Tachykinin Receptor 1 (TACR1) (C-Term) antibody

Details for Product No. ABIN268373
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Antigen
Synonyms NK1R, NKIR, SPR, TAC1R, Nk1r, Spr, Tac1r, nk1R, Tacr1, ASPR
Epitope
C-Term
(9), (9), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Guinea Pig, Mouse (Murine), Rat (Rattus)
(70), (37), (33), (16), (16), (13), (12), (1), (1), (1), (1)
Host
Rabbit
(75), (5), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(50), (28), (24), (14), (10), (9), (9), (6), (3), (1)
Pubmed 16 references available
Quantity 0.025 mL
Shipping to United States (Change)
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Catalog No. ABIN268373
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Immunogen A synthetic peptide corresponding to the C-terminus of rat Neurokinin 1 Receptor, conjugated to bovine thyroglobulin. Immunogen sequence has 92% homology to canine.
Specificity NB300-101 is specific to the NK-1 receptor. Staining of cells transfected with NK-1 was abolished by preabsorption of the antisera with NK-1. Specificity was also verified by the localization of immunohistochemical staining with NB300-101 in neurons in
Purification Whole antisera
Background The tachykinins belong to an evolutionary conserved family of peptide neurotransmittersthat share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an establishedrole in neurotransmission. The mammalian tachykinins include substance P, neurokininA (NKA) and neurokinin B (NKB) which exert their effects by binding to specificreceptors. Tachykinin peptides are important in the mediation of many physiologicaland pathological processes including inflammation, pain, migraine headache and allergyinduced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 whichhave preferential affinities for SP, NKA and NKB respectively. All three receptors sharea high degree of sequence homology, have seven transmembrane spanning domainsand similar signal transduction mechanisms (e.g. G-protein coupled activation ofphospholipase C). Alternate Names: anti-NK1 receptor antibody, anti-NK1R antibody, anti-SPR antibody, anti-Substance Preceptor antibody, anti-TAC1R antibody, anti-Tachykinin receptor 1 antibody,anti-TACR1 antibody, anti-NK-1r antibody.
Gene Symbol: TACR1
Gene ID 6869
UniProt P14600
Research Area Hormones, Receptors
Application Notes This Neurokinin 1 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. For ICC/IF staining, please see customer the review. Use in Immunohistochemistry on paraffin embedded sections was reported in scientific literature (PMID: 23792204).
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry 1:1000, Immunohistochemistry-Frozen 1:1000, Immunohistochemistry-Paraffin, Western Blot 1:100-1:2000
Protocol Protocol specific for Neurokinin 1 Receptor Antibody Immunohistochemistry1) Perfuse rat through the ascending aorta with 500 mL. of 0.1 M phosphate buffered saline (PBS) (pH 7.4 at 4 degrees C), followed by 750 mL. of PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9, 4 degrees C). Tissue of interest is then dissected and post-fixed in PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9 at 4 degrees C) for at least 4 hours, followed by fixation in PBS containing 30 % sucrose (pH 7.4, 4 degrees C) for at least 24 hours. Until the tissue sinks.
2) Cut fixed sections at 60 um using a sliding microtome. 3) If using culture cells, remove media and perform 2-3 washed in PBS (pH 7.
) -use same protocol without agitation. 4) Place tissue section in microcentrifuge tubes containing 1 mL. Of PBS, pH 7.4 as they are being cut. Wash sections (in PBS) on an upright rotator for 10-15 minutes.
) Remove PBS and add 1 mL. of blocking solution (PBS + 1 % normal donkey serum (NDS) + 0.3 % triton X-100).
) Incubate tissue sections in blocking solution for 30 minutes at room temperature (RT) on rotator. 7) Remove blocking solution and add anti-neurokinin-1 receptor or anti-Neurokinin-3 receptor antibody +PBS + 1 % NDS + 0.3 % triton X-100 + NB 300-101/102 at 1:100~1:10008) Incubate overnight on rotator at RT.
) Remove primary antisera and perform 3 x 10 minute PBS washes (1ml volume) on rotator.
) Add 1 mL. of secondary antibody and incubate for 2 hours at RT on rotator. -PBS + 1 % NDS + 0.3 % triton X 100 + anti-rabbit Cy3 (Jackson Immunoresearch Labs) at 1:60011) Perform 3 X 10 minute PBS washes (1 mL volume) on rotator12) Mount tissue sections on gelatin coated slides and allow tissue to dry13) Run slides through alcohol gradients (70 %, 90 %, 100 %, xylene) leaving slide in each alcohol and xylene for 2 minutes14) Coverslip with DPX mountant (FLUKA) ** Note: 24 well plates may be substituted for microcentrifuge tubes. Use flat top bench rotator instead of upright rotator.
Restrictions For Research Use only
Format Liquid
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General Vigna, Bowden, McDonald et al.: "Characterization of antibodies to the rat substance P (NK-1) receptor and to a chimeric substance P receptor expressed in mammalian cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 14, Issue 2, pp. 834-45, 1994 (PubMed).

Mantyh, Allen, Ghilardi et al.: "Rapid endocytosis of a G protein-coupled receptor: substance P evoked internalization of its receptor in the rat striatum in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 7, pp. 2622-6, 1995 (PubMed).

Mantyh, DeMaster, Malhotra et al.: "Receptor endocytosis and dendrite reshaping in spinal neurons after somatosensory stimulation." in: Science (New York, N.Y.), Vol. 268, Issue 5217, pp. 1629-32, 1995 (PubMed).

Mantyh, Rogers, Ghilardi et al.: "Differential expression of two isoforms of the neurokinin-1 (substance P) receptor in vivo." in: Brain research, Vol. 719, Issue 1-2, pp. 8-13, 1997 (PubMed).

Mantyh, Pappas, Lapp et al.: "Substance P activation of enteric neurons in response to intraluminal Clostridium difficile toxin A in the rat ileum." in: Gastroenterology, Vol. 111, Issue 5, pp. 1272-80, 1996 (PubMed).

Allen, Rogers, Ghilardi et al.: "Noxious cutaneous thermal stimuli induce a graded release of endogenous substance P in the spinal cord: imaging peptide action in vivo." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 15, pp. 5921-7, 1997 (PubMed).

Mantyh, Rogers, Honore et al.: "Inhibition of hyperalgesia by ablation of lamina I spinal neurons expressing the substance P receptor." in: Science (New York, N.Y.), Vol. 278, Issue 5336, pp. 275-9, 1997 (PubMed).

Anderson, Seybold: "Phosphorylated cAMP response element binding protein increases in neurokinin-1 receptor-immunoreactive neurons in rat spinal cord in response to formalin-induced nociception." in: Neuroscience letters, Vol. 283, Issue 1, pp. 29-32, 2000 (PubMed).

Tooney, Au, Chahl: "Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex." in: Neuroscience letters, Vol. 283, Issue 3, pp. 185-8, 2000 (PubMed).

Setou, Nakagawa, Seog et al.: "Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport." in: Science (New York, N.Y.), Vol. 288, Issue 5472, pp. 1796-802, 2000 (PubMed).

Yip, Chahl: "Localization of NK1 and NK3 receptors in guinea-pig brain." in: Regulatory peptides, Vol. 98, Issue 1-2, pp. 55-62, 2001 (PubMed).

Ma, Huang, Chien et al.: "Temporal decrease in renal sensory responses in rats after chronic ligation of the bile duct." in: American journal of physiology. Renal physiology, Vol. 283, Issue 1, pp. F164-72, 2002 (PubMed).

Mounir, Parent: "The expression of neurokinin-1 receptor at striatal and pallidal levels in normal human brain." in: Neuroscience research, Vol. 44, Issue 1, pp. 71-81, 2002 (PubMed).

Kc, Haxhiu, Tolentino-Silva et al.: "Paraventricular vasopressin-containing neurons project to brain stem and spinal cord respiratory-related sites." in: Respiratory physiology & neurobiology, Vol. 133, Issue 1-2, pp. 75-88, 2002 (PubMed).

Commons, Connolley, Valentino: "A neurochemically distinct dorsal raphe-limbic circuit with a potential role in affective disorders." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 28, Issue 2, pp. 206-15, 2003 (PubMed).

Broccardo, Linari, Agostini et al.: "Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 291, Issue 3, pp. G518-24, 2006 (PubMed).

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