Tachykinin Receptor 3 (TACR3) (C-Term) antibody

Details for Product No. ABIN268379
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Antigen
Synonyms HH11, NK-3R, NK3R, NKR, TAC3RL, Nk3r, Nkr, Tac3r, Nmkr
Epitope
C-Term
(19), (11), (7), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Guinea Pig
(76), (47), (41), (25), (24), (24), (24), (24), (4), (1)
Host
Rabbit
(80)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
(48), (27), (20), (20), (10), (10), (4), (3), (2), (1)
Pubmed 9 references available
Quantity 0.025 mL
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Catalog No. ABIN268379
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Immunogen A synthetic peptide at the C-terminus of rat Neurokinin B Receptor, conjugated to bovine thyroglobulin.
Specificity NB300-102 is specific to the NK-3 receptor. Specificity was verified by the localization of immunohistochemical staining as well as pre-absorption studies with NK-3 peptide. (1)
No Cross-Reactivity Human
Cross-Reactivity (Details) Customer feedback suggests that human reactivity is negative. Sheep reactivity reported in scientific literature (PMID: 19912479).
Purification Whole antisera
Background The tachykinins belong to an evolutionary conserved family of peptide neurotransmittersthat share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an establishedrole in neurotransmission. The mammalian tachykinins include substance P, neurokininA (NKA) and neurokinin B (NKB) which exert their effects by binding to specificreceptors. Tachykinin peptides are important in the mediation of many physiologicaland pathological processes including inflammation, pain, migraine headache and allergyinduced asthma.Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 whichhave preferential affinities for SP, NKA and NKB respectively. All three receptors sharea high degree of sequence homology, have seven transmembrane spanning domainsand similar signal transduction mechanisms (e.g. G-protein coupled activation ofphospholipase C). Alternate Names: anti-Neurokinin B receptor antibody, anti-Neuromedin K Receptor antibody, anti-NK3receptor antibody, anti-NK3R antibody, anti-TAC3R antibody, anti-TAC3RL antibody,anti-Tachykinin receptor 3 antibody, anti-TACR3 antibody.
Gene Symbol: TACR3
Gene ID 6870
UniProt P16177
Research Area Receptors, Neurotransmitters
Application Notes This Neurokinin B Receptor antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. It is not useful for Western blot.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Western Blot (Negative)
Protocol Immunohistochemistry Protocol specific for Neurokinin B Receptor Antibody Procedure Guide for NB 300-102 Polyclonal Anti-Neurokinin-3 (NK-B) Receptor Immunohistochemistry1) Tissue of interest is dissected and post-fixed in PBS containing 4 % formaldehyde or PBS containing 4 % formaldehyde + 0.1 % gluteraldehyde**, followed by fixation in PBS containing 20 % sucrose, overnight at 4C. Until the tissue sinks.
2) Cut fixed sections at 15 mm or 40 mm using a cryostat or sliding microtome, depending on incubation technique [for cryosections use 15 mm and for free-floating sections use 40 mm].
) If using culture cells, remove media and perform 2-3 washed in PBS (pH 7.
) -use same protocol without agitation. 4) Wash sections in PBS for 10 minutes, 3 times.
) Block the tissue with 1 % BSA / 1 % goat serum / 0.3 % triton X-100 in 0.1M PBS, for 1 hour at RT. 6) Dilute anti-NK-3 receptor in blocking buffer at 1:1000 for cryosections and 1:5000 for free-floating sections.
) Incubate sections overnight at 4C.
) Wash sections in PBS for 10 minutes, 3 times.
) Dilute secondary antibody (1:400 ?? Alexa 568 [Rho] goat anti-rabbit, from Molecular Probes, Inc.) in blocking buffer and incubate sections for 2 hours at RT.
) Wash sections in PB (not PBS) for 10 minutes, 3 times.
) Mount coverslips to slides using Fluoromount-G (Southern Biotech Assoc, cat# 0100-01)**Note: Mouse dorsal horn and cerebral cortex were used as positive control section. *Tissues fixed in 4 % paraformaldehyde + 0.1 % gluteraldehyde are used for EM analysis
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General Tooney, Au, Chahl: "Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex." in: Neuroscience letters, Vol. 283, Issue 3, pp. 185-8, 2000 (PubMed).

Yip, Chahl: "Localization of NK1 and NK3 receptors in guinea-pig brain." in: Regulatory peptides, Vol. 98, Issue 1-2, pp. 55-62, 2001 (PubMed).

Dick, tom Dieck, Altrock et al.: "The presynaptic active zone protein bassoon is essential for photoreceptor ribbon synapse formation in the retina." in: Neuron, Vol. 37, Issue 5, pp. 775-86, 2003 (PubMed).

Feigenspan, Janssen-Bienhold, Hormuzdi et al.: "Expression of connexin36 in cone pedicles and OFF-cone bipolar cells of the mouse retina." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 13, pp. 3325-34, 2004 (PubMed).

Howe, Somponpun, Sladek: "Role of neurokinin 3 receptors in supraoptic vasopressin and oxytocin neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 45, pp. 10103-10, 2004 (PubMed).

Haley, Flynn: "Agonist and hypertonic saline-induced trafficking of the NK3-receptors on vasopressin neurons within the paraventricular nucleus of the hypothalamus." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 290, Issue 5, pp. R1242-50, 2006 (PubMed).

Beltran, Hammond, Acland et al.: "A frameshift mutation in RPGR exon ORF15 causes photoreceptor degeneration and inner retina remodeling in a model of X-linked retinitis pigmentosa." in: Investigative ophthalmology & visual science, Vol. 47, Issue 4, pp. 1669-81, 2006 (PubMed).

Broccardo, Linari, Agostini et al.: "Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 291, Issue 3, pp. G518-24, 2006 (PubMed).

Oh, Khan, Novelli et al.: "Transformation of cone precursors to functional rod photoreceptors by bZIP transcription factor NRL." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 5, pp. 1679-84, 2007 (PubMed).

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