tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) antibody

Antigen: tRNA Aspartic Acid Methyltransferase 1 (TRDMT1)

Synonyms: ecat7, D6Ertd14e, MGC102308, DNMT3L, MGC53207, MGC89267

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN268327

Additional Information

Alternative name: DNMT2 / TRDMT1

Gene ID: 1787, 13434

Immunogen: This antibody was raised against synthetic peptides corresponding amino acids 39-53and 361-376 of mouse Dnmt2.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG

Description: Methylation of DNA at cytosine residues plays an important role in regulation of geneexpression, genomic imprinting and is essential for mammalian development.Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulkgenomic DNA may be linked with development of cancer. To date, 3 families ofmammalian DNA methyltransferase genes have been identified which include Dnmt1,Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells andinactivation of this gene causes global demethylation of genomic DNA and embryoniclethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does notaffect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequencemotifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the largeN-terminal regulatory domain common to other eukaryotic methyltransferases. Alternate Names: anti-DNA methyltransferase 2 antibody.

Specificity: Species Reactivity: Cross-reacts with Human and Mouse. Not yet tested in other species. Localization: Nuclear.

Application Details

Application Notes: Suggested working dilutions: Western Blot 2 ug/ml. Positive Controls: mouse testis

Concentration: 0.5 mg/ml

Buffer: PBS. Preservative: Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Publications

Okano, Xie, Li: "Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells." in: Nucleic acids research, Vol. 26, Issue 11, pp. 2536-40, 1998 (PubMed).

Kawasaki, Taira: "Induction of DNA methylation and gene silencing by short interfering RNAs in human cells." in: Nature, Vol. 431, Issue 7005, pp. 211-7, 2004 (PubMed).