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NQO1 antibody (NAD(P)H Dehydrogenase, Quinone 1)

Details for Product anti-NQO1 Antibody No. ABIN268227, Supplier: Log in to see
Antigen
  • zgc:77191
  • wu:fb63c10
  • nqo1
  • DHQU
  • DIA4
  • DTD
  • NMOR1
  • NMORI
  • QR1
  • Dia4
  • AV001255
  • Dtd
  • Nmo-1
  • Nmo1
  • Nmor1
  • Ox-1
  • Ox1
  • Qr1
  • NADPH-d
  • NAD(P)H dehydrogenase, quinone 1
  • nqo1
  • NQO1
  • Bpet2092
  • Nqo1
Reactivity
Dog (Canine), Human, Mouse (Murine), Primate, Rat (Rattus)
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70
37
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14
8
5
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2
1
Host
Mouse
92
75
34
2
Clonality (Clone)
Monoclonal ()
Conjugate
This NQO1 antibody is un-conjugated
12
5
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2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
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1
1
1
1
1
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1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
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26
23
17
13
5
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2
1
1
1
1
Options
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Immunogen Full length recombinant NQO1 from human lung [UniProt# P15559]
Clone A180
Isotype IgG1
Specificity Does not cross-react with NQO2.
Purification Protein G purified
Alternative Name NQO-1 (NQO1 Antibody Abstract)
Background Gene Symbol: NQO1
Molecular Weight Theoretical MW: 31 kDa
Gene ID 1728
UniProt P15559
Application Notes Western Blot 1:1000, Simple Western 1:100, Flow Cytometry 1-2 μg/mL, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:1000, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:100This NQO1 Antibody (A180) is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections and Flow Cytometry. In Western blot a band can be seen at approx. 31 kDa representing NQO1. In ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm of human breast cancer tisSue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for NQO1 Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-NQO1 antibody (NAD(P)H Dehydrogenase, Quinone 1) (ABIN268227) Detection of NQO1 in A549 lysates using ABIN152344. Lane 1: 0.5 ug/ml. Lane 2: 2 ug...
Simple Western (SimWes) image for anti-NQO1 antibody (NAD(P)H Dehydrogenase, Quinone 1) (ABIN268227) Simple Western: NQO-1 Antibody (A180) [ABIN152344] - Simple Western lane view shows a...
 image for anti-NQO1 antibody (NAD(P)H Dehydrogenase, Quinone 1) (ABIN268227) Flow (Intracellular): NQO-1 Antibody (A180) [ABIN152344] - An intracellular stain was...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NQO1 antibody (NAD(P)H Dehydrogenase, Quinone 1) (ABIN268227) Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [ABIN152344] - Immunohistochemis...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NQO1 antibody (NAD(P)H Dehydrogenase, Quinone 1) (ABIN268227) Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [ABIN152344] - Analysis of NQO1 ...
Immunofluorescence (IF) image for anti-NQO1 antibody (NAD(P)H Dehydrogenase, Quinone 1) (ABIN268227) Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (A180) [ABIN152344] - NQO1 ant...
Product cited in: Qin, Du, Huang, Yin, Yang, Qin, Cao: "Sinomenine activation of Nrf2 signaling prevents hyperactive inflammation and kidney injury in a mouse model of obstructive nephropathy." in: Free radical biology & medicine, Vol. 92, pp. 90-9, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Qin, Yin, Yang, Zhang, Liu, Huang, Cao: "Sinomenine attenuates renal fibrosis through Nrf2-mediated inhibition of oxidative stress and TGFβ signaling." in: Toxicology and applied pharmacology, Vol. 304, pp. 1-8, 2016 (PubMed). Further details: Western Blotting

Qin, Du, Yin, Liu, Xu, Cao: "Nrf2 is essential for the anti-inflammatory effect of carbon monoxide in LPS-induced inflammation." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 64, Issue 7, pp. 537-48, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Boušová, Bártíková, Matoušková, Lněničková, Zappe, Valentová, Szotáková, Martin, Skálová: "Cranberry extract-enriched diets increase NAD(P)H:quinone oxidoreductase and catalase activities in obese but not in nonobese mice." in: Nutrition research (New York, N.Y.), Vol. 35, Issue 10, pp. 901-9, 2015 (PubMed).

Suyama, Watanabe, Sakabe, Otomo, Okada, Terayama, Imai, Mochida: "GRP78 suppresses lipid peroxidation and promotes cellular antioxidant levels in glial cells following hydrogen peroxide exposure." in: PLoS ONE, Vol. 9, Issue 1, pp. e86951, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Zhang, Liang, Shi, Wang, Chen, Kang, Zhu, Mi: "Estrogen receptor and PI3K/Akt signaling pathway involvement in S-(-)equol-induced activation of Nrf2/ARE in endothelial cells." in: PLoS ONE, Vol. 8, Issue 11, pp. e79075, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Wang, Zhu, Kim, Li, Huang, Saleem, Li, Xu, Dore, Cao: "Histone deacetylase inhibition activates transcription factor Nrf2 and protects against cerebral ischemic damage." in: Free radical biology & medicine, Vol. 52, Issue 5, pp. 928-36, 2012 (PubMed).

Gao, Singh, Macri, Reynolds, Singhal, Biswal, Spannhake: "Antioxidant components of naturally-occurring oils exhibit marked anti-inflammatory activity in epithelial cells of the human upper respiratory system." in: Respiratory research, Vol. 12, pp. 92, 2011 (PubMed).

Solis, Behrens, Dong, Suraokar, Ozburn, Moran, Corvalan, Biswal, Swisher, Bekele, Minna, Stewart, Wistuba: "Nrf2 and Keap1 abnormalities in non-small cell lung carcinoma and association with clinicopathologic features." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 14, pp. 3743-53, 2010 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Cho, Imani, Miller-DeGraff, Walters, Melendi, Yamamoto, Polack, Kleeberger: "Antiviral activity of Nrf2 in a murine model of respiratory syncytial virus disease." in: American journal of respiratory and critical care medicine, Vol. 179, Issue 2, pp. 138-50, 2009 (PubMed).

Salama, Kamel, Diaz-Arrastia, Xu, Veenstra, Salih, Botting, Kumar: "Effect of tumor necrosis factor-alpha on estrogen metabolism and endometrial cells: potential physiological and pathological relevance." in: The Journal of clinical endocrinology and metabolism, Vol. 94, Issue 1, pp. 285-93, 2009 (PubMed).

Li, Kim, Godoy, Thiantanawat, Trudel, Wogan: "Nitric oxide activation of Keap1/Nrf2 signaling in human colon carcinoma cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 34, pp. 14547-51, 2009 (PubMed).

Malhotra, Thimmulappa, Vij, Navas-Acien, Sussan, Merali, Zhang, Kelsen, Myers, Wise, Tuder, Biswal: "Heightened endoplasmic reticulum stress in the lungs of patients with chronic obstructive pulmonary disease: the role of Nrf2-regulated proteasomal activity." in: American journal of respiratory and critical care medicine, Vol. 180, Issue 12, pp. 1196-207, 2009 (PubMed).

Lee, Bhora, Sun, Cheng, Arguiri, Solomides, Chatterjee, Christofidou-Solomidou: "Dietary flaxseed enhances antioxidant defenses and is protective in a mouse model of lung ischemia-reperfusion injury." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 294, Issue 2, pp. L255-65, 2008 (PubMed).

Slitt, Cherrington, Dieter, Aleksunes, Scheffer, Huang, Moore, Klaassen: "trans-Stilbene oxide induces expression of genes involved in metabolism and transport in mouse liver via CAR and Nrf2 transcription factors." in: Molecular pharmacology, Vol. 69, Issue 5, pp. 1554-63, 2006 (PubMed).

Dehn, Siegel, Zafar, Reigan, Swann, Moody, Ross et al.: "5-Methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione, a mechanism-based inhibitor of NAD(P)H:quinone oxidoreductase 1, exhibits activity against human pancreatic cancer in vitro and in ..." in: Molecular cancer therapeutics, Vol. 5, Issue 7, pp. 1702-9, 2006 (PubMed). (Sample species: Human). Further details: Western Blotting

Zhang, Schulz, Li, Wang, Zotz, Wen, Siegel, Ross, Gabbert, Sarbia: "Association of NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism with esophageal squamous cell carcinoma in a German Caucasian and a northern Chinese population." in: Carcinogenesis, Vol. 24, Issue 5, pp. 905-9, 2003 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Anwar, Dehn, Siegel, Kepa, Tang, Pietenpol, Ross: "Interaction of human NAD(P)H:quinone oxidoreductase 1 (NQO1) with the tumor suppressor protein p53 in cells and cell-free systems." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10368-73, 2003 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Siegel, Anwar, Winski, Kepa, Zolman, Ross: "Rapid polyubiquitination and proteasomal degradation of a mutant form of NAD(P)H:quinone oxidoreductase 1." in: Molecular pharmacology, Vol. 59, Issue 2, pp. 263-8, 2001 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Siegel, Ross: "Immunodetection of NAD(P)H:quinone oxidoreductase 1 (NQO1) in human tissues." in: Free radical biology & medicine, Vol. 29, Issue 3-4, pp. 246-53, 2001 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)