You are viewing an incomplete version of our website. Please click to reload the website as full version.

Tachykinin Receptor 3 (TACR3) (C-Term) antibody

Details for Product No. ABIN268379, Supplier: Log in to see
Antigen
  • HH11
  • NK-3R
  • NK3R
  • NKR
  • TAC3RL
  • Nk3r
  • Nkr
  • Tac3r
  • Nmkr
  • tachykinin receptor 3
  • TACR3
  • Tacr3
Alternatives
anti-Mouse (Murine) Tachykinin Receptor 3 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term
27
11
9
8
4
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Guinea Pig, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
83
42
37
3
1
1
1
1
1
1
1
Host
Rabbit
91
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro))
53
30
20
19
14
12
4
2
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen A synthetic peptide at the C-terminus of rat Neurokinin B Receptor, conjugated to bovine thyroglobulin. [UniProt# P16177]
Specificity This antibody is specific to the NK-3 receptor. Specificity was verified by the localization of immunohistochemical staining as well as pre-absorption studies with NK-3 peptide. (1)
No Cross-Reactivity Human
Purification Unpurified
Background Gene Symbol: TACR3
Gene ID 6870
UniProt P16177
Pathways Feeding Behaviour
Application Notes Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Immunohistochemistry Free-FloatingThis Neurokinin B Receptor antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections.Use in Immunohistochemistry- free floating reported in scientific literature (PMID 25496429). It is not useful for Western blot.

Not for use in: Western Blot
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry Protocol specific for Neurokinin B Receptor Antibody Procedure Guide for NB 300-102 Polyclonal Anti-Neurokinin-3 (NK-B) Receptor Immunohistochemistry1) Tissue of interest is dissected and post-fixed in PBS containing 4 % formaldehyde or PBS containing 4 % formaldehyde + 0.1 % gluteraldehyde**, followed by fixation in PBS containing 20 % sucrose, overnight at 4C. Until the tissue sinks.
2) Cut fixed sections at 15 mm or 40 mm using a cryostat or sliding microtome, depending on incubation technique [for cryosections use 15 mm and for free-floating sections use 40 mm].
) If using culture cells, remove media and perform 2-3 washed in PBS (pH 7.
) -use same protocol without agitation. 4) Wash sections in PBS for 10 minutes, 3 times.
) Block the tissue with 1 % BSA / 1 % goat serum / 0.3 % triton X-100 in 0.1M PBS, for 1 hour at RT. 6) Dilute anti-NK-3 receptor in blocking buffer at 1:1000 for cryosections and 1:5000 for free-floating sections.
) Incubate sections overnight at 4C.
) Wash sections in PBS for 10 minutes, 3 times.
) Dilute secondary antibody (1:400 ?? Alexa 568 [Rho] goat anti-rabbit, from Molecular Probes, Inc.) in blocking buffer and incubate sections for 2 hours at RT.
) Wash sections in PB (not PBS) for 10 minutes, 3 times.
) Mount coverslips to slides using Fluoromount-G (Southern Biotech Assoc, cat# 0100-01)**Note: Mouse dorsal horn and cerebral cortex were used as positive control section. *Tissues fixed in 4 % paraformaldehyde + 0.1 % gluteraldehyde are used for EM analysis
Restrictions For Research Use only
Format Liquid
Buffer Whole antisera
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Product cited in: Hsu, Morton, Guy, Wang, Turner: "Dorsal Medial Habenula Regulation of Mood-Related Behaviors and Primary Reinforcement by Tachykinin-Expressing Habenula Neurons." in: eNeuro, Vol. 3, Issue 3, 2016 (PubMed).

Ahn, Fergani, Coolen, Padmanabhan, Lehman: "Prenatal testosterone excess decreases neurokinin 3 receptor immunoreactivity within the arcuate nucleus KNDy cell population." in: Journal of neuroendocrinology, Vol. 27, Issue 2, pp. 100-10, 2015 (PubMed). (Sample species: Sheep (Ovine)). Further details: Immunohistochemistry (Free Floating)

Sawai, Iijima, Ozawa, Matsuzaki: "Neurokinin B- and kisspeptin-positive fibers as well as tuberoinfundibular dopaminergic neurons directly innervate periventricular hypophyseal dopaminergic neurons in rats and mice." in: Neuroscience research, Vol. 84, pp. 10-8, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Nuhn, Fuerst: "Developmental localization of adhesion and scaffolding proteins at the cone synapse." in: Gene expression patterns : GEP, Vol. 16, Issue 1, pp. 36-50, 2014 (PubMed).

de Andrade, Long, Fleming, Li, Fuerst: "DSCAM localization and function at the mouse cone synapse." in: The Journal of comparative neurology, Vol. 522, Issue 11, pp. 2609-33, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Nivison-Smith, Sun, Fletcher, Marc, Kalloniatis: "Mapping kainate activation of inner neurons in the rat retina." in: The Journal of comparative neurology, Vol. 521, Issue 11, pp. 2416-38, 2013 (PubMed). (Sample species: Rat (Rattus)).

Hether, Misono, Lessard: "The neurokinin-3 receptor (NK3R) antagonist SB222200 prevents the apomorphine-evoked surface but not nuclear NK3R redistribution in dopaminergic neurons of the rat ventral tegmental area." in: Neuroscience, Vol. 247, pp. 12-24, 2013 (PubMed). (Sample species: Rat (Rattus)).

Sawai, Iijima, Takumi, Matsumoto, Ozawa: "Immunofluorescent histochemical and ultrastructural studies on the innervation of kisspeptin/neurokinin B neurons to tuberoinfundibular dopaminergic neurons in the arcuate nucleus of rats." in: Neuroscience research, Vol. 74, Issue 1, pp. 10-6, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Misono, Lessard: "Apomorphine-evoked redistribution of neurokinin-3 receptors in dopaminergic dendrites and neuronal nuclei of the rat ventral tegmental area." in: Neuroscience, Vol. 203, pp. 27-38, 2012 (PubMed).

Amstalden, Coolen, Hemmerle, Billings, Connors, Goodman, Lehman et al.: "Neurokinin 3 receptor immunoreactivity in the septal region, preoptic area and hypothalamus of the female sheep: colocalisation in neurokinin B cells of the arcuate nucleus but not in ..." in: Journal of neuroendocrinology, Vol. 22, Issue 1, pp. 1-12, 2010 (PubMed). (Sample species: Sheep (Ovine)). Further details: Immunocytochemistry,Immunofluorescence

Krajewski, Burke, Anderson, McMullen, Rance: "Forebrain projections of arcuate neurokinin B neurons demonstrated by anterograde tract-tracing and monosodium glutamate lesions in the rat." in: Neuroscience, Vol. 166, Issue 2, pp. 680-97, 2010 (PubMed).

Le Brun, Dufour, Crest, Szabó, Erdelyi, Baude: "Differential expression of Nk1 and NK3 neurokinin receptors in neurons of the nucleus tractus solitarius and the dorsal vagal motor nucleus of the rat and mouse." in: Neuroscience, Vol. 152, Issue 1, pp. 56-64, 2008 (PubMed).

Oh, Khan, Novelli, Khanna, Strettoi, Swaroop: "Transformation of cone precursors to functional rod photoreceptors by bZIP transcription factor NRL." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 5, pp. 1679-84, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Lévesque, Wallman, Parent, Sík, Parent: "Neurokinin-1 and neurokinin-3 receptors in primate substantia nigra." in: Neuroscience research, Vol. 57, Issue 3, pp. 362-71, 2007 (PubMed).

Beltran, Hammond, Acland, Aguirre: "A frameshift mutation in RPGR exon ORF15 causes photoreceptor degeneration and inner retina remodeling in a model of X-linked retinitis pigmentosa." in: Investigative ophthalmology & visual science, Vol. 47, Issue 4, pp. 1669-81, 2006 (PubMed).

Broccardo, Linari, Agostini, Amadoro, Carpino, Ciotti, Petrella, Petrozza, Severini, Improta: "Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 291, Issue 3, pp. G518-24, 2006 (PubMed). (Sample species: Rat (Rattus)).

Haley, Flynn: "Agonist and hypertonic saline-induced trafficking of the NK3-receptors on vasopressin neurons within the paraventricular nucleus of the hypothalamus." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 290, Issue 5, pp. R1242-50, 2006 (PubMed).

Lévesque, Parent, Parent: "Cellular and subcellular localization of neurokinin-1 and neurokinin-3 receptors in primate globus pallidus." in: The European journal of neuroscience, Vol. 23, Issue 10, pp. 2760-72, 2006 (PubMed).

Burke, Letts, Krajewski, Rance: "Coexpression of dynorphin and neurokinin B immunoreactivity in the rat hypothalamus: Morphologic evidence of interrelated function within the arcuate nucleus." in: The Journal of comparative neurology, Vol. 498, Issue 5, pp. 712-26, 2006 (PubMed).

Mukda, Chetsawang, Govitrapong, Schmidt, Hay-Schmidt, Møller: "Tachykinins and tachykinin-receptors in the rat pineal gland." in: The European journal of neuroscience, Vol. 21, Issue 10, pp. 2743-51, 2005 (PubMed). (Sample species: Rat (Rattus)).