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HPGD antibody (Hydroxyprostaglandin Dehydrogenase 15-(NAD))

Details for Product anti-HPGD Antibody No. ABIN268223, Supplier: Login to see New
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Synonyms MGC80546, MGC79454, 15-PGDH, AV026552, PGDH, PGDH1, PHOAR1, SDR36C1
Human, Mouse (Murine)
(113), (24), (18), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
(66), (31), (22)
This HPGD antibody is un-conjugated
(5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(80), (43), (28), (24), (17), (11), (10), (7), (5), (4), (3), (1), (1), (1)
Pubmed 4 references available
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Quantity 25 μL
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Immunogen Purified type I human placental 15-PGDH protein
Purification Protein A purified
Alternative Name HPGD (HPGD Antibody Abstract)
Background 15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is a novel tumor suppressor in theCOX-2 pathway. The protein is found in many normal tissues and appears to bedown-regulated in colorectal and lung carcinoma tissue. Alternate Names: anti-Prostaglandin dehydrogenase 1 antibody, anti-PGDH antibody, anti-HPGDantibody, anti-PGDH1 antibody, anti-15 PGDH antibody, anti-15-hydroxy prostaglandindehydrogenase antibody, anti-15-hydroxyprostaglandin dehydrogenase [NAD+]antibody.
Gene Symbol: HPGD
Gene ID 3248
UniProt P15428
Application Notes This 15-PGDH antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. In Western blot bands may be seen at 29 kDa and 58 kDa (homodimer) depending on the lysate used. In ICC/IF cytoplasmic staining was observed in HeLa cells.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry 1:200, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:200, Western Blot 1:5000-1:6000
Protocol Western Blot Protocols specific for 15-PGDH Antibody Western Blot I (LoVo lysates)
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 20ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1 hour.
. Dilute the rabbit anti-15-PGDH primary antibody in blocking buffer and incubate overnight at 4C.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Amersham's ECL is the standard reagent used).**Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Western Blot II (A549 lysates)
. Cell lysates are prepared in RIPA buffer (50 mM 50 mM Tris-HCL/1 % Nonidet P-40/0.25 % Na-deoxycholate/150 mM NaCl/1 mM EDTA/1 mM PMSF) supplemented with a protease inhibitor mixture (Roche Applied Sciences).
. They are then separated on 10 % or 12 % SDS/PAGE (30-150 µg per lane) and transferred to a Immobilon PVDF membrane (Millipore).
. The membrane is blocked with 5 % NFDM in TBS-T (TBS + 0.1 % Tween-20).
. The membrane is then probed with the diluted anti-PGDH antibody , diluted in blocking buffer at RT for 1 hour.
. The membrane is washed 3 times with TBS-T.
. The membrane is then incubated with a biotinylated goat anti-rabbit IgG (diluted as per manufacturer's guidelines in blocking buffer) at RT for 1 hour.
. The membrane was washed extensively.
. The membrane is then incubated with an HRP-conjugated streptavidin (1:2,000) complex at RT for 1 hour.
. The membrane is washed extensively.
. The membrane is developed using and ECL detection system.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-HPGD antibody (Hydroxyprostaglandin Dehydrogenase 15-(NAD)) (ABIN268223) anti-Hydroxyprostaglandin Dehydrogenase 15-(NAD) (HPGD) antibody
Product cited in: Hughes, Otani, Yang et al.: "NAD+-dependent 15-hydroxyprostaglandin dehydrogenase regulates levels of bioactive lipids in non-small cell lung cancer." in: Cancer prevention research (Philadelphia, Pa.), Vol. 1, Issue 4, pp. 241-9, 2009 (PubMed).

Backlund, Mann, Holla et al.: "Repression of 15-hydroxyprostaglandin dehydrogenase involves histone deacetylase 2 and snail in colorectal cancer." in: Cancer research, Vol. 68, Issue 22, pp. 9331-7, 2008 (PubMed).

Background publications Ding, Tong, Liu et al.: "NAD+-linked 15-hydroxyprostaglandin dehydrogenase (15-PGDH) behaves as a tumor suppressor in lung cancer." in: Carcinogenesis, Vol. 26, Issue 1, pp. 65-72, 2004 (PubMed).

Giannoulias, Patel, Holloway et al.: "Differential changes in 15-hydroxyprostaglandin dehydrogenase and prostaglandin H synthase (types I and II) in human pregnant myometrium." in: The Journal of clinical endocrinology and metabolism, Vol. 87, Issue 3, pp. 1345-52, 2002 (PubMed).

Catalog No. ABIN268223
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