LCP2 antibody (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa)) (AA 216-434)

Details for Product anti-LCP2 Antibody No. ABIN2666114, Supplier: Log in to see
Antigen
  • AI323664
  • BB161688
  • SLP-76
  • SLP76
  • twm
  • Slp76
  • lymphocyte cytosolic protein 2
  • lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
  • Lcp2
  • LCP2
Alternatives
anti-Human LCP2 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 216-434
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17
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2
2
1
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Reactivity
Human, Mouse (Murine)
153
94
54
32
2
2
1
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Host
Mouse
82
57
19
Clonality (Clone)
Monoclonal ()
Conjugate
This LCP2 antibody is un-conjugated
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4
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1
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1
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Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS)
131
46
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36
15
13
6
6
4
2
1
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Options
Supplier
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Immunogen AA 216-434 of human SLP-76,216-434 of human SLP-76
Clone H76
Isotype IgG2a kappa
Purification The antibody was purified by affinity chromatography.
Alternative Name SLP-76 (LCP2 Antibody Abstract)
Background SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind, this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.
Research Area Signaling
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Upon receipt, store frozen at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 216-434) antibody (ABIN2666114) anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 216-434) antibody
Western Blotting (WB) image for anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 216-434) antibody (ABIN2666114) anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 216-434) antibody (Image 2)
Background publications DeFord-Watts, Dougall, Belkaya, Johnson, Eitson, Roybal, Barylko, Albanesi, Wülfing, van Oers: "The CD3 zeta subunit contains a phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 12, pp. 6839-47, 2011 (PubMed).

Tuosto, Michel, Acuto: "p95vav associates with tyrosine-phosphorylated SLP-76 in antigen-stimulated T cells." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1161-6, 1997 (PubMed).

Motto, Ross, Wu, Hendricks-Taylor, Koretzky: "Implication of the GRB2-associated phosphoprotein SLP-76 in T cell receptor-mediated interleukin 2 production." in: The Journal of experimental medicine, Vol. 183, Issue 4, pp. 1937-43, 1996 (PubMed).

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