EPOR antibody (Erythropoietin Receptor)

Details for Product anti-EPOR Antibody No. ABIN266926, Supplier: Log in to see
Antigen
  • EPOR
  • epor
  • EPO-R
  • erythropoietin receptor
  • Erythropoietin receptor
  • erythropoietin receptor homologue
  • erythropoietin receptor-like
  • EPOR
  • epor
  • Epor
  • LOC100355350
Alternatives
anti-Human EPOR antibody for Enzyme Immunoassay
Reactivity
Human
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Host
Sheep
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1
Clonality
Polyclonal
Conjugate
This EPOR antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Immunogen The immunogen used was the cDNA of the extracellular domain of human EpoR expressed as a protein in E. coli.
Specificity Antibody titers and specificity were analyzed by ELISA assay using control samples by varying concentration of EpoR
Purification Protein G purified
Alternative Name Erythropoietin receptor (EPOR Antibody Abstract)
Background Gene Symbol: EPOR
Gene ID 2057
Pathways JAK-STAT Signaling
Application Notes Western Blot 1:1000, ELISA 1:20, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10-1:500ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry Affinity purified antibody was found to detect EpoR at concentrations of 50 ng/mL. Neutralization of ligand binding has been detected using erythroleukemia cell line TF-1 with 3000 Epo receptors/cell to block Iodine 125 labeled erythropoietin ligand binding. This antibody will recognize EpoR at its migration of 49 kD on SDS-PAGE. High quality secondary anti-sheep reagents are essential for this assay. Past experiments recommend the use of rabbit anti-sheep rather than donkey anti-sheep antibodies as a secondary labeling reagents.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.32 mg/mL
Buffer 0.15M PBS ( pH 7.4)
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Product cited in: Fairchild Benyo, Conrad: "Expression of the erythropoietin receptor by trophoblast cellsin the human placenta." in: Biology of reproduction, Vol. 60, Issue 4, pp. 861-70, 1999 (PubMed).

Harris, Mitchell, Winkelmann: "Ligand binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli." in: The Journal of biological chemistry, Vol. 267, Issue 21, pp. 15205-9, 1992 (PubMed).

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