Bestrophin 1 antibody (BEST1) (C-Term)

Details for Product anti-BEST1 Antibody No. ABIN268414, Supplier: Log in to see
Antigen
  • CG6264
  • Dbest
  • Dmel\\CG6264
  • anon-WO0118547.380
  • best
  • dBest1
  • dbest1
  • dmBest1
  • BEST1
  • VMD2
  • best-1
  • ARB
  • BEST
  • BMD
  • RP50
  • TU15B
  • Bmd
  • Vmd2
  • mBest1
  • Bestrophin 1
  • bestrophin 1
  • Best1
  • BEST1
  • best1
  • Bm1_25500
Epitope
C-Term
4
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human
32
14
9
5
3
Host
Mouse
25
8
Clonality (Clone)
Monoclonal ()
Conjugate
This Bestrophin 1 antibody is un-conjugated
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
31
12
7
6
3
3
3
2
2
Options
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Immunogen Synthetic peptide conjugated to KLH corresponding to the C-terminus of human Bestrophin 1 (KDHMDPYWALENRDEAHS) [UniProt# O76090]
Clone E6-6
Isotype IgG1 kappa
Specificity Bestrophin (E6-6)
No Cross-Reactivity Rat (Rattus)
Purification Unpurified
Alternative Name Bestrophin 1 (BEST1 Antibody Abstract)
Background Gene Symbol: BEST1
Gene ID 7439
UniProt O76090
Application Notes Western Blot 1:1000, Immunocytochemistry/Immunofluorescence Assay dependent, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenBy Western blot, this antibody recognizes a band at ~68 kDa representing Bestrophin.** It is also useful for immunofluorescence. **Please see protocol for treatment of cell extract. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24345323)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for Bestrophin 1 Antibody Procedure Guide for NB 300-164 Monoclonal Anti-BestrophinWestern Blot Procedure
1. Run cell lysates** on an SDS-PAGE gel.
. Transfer the proteins to PVDF.
. Block the membrane in 1 % Carnation instant milk in PBS + 0.1 % Tween 20 (with 0.1mM CaCl2 and 1mM MgCl2) for 1 hour at RT.
. Dilute the anti-Bestrophin [NB 300-164] to 1:1,000 in 10 mL of fresh blocking buffer and incubate for 1 hour at RT.
. Wash the membrane with blocking buffer, 3x 5-10 minutes.
. Dilute the secondary antibody in fresh blocking buffer, as recommended by the secondary vendor and incubate for 1hour at RT.
. Wash the membrane with blocking buffer, 5x 8 minutes and rinse 1x with PBS (containing 0.1mM CaCl2 and 1mM MgCl2).
. Detect the protein-antibody complex with alkaline phosphatase, if using NBT/BCIP or with HRP, if using ECL.**Cell Lysate PreparationA. Lysates were prepared in lysis buffer [50mM Tris-HCl, pH 8 / 120mM NaCl / 0.5 % Nonidet P-40 / 10 µg/ml aprotinin / 10 µg/ml leupeptin / 1mM phenylmethylsulfonyl fluoride / 1mM sodium orthovanadate].B. Total protein content was determined by bicinchoninic acid assay (Pierce).
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Bestrophin 1 antibody (BEST1) (C-Term) (ABIN268414) Western blot detection of Bestrophin (68 kDa) from human RPE cell lysate using ABIN15...
Product cited in: Shim, Kim, Lee, Lee, Kwon, Song: "Survival of Transplanted Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells in a Human Recipient for 22 Months." in: JAMA ophthalmology, Vol. 135, Issue 3, pp. 287-290, 2017 (PubMed).

Uggenti, Briant, Streit, Thomson, Koay, Baines, Swanton, Manson: "Restoration of mutant bestrophin-1 expression, localisation and function in a polarised epithelial cell model." in: Disease models & mechanisms, Vol. 9, Issue 11, pp. 1317-1328, 2016 (PubMed). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Parker, Mitrousis, Shoichet: "Hydrogel for Simultaneous Tunable Growth Factor Delivery and Enhanced Viability of Encapsulated Cells in Vitro." in: Biomacromolecules, Vol. 17, Issue 2, pp. 476-84, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kaneko, Pappas, Tajiri, Borlongan: "Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro." in: Scientific reports, Vol. 6, pp. 35659, 2016 (PubMed).

Sharon, Al-Hamdani, Engelsberg, Mizrahi-Meissonnier, Obolensky, Banin, Sander, Jensen, Larsen, Schatz: "Ocular phenotype analysis of a family with biallelic mutations in the BEST1 gene." in: American journal of ophthalmology, Vol. 157, Issue 3, pp. 697-709.e1-2, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Bertolotti, Neri, Camparini, Macaluso, Marigo: "Stem cells as source for retinal pigment epithelium transplantation." in: Progress in retinal and eye research, Vol. 42, pp. 130-44, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Maruotti, Wahlin, Gorrell, Bhutto, Lutty, Zack: "A simple and scalable process for the differentiation of retinal pigment epithelium from human pluripotent stem cells." in: Stem cells translational medicine, Vol. 2, Issue 5, pp. 341-54, 2013 (PubMed).

Blenkinsop, Salero, Stern, Temple: "The culture and maintenance of functional retinal pigment epithelial monolayers from adult human eye." in: Methods in molecular biology (Clifton, N.J.), Vol. 945, pp. 45-65, 2012 (PubMed).

Krohne, Westenskow, Kurihara, Friedlander, Lehmann, Dorsey, Li, Zhu, Schultz, Wang, Siuzdak, Ding, Friedlander: "Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells." in: Stem cells translational medicine, Vol. 1, Issue 2, pp. 96-109, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Cho, Kim, Ku, Park, Lee, Yoo, Park, Song, Choi, Yu: "Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses." in: Stem cell research, Vol. 9, Issue 2, pp. 101-9, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Davidson, Millar, Burgess-Mullan, Maher, Urquhart, Brown, Black, Manson: "Functional characterization of bestrophin-1 missense mutations associated with autosomal recessive bestrophinopathy." in: Investigative ophthalmology & visual science, Vol. 52, Issue 6, pp. 3730-6, 2011 (PubMed).

Guziewicz, Slavik, Lindauer, Aguirre, Zangerl: "Molecular consequences of BEST1 gene mutations in canine multifocal retinopathy predict functional implications for human bestrophinopathies." in: Investigative ophthalmology & visual science, Vol. 52, Issue 7, pp. 4497-505, 2011 (PubMed). (Sample species: Dog (Canine)). Further details: Western Blotting

Harness, Turovets, Seiler, Nistor, Altun, Agapova, Ferguson, Laurent, Loring, Keirstead: "Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells." in: PLoS ONE, Vol. 6, Issue 1, pp. e14499, 2011 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Feng, Lu, Klimanskaya, Gomes, Kim, Chung, Honig, Kim, Lanza: "Hemangioblastic derivatives from human induced pluripotent stem cells exhibit limited expansion and early senescence." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 4, pp. 704-12, 2010 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Zhang, Patil, Marmorstein: "Bestrophin 2 is expressed in human non-pigmented ciliary epithelium but not retinal pigment epithelium." in: Molecular vision, Vol. 16, pp. 200-6, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

Lu, Malcuit, Wang, Girman, Francis, Lemieux, Lanza, Lund: "Long-term safety and function of RPE from human embryonic stem cells in preclinical models of macular degeneration." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 9, pp. 2126-35, 2009 (PubMed). (Sample species: Human). Further details: Western Blotting

Buchholz, Hikita, Rowland, Friedrich, Hinman, Johnson, Clegg: "Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 10, pp. 2427-34, 2009 (PubMed). (Sample species: Human). Further details: Western Blotting

Davidson, Millar, Urquhart, Burgess-Mullan, Shweikh, Parry, OSullivan, Maher, McKibbin, Downes, Lotery, Jacobson, Brown, Black, Manson: "Missense mutations in a retinal pigment epithelium protein, bestrophin-1, cause retinitis pigmentosa." in: American journal of human genetics, Vol. 85, Issue 5, pp. 581-92, 2009 (PubMed). (Sample species: Human).

Guziewicz, Zangerl, Lindauer, Mullins, Sandmeyer, Grahn, Stone, Acland, Aguirre: "Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease." in: Investigative ophthalmology & visual science, Vol. 48, Issue 5, pp. 1959-67, 2007 (PubMed). (Sample species: Dog (Canine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Klimanskaya, Hipp, Rezai, West, Atala, Lanza: "Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics." in: Cloning and stem cells, Vol. 6, Issue 3, pp. 217-45, 2005 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence