MLL/KMT2A antibody (AA 2829-2883)

Catalog No. ABIN2668494

Product Details anti-MLL/KMT2A Antibody

Target: MLL/KMT2A (MLL) (Myeloid/lymphoid Or Mixed-Lineage Leukemia (MLL))

Binding Specificity: AA 2829-2883

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLL/KMT2A antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Immunofluorescence (IF)

Purification: Protein A Chromatography

Immunogen: This MLL / HRX antibody was raised against a recombinant protein corresponding to amino acids 2829-2883 of human MLL.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-MLL/KMT2A antibody (ABIN2668494)

Kühn, Löscher, Marschalek: "The IRX1/HOXA connection: insights into a novel t(4;11)- specific cancer mechanism." in: Oncotarget, Vol. 7, Issue 23, pp. 35341-52, (2016) (PubMed).

Kang, Piao, Ryu, Kang, Chang, Keum, Hyun: "Interaction of DNA demethylase and histone methyltransferase upregulates Nrf2 in 5-fluorouracil-resistant colon cancer cells." in: Oncotarget, Vol. 7, Issue 26, pp. 40594-40620, (2016) (PubMed).

Ahmad, Katryniok, Scholz, Merkens, Löscher, Marschalek, Steinhilber: "Inhibition of class I HDACs abrogates the dominant effect of MLL-AF4 by activation of wild-type MLL." in: Oncogenesis, Vol. 3, pp. e127, (2014) (PubMed).

Pradeepa, Grimes, Taylor, Sutherland, Bickmore: "Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins." in: Nucleic acids research, Vol. 42, Issue 14, pp. 9021-32, (2014) (PubMed).

Wilkinson, Ballabio, Geng, North, Tapia, Kerry, Biswas, Roeder, Allis, Melnick, de Bruijn, Milne: "RUNX1 is a key target in t(4;11) leukemias that contributes to gene activation through an AF4-MLL complex interaction." in: Cell reports, Vol. 3, Issue 1, pp. 116-27, (2013) (PubMed).

Target Details for MLL/KMT2A

Target: MLL/KMT2A (MLL) (Myeloid/lymphoid Or Mixed-Lineage Leukemia (MLL))

Alternative Name: MLL / HRX (MLL Products)

Molecular Weight: 180 kDa

Gene ID: 4297

Pathways: Warburg Effect