7
6
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
Application
ELISA (Detection)
174
66
62
37
8
5
4
3
3
3
3
2
2
2
2
2
1
1
1
Options
Supplier
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Immunogen AA 29-159 (Accession# AF338732), was expressed in E.coli.
Clone Poly5159
Isotype Ig Fraction, IgG
Cross-Reactivity (Details) Not cross-reactive with recombinant mouse TSLP.
Purification The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Alternative Name TSLP (TSLP Antibody Abstract)
Background TSLP was initially identified in conditioned medium from a mouse thymic stromal cell line. Human and mouse TSLP are only 43 % identical at the amino acid level, and TSLP, like IL-7, exhibits species-specific functions and may function differently in humans and mice. TSLP binds to a heterodimeric receptor constituted by IL-7Ra and TSLPR. TSLPR has low affinity for TSLP, but in combination with IL-7Ra generates a high affinity binding site. TSLP has been associated with the development of allergic disorders in humans, such as asthma, atopic dermatitis, and food allergies. The susceptibility to allergic diseases has been associated to basophil induction by TSLP, and Th2 cytokine production induced by TSLP in mast cells. In addition, TSLP has been associated to the development of Tregs, and blocking TSLPR leads to delayed functional maturation of thymic Tregs. TSLPR has been detected in Tregs from allergic asthmatic patients. TSLP directly activates STAT5 in Tregs and suppresses its suppressive activities, additionally, it inhibits IL-10 production. Human TSLP induces the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes and enhances the T cell stimulatory capacity of the CD11c+ subset of dendritic cells. TSLP induces proliferation of naive T cells. In addition, it can drive Th2 differentiation via the induction of IL-4 production, and drives Th2 cytokine-mediated inflammation by acting on bone marrow-resident progenitors, which promotes basophil hematopoiesis. TSLP is upregulated in epithelial cells by IL-1α, TNFα, and Th2 cytokines.
Research Area Immunology, Innate Immunity, Cytokines
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 1 % BSA and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Images
ELISA image for anti-Thymic Stromal Lymphopoietin (TSLP) (AA 29-159) antibody (Biotin) (ABIN2660798) anti-Thymic Stromal Lymphopoietin (TSLP) (AA 29-159) antibody (Biotin)
Background publications Siracusa, Saenz, Hill, Kim, Headley, Doering, Wherry, Jessup, Siegel, Kambayashi, Dudek, Kubo, Cianferoni, Spergel, Ziegler, Comeau, Artis: "TSLP promotes interleukin-3-independent basophil haematopoiesis and type 2 inflammation." in: Nature, Vol. 477, Issue 7363, pp. 229-33, 2011 (PubMed).

Nguyen, Vanichsarn, Nadeau: "TSLP directly impairs pulmonary Treg function: association with aberrant tolerogenic immunity in asthmatic airway." in: Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology, Vol. 6, Issue 1, pp. 4, 2010 (PubMed).

Mazzucchelli, Hixon, Spolski, Chen, Li, Hall, Willette-Brown, Hurwitz, Leonard, Durum: "Development of regulatory T cells requires IL-7Ralpha stimulation by IL-7 or TSLP." in: Blood, Vol. 112, Issue 8, pp. 3283-92, 2008 (PubMed).

Omori, Ziegler: "Induction of IL-4 expression in CD4(+) T cells by thymic stromal lymphopoietin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 3, pp. 1396-404, 2007 (PubMed).

Rochman, Watanabe, Arima, Liu, Leonard: "Cutting edge: direct action of thymic stromal lymphopoietin on activated human CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 11, pp. 6720-4, 2007 (PubMed).

Reche, Soumelis, Gorman, Clifford, Liu Mr, Travis, Zurawski, Johnston, Liu, Spits, de Waal Malefyt, Kastelein, Bazan: "Human thymic stromal lymphopoietin preferentially stimulates myeloid cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 336-43, 2001 (PubMed).

Levin, Koelling, Friend, Isaksen, Ziegler, Perlmutter, Farr: "Thymic stromal lymphopoietin: a cytokine that promotes the development of IgM+ B cells in vitro and signals via a novel mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 2, pp. 677-83, 1999 (PubMed).