CAS9 antibody

Details for Product No. ABIN2667644, Supplier: Log in to see
Antigen
Reactivity
Streptococcus pyogenes (S. pyogenes)
8
1
Host
Mouse
5
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
9
4
3
3
3
1
1
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Clone 7A9-3A3
Isotype IgG1 kappa
Purification Protein G Chromatography
Background Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
Molecular Weight 160 kDa
Application Notes Validated Applications: IP: 5 μg per IP ICC/IF: 0.5 - 2 μg/mL dilution WB: 0.1 - 1 μg/mL dilution
Restrictions For Research Use only
Concentration 1 μg/μL
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage. This product is guaranteed for 6 months from date of arrival.

Storage -20 °C
Storage Comment Antibodies in solution can be stored at -20°C for 2 years.
Expiry Date 6 months