p300 antibody

Catalog No. ABIN2668569

Product Details anti-p300 Antibody

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Purification: Protein A Chromatography

Immunogen: The antibody was raised against full-length p300 purified from human 293 cells.

Clone: NM11

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-p300 antibody (ABIN2668569)

Dornan, Eckert, Wallace, Shimizu, Ramsay, Hupp, Ball: "Interferon regulatory factor 1 binding to p300 stimulates DNA-dependent acetylation of p53." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 10083-98, (2004) (PubMed).

Bernat, Avvakumov, Mymryk, Banks: "Interaction between the HPV E7 oncoprotein and the transcriptional coactivator p300." in: Oncogene, Vol. 22, Issue 39, pp. 7871-81, (2003) (PubMed).

Dallas, Yaciuk, Moran: "Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes." in: Journal of virology, Vol. 71, Issue 2, pp. 1726-31, (1997) (PubMed).

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: p300 (EP300 Products)

Synonyms: KAT3B antibody, RSTS2 antibody, p300 antibody, A430090G16 antibody, A730011L11 antibody, p300 HAT antibody, E1A binding protein p300 antibody, histone acetyltransferase p300 antibody, EP300 antibody, LOC446056 antibody, ep300 antibody, Ep300 antibody

Molecular Weight: 300 kDa

Gene ID: 2033

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size