IL-15 Protein (AA 49-162)
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- Target See all IL-15 (IL15) Proteins
- IL-15 (IL15) (Interleukin 15 (IL15))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 49-162
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Flow Cytometry (FACS)
- Purity
- >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Endotoxin Level
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Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product IL15 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized, carrier-free.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored at -20°C or -70°C.
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- Target
- IL-15 (IL15) (Interleukin 15 (IL15))
- Alternative Name
- IL-15 (IL15 Products)
- Synonyms
- IL15 Protein, AI503618 Protein, IL-15 Protein, interleukin 15 Protein, IL15 Protein, Il15 Protein
- Background
- IL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2. IL-15 is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2, for example, stimulating the proliferation of activated T cells, NK cells and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, activates human neutrophils and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells, such recognition maintains cell survival and intermittent proliferation.
- Molecular Weight
- The 115 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 12.9 kDa. The predicted N-terminal amino acid is Met.
- Pathways
- JAK-STAT Signaling, Glycosaminoglycan Metabolic Process
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