Interleukin 22 (IL22) (Ala34) antibody

Details for Product No. ABIN2665496, Supplier: Log in to see
Antigen
  • IL-17D
  • IL-22
  • IL-27
  • IL27
  • IL-21
  • IL-D110
  • IL-TIF
  • ILTIF
  • TIFIL-23
  • TIFa
  • zcyto18
  • IL-22a
  • ILTIFa
  • Iltif
  • RGD1561292
  • interleukin-22
  • interleukin 22
  • interleukin 17D
  • il22
  • IL17D
  • IL22
  • Il22
  • LOC100348980
  • IL-22
Epitope
Ala34
44
13
12
9
8
5
3
3
3
3
1
1
1
1
1
Reactivity
Human
218
118
32
2
2
2
2
1
1
1
1
1
Host
Goat
130
106
50
13
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
32
17
12
10
10
10
9
8
8
7
6
6
6
6
6
6
6
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
219
92
62
31
19
13
11
8
6
5
3
3
2
2
1
1
Options
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Immunogen Ala34-Isoleucin179 (Accession# NM_020525) was expressed in E. coli.
Clone Poly5161
Isotype Ig Fraction, IgG
Cross-Reactivity (Details) In sandwich ELISA, less than 10 % cross-reactivity with recombinant mouse IL-22.
Purification The antibody was purified by antigen affinity chromatography.
Alternative Name IL-22 (IL22 Antibody Abstract)
Background IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79 % amino acid identity with murine IL-22 and 25 % identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Images
ELISA image for anti-Interleukin 22 (IL22) (Ala34) antibody (ABIN2665496) anti-Interleukin 22 (IL22) (Ala34) antibody
Background publications Trifari, Kaplan, Tran, Crellin, Spits: "Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells." in: Nature immunology, Vol. 10, Issue 8, pp. 864-71, 2009 (PubMed).

Gu, Yang, Ouyang, Liu, Li, Yang, Bromberg, Chen, Mayer, Unkeless, Xiong: "Interleukin 10 suppresses Th17 cytokines secreted by macrophages and T cells." in: European journal of immunology, Vol. 38, Issue 7, pp. 1807-13, 2008 (PubMed).

Pène, Chevalier, Preisser, Vénéreau, Guilleux, Ghannam, Molès, Danger, Ravon, Lesaux, Yssel, Gascan: "Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7423-30, 2008 (PubMed).

Kebir, Kreymborg, Ifergan, Dodelet-Devillers, Cayrol, Bernard, Giuliani, Arbour, Becher, Prat: "Human TH17 lymphocytes promote blood-brain barrier disruption and central nervous system inflammation." in: Nature medicine, Vol. 13, Issue 10, pp. 1173-5, 2007 (PubMed).

Chung, Yang, Chang, Ma, Tian, Dong: "Expression and regulation of IL-22 in the IL-17-producing CD4+ T lymphocytes." in: Cell research, Vol. 16, Issue 11, pp. 902-7, 2006 (PubMed).

Nagalakshmi, Rascle, Zurawski, Menon, de Waal Malefyt: "Interleukin-22 activates STAT3 and induces IL-10 by colon epithelial cells." in: International immunopharmacology, Vol. 4, Issue 5, pp. 679-91, 2004 (PubMed).

Kotenko, Izotova, Mirochnitchenko, Esterova, Dickensheets, Donnelly, Pestka: "Identification, cloning, and characterization of a novel soluble receptor that binds IL-22 and neutralizes its activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7096-103, 2001 (PubMed).

Dumoutier, Louahed, Renauld: "Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1814-9, 2000 (PubMed).