Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
Purification
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
CD179a is an 18 kD Ig V-like protein expressed on pro-B and early pre-B cells. It is a member of the Ig gene superfamily, known as VpreB, VPREB1, and IGVPB. CD179a noncovalently associates with CD179b (λ5) to form a surrogate light (SL) chain. The SL chain is complexed with membrane bound IgM heavy (μH) chain and CD79a/CD79b heterodimer to form the pre-B-cell receptor (PreBCR) complex. The function of CD179a/CD179b is still under investigation though it has been reported that the pre-BCR complex plays a critical role in B cell development.