Laminin (LN) (pan) antibody

Details for Product No. ABIN268409, Supplier: Log in to see
Antigen
  • LAMC1
  • laminin
  • laminin, beta 2 (laminin S)
  • LAMB2
Epitope
pan
10
4
3
3
1
1
1
1
Reactivity
Human, Invertebrate, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
99
48
38
17
13
11
10
10
4
4
3
3
1
1
1
1
1
Host
Rabbit
83
29
10
3
Clonality
Polyclonal
Conjugate
Un-conjugated
9
2
2
2
2
2
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
75
69
54
47
34
28
26
18
10
8
4
2
1
1
1
Options
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Immunogen Laminin isolated from mouse EHS tumor. [UniProt# P19137]
Specificity This is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
Purification IgG purified
Alternative Name Laminin
Background Gene Symbol: LAMA1
Gene ID 284217
UniProt P19137
Research Area Neurology, Angiogenesis, Extracellular Matrix
Application Notes Western Blot 1:100-1:5000, Immunohistochemistry 1:500-1:2000, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:500-1:2000, Immunohistochemistry-Frozen 1:500-1:2000, FunctionalThis Laminin antibody can be used for Immunocytochemistry/Immunofluorescence, Functional assays, Immunohistochemistry paraffin and frozen sections, and Western blotting where it detects bands at 200 and 400 kDa. The antibody functionally inhibits Laminin in mouse and rat. It binds to Laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-Paraffin protocol for Laminin Antibody Laminin Immunohistohemistry ABC/HRPThe fixation is routine paraformaldehyde or formalin fixation of tissue prior to paraffin embedding. However, the staining procedure must include an antigen retrieval step pretreating the deparaffinized sections with pepsin. This is required for all laminin antibodies used on paraffin tissue.Proteolytic antigen retrieval:Apply 250 µL of pepsin at 4mg/mL in 0.01M HCL (pH ~2.0).Incubate for 60 minutes at 37 degrees C in a humid chamber.Wash x2 in distilled H20, 5 min. each wash.Mount paraffin sections on Fisher Plus slides. Bake for >2 hours at 50C.
1. Deparaffinize mounted sections in xylene: 2 changes 5 min each, and a 3rd change for 10 min.
. Exchange solvent to ethanol with 2 changes of 100 % EtOH for 5 min each.
. Quench endogenous peroxidase for 30 min in 100 % methanol + 1 % H2O2.
. Hydrate to H2O in graded ethanol series. 5 min. in 95 % EtOH, 5 min. in 70 % EtOH, 5 min. in running H2O. Rinse with dH2O. Circumscribe the sections with PAP Pen.
. Unmask antigen by proteolysis. Cover sections with 100ul of 4mg/ml of pepsin (Sigma #P6887) dissolved in 0.01M HCl. Treat for 1 hr at 37C in humidified chamber. Rinse in running H2O.
. Blocking: Block background staining by covering sections with 100ul of PBS containing 10 % normal swine serum (Blocking
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Laminin (LN) (pan) antibody (ABIN268409) Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue...
Western Blotting (WB) image for anti-Laminin (LN) (pan) antibody (ABIN268409) Detection of laminin by Western blot using ABIN152490.
Western Blotting (WB) image for anti-Laminin (LN) (pan) antibody (ABIN268409) Western Blot: Laminin Antibody [ABIN152490] - Analysis of rat heart cells lysates (la...
Immunohistochemistry (IHC) image for anti-Laminin (LN) (pan) antibody (ABIN268409) Immunohistochemistry: Laminin Antibody [ABIN152490] - Staining of mouse section of co...
Immunofluorescence (IF) image for anti-Laminin (LN) (pan) antibody (ABIN268409) Immunocytochemistry/Immunofluorescence: Laminin Antibody [ABIN152490] - IF Confocal a...
Immunofluorescence (IF) image for anti-Laminin (LN) (pan) antibody (ABIN268409) Immunocytochemistry/Immunofluorescence: Laminin Antibody [ABIN152490] - Detection of ...
Product cited in: Gelmedin, Morel, Hahnel, Cailliau, Dissous, Grevelding: "Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival." in: PLoS pathogens, Vol. 13, Issue 1, pp. e1006147, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC)

Shojaie, Lee, Wang, Ackerley, Post: "Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds." in: Journal of visualized experiments : JoVE, Issue 111, 2016 (PubMed).

Mori, Yao, Learman, Kurozumi, Ishida, Ramakrishnan, Overmyer, Xue, Cawthorn, Reid, Taylor, Ning, Shah, MacDougald: "Induction of WNT11 by hypoxia and hypoxia-inducible factor-1α regulates cell proliferation, migration and invasion." in: Scientific reports, Vol. 6, pp. 21520, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Fu, Song, Wen, Lu, Cui: "Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice." in: Nutrients, Vol. 8, Issue 5, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Castillo-Melendez, Yawno, Allison, Jenkin, Wallace, Miller: "Cerebrovascular adaptations to chronic hypoxia in the growth restricted lamb." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 45, pp. 55-65, 2015 (PubMed). (Sample species: Sheep (Ovine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Garvey, Russ, Skelding, Dugle, Edens: "Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats." in: Physiological reports, Vol. 3, Issue 2, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Pandya, Serra, Green, Gridley: "pKC modulates integrin expression that contributes to fibrotic changes in irradiated thyroid tissue." in: In vivo (Athens, Greece), Vol. 29, Issue 2, pp. 177-88, 2015 (PubMed).

Van der Donckt, Roth, Vanhoutte, Blockx, Bink, Ritz, Pintelon, Timmermans, Bauters, Martinet, Daemen, Verhoye, De Meyer: "Fibrillin-1 impairment enhances blood-brain barrier permeability and xanthoma formation in brains of apolipoprotein E-deficient mice." in: Neuroscience, Vol. 295, pp. 11-22, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Wharton, Stefanov, King, Kearney: "Antibodies generated against Streptococci protect in a mouse model of disseminated aspergillosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 9, pp. 4387-96, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Patel, Kearney: "Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 12, pp. 5838-50, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Yang, Chiao, Lee, Chen, Yang, Shen, Ma: "An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 3, pp. 618-28, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry

Zhao, Sun, Zhang, Ting, Song, Gonzales, Aronowski: "Polymorphonuclear neutrophil in brain parenchyma after experimental intracerebral hemorrhage." in: Translational stroke research, Vol. 5, Issue 5, pp. 554-61, 2014 (PubMed). (Sample species: Rat (Rattus)).

Mancuso, Gualerzi, Boschetti, Loy, Cao: "Decellularized ovine arteries as small-diameter vascular grafts." in: Biomedical materials (Bristol, England), Vol. 9, Issue 4, pp. 45011, 2014 (PubMed). (Sample species: Sheep (Ovine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Alhusban, Fouda, Bindu Pillai, Ishrat, Soliman, Fagan: "Compound 21 is pro-angiogenic in the brain and results in sustained recovery after ischemic stroke." in: Journal of hypertension, Vol. 33, Issue 1, pp. 170-80, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Lifshitz, Benromano, Weiss, Blanga-Kanfi, Frenkel: "Insulin-degrading enzyme deficiency accelerates cerebrovascular amyloidosis in an animal model." in: Brain, behavior, and immunity, Vol. 30, pp. 143-9, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Millward, Schnorr, Taupitz, Wagner, Wuerfel, Infante-Duarte: "Iron oxide magnetic nanoparticles highlight early involvement of the choroid plexus in central nervous system inflammation." in: ASN neuro, Vol. 5, Issue 1, pp. e00110, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Augustin, Alsalih, Launey, Delbosc, Louedec, Ollivier, Chau, Montravers, Duval, Michel, Meilhac: "Predominant role of host proteases in myocardial damage associated with infectious endocarditis induced by Enterococcus faecalis in a rat model." in: Infection and immunity, Vol. 81, Issue 5, pp. 1721-9, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Fernández-López, Faustino, Derugin, Vexler: "Acute and chronic vascular responses to experimental focal arterial stroke in the neonate rat." in: Translational stroke research, Vol. 4, Issue 2, pp. 179-88, 2013 (PubMed).

Simon, Parlee, Learman, Mori, Scheller, Cawthorn, Ning, Gallagher, Tyrberg, Assadi-Porter, Evans, MacDougald: "Artificial sweeteners stimulate adipogenesis and suppress lipolysis independently of sweet taste receptors." in: The Journal of biological chemistry, Vol. 288, Issue 45, pp. 32475-89, 2013 (PubMed). Further details: Western Blotting

Ricard, Stanchi, Rodriguez, Amoureux, Rougon, Debarbieux: "Dynamic quantitative intravital imaging of glioblastoma progression reveals a lack of correlation between tumor growth and blood vessel density." in: PLoS ONE, Vol. 8, Issue 9, pp. e72655, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)