Dicer 1, Ribonuclease Type III (DICER1) (AA 132-150) antibody

Details for Product No. ABIN268305, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

AA 132-150
(37), (13), (8), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(95), (74), (42), (2), (1), (1), (1), (1), (1)
(58), (56), (5), (1)
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(85), (40), (24), (20), (19), (17), (11), (7), (4), (1), (1), (1)
Pubmed 3 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Immunogen Rabbit anti-mouse Dicer polyclonal antibody was raised against a peptide corresponding to amino acids 132-150 and 1017-1033 of mouse Dicer (EDL18787).
Isotype IgG
Specificity In mouse ovary, a 217 kDa band is observed.
Cross-Reactivity (Details) Expected to cross-react with Human,rat,due to over 90% sequence identity with immunogen, but testing shows no response in human,rat,ovary at the recommended testing conditions. Not yet tested in other species.
Purification Protein G purified
Alternative Name DICER1 (DICER1 Antibody Abstract)
Background Dicer is an RNaseIII-like enzyme which cleaves double-stranded RNA into 21-23 ntRNAs. These RNAs are referred to as siRNA (short interfering RNA). It is believed to beessential in siRNA-mediated gene suppression.
Gene Symbol: DICER1
Gene ID 23405
Pathways Regulatory RNA Pathways
Application Notes WB: Use at a dilution of 1/500 - 1/1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, Western Blot 0.5-3 µg/mL
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Product cited in: Doi, Zenno, Ueda et al.: "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors." in: Current biology : CB, Vol. 13, Issue 1, pp. 41-6, 2003 (PubMed).

Provost, Silverstein, Dishart et al.: "Dicer is required for chromosome segregation and gene silencing in fission yeast cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16648-53, 2002 (PubMed).

Zhang, Kolb, Brondani et al.: "Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP." in: The EMBO journal, Vol. 21, Issue 21, pp. 5875-85, 2002 (PubMed).