Laminin (LN) antibody

Details for Product No. ABIN268409
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Synonyms LAMC1, laminin
Human, Mouse (Murine), Rat (Rattus)
(93), (39), (22), (7), (6), (3), (2), (2), (2), (1), (1)
(79), (33), (12), (5)
(8), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(66), (66), (57), (37), (37), (23), (16), (14), (13), (13), (11), (9), (6), (3), (1), (1), (1)
Pubmed 2 references available
Quantity 25 μL
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Catalog No. ABIN268409
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Immunogen Laminin isolated from mouse EHS tumor.
Specificity This is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
Cross-Reactivity (Details) Rabbit reactivity reported in scientific literature (PMID: 18214989).
Purification IgG purified
Alternative Name Laminin
Background Laminin antibodies are widely used to label blood vessels and basement membranes.
Gene Symbol: LAMA1
Gene ID 284217
UniProt P19137
Research Area Neurology, Angiogenesis, Extracellular Matrix
Application Notes This Laminin antibody can be used for Immunocytochemistry/Immunofluorescence, Functional assays, Immunohistochemistry paraffin and frozen sections, and Western blotting where it detects bands at 200 and 400 kDa. The antibody functionally inhibits Laminin in mouse and rat. It binds to Laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue.
Recommended dilutions: Functional, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:500-1:2000, Immunohistochemistry-Frozen 1:500-1:2000, Immunohistochemistry-Paraffin 1:500-1:2000, Western Blot 1:100-1:5000
Protocol Immunohistochemistry-Paraffin protocol for Laminin Antibody Laminin Immunohistohemistry ABC/HRPThe fixation is routine paraformaldehyde or formalin fixation of tissue prior to paraffin embedding. However, the staining procedure must include an antigen retrieval step pretreating the deparaffinized sections with pepsin. This is required for all laminin antibodies used on paraffin tissue.Proteolytic antigen retrieval:Apply 250 µL of pepsin at 4mg/mL in 0.01M HCL (pH ~2.0).Incubate for 60 minutes at 37 degrees C in a humid chamber.Wash x2 in distilled H20, 5 min. each wash.Mount paraffin sections on Fisher Plus slides. Bake for >2 hours at 50C.
1. Deparaffinize mounted sections in xylene: 2 changes 5 min each, and a 3rd change for 10 min.
. Exchange solvent to ethanol with 2 changes of 100 % EtOH for 5 min each.
. Quench endogenous peroxidase for 30 min in 100 % methanol + 1 % H2O2.
. Hydrate to H2O in graded ethanol series. 5 min. in 95 % EtOH, 5 min. in 70 % EtOH, 5 min. in running H2O. Rinse with dH2O. Circumscribe the sections with PAP Pen.
. Unmask antigen by proteolysis. Cover sections with 100ul of 4mg/ml of pepsin (Sigma #P6887) dissolved in 0.01M HCl. Treat for 1 hr at 37C in humidified chamber. Rinse in running H2O.
. Blocking: Block background staining by covering sections with 100ul of PBS containing 10 % normal swine serum (Blocking
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General McDaniel, Rumer, Biroc et al.: "Remodeling of the mammary microenvironment after lactation promotes breast tumor cell metastasis." in: The American journal of pathology, Vol. 168, Issue 2, pp. 608-20, 2006 (PubMed).

Krekoski, Neubauer, Zuo et al.: "Axonal regeneration into acellular nerve grafts is enhanced by degradation of chondroitin sulfate proteoglycan." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 16, pp. 6206-13, 2001 (PubMed).

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