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Bestrophin 1 antibody (BEST1) (C-Term)

Details for Product anti-BEST1 Antibody No. ABIN268414, Supplier: Log in to see
Antigen
  • CG6264
  • Dbest
  • Dmel\\CG6264
  • anon-WO0118547.380
  • best
  • dBest1
  • dbest1
  • dmBest1
  • BEST1
  • VMD2
  • best-1
  • ARB
  • BEST
  • BMD
  • RP50
  • TU15B
  • Bmd
  • Vmd2
  • mBest1
Epitope
C-Term
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
28
13
9
5
2
Host
Mouse
22
7
Clonality (Clone)
Monoclonal ()
Conjugate
This Bestrophin 1 antibody is un-conjugated
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
27
12
8
4
3
2
Supplier
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Immunogen Synthetic peptide conjugated to KLH corresponding to the C-terminus of human Bestrophin 1 (KDHMDPYWALENRDEAHS)
Clone E6-6
Isotype IgG1 kappa
Specificity Bestrophin (E6-6)
No Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) Other species not tested.
Purification Ascites
Alternative Name Bestrophin-1 (BEST1 Antibody Abstract)
Background Best macular dystrophy (BMD) or vitelliform macular dystrophy (VMD2), is an autosomalform of macular degeneration, characterized by a depressed light peak in theelectrooculogram (EOG). It is inherited and has an early onset. Bestrophin is a 68 kDabasolateral plasma membrane protein encoded by the VMD2 gene. Bestrophin'sfunction is still unknown, but data suggests that it is a chloride channel that plays a rolein generating the altered EOG in Best disease patients. In addition, Bestrophin is auseful biochemical and histological marker of RPE (retinal pigment epithelial cells) cells.
Gene Symbol: BEST1
Gene ID 7439, 293735
UniProt O76090
Pathways
Application Notes By Western blot, this antibody recognizes a band at ~68 kDa representing Bestrophin.** It is also useful for immunofluorescence. **Please see protocol for treatment of cell extract. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24345323)
Recommended dilutions: Immunocytochemistry/Immunofluorescence Assay dependent, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:1000
Protocol Western Blot protocol specific for Bestrophin 1 Antibody Procedure Guide for NB 300-164 Monoclonal Anti-BestrophinWestern Blot Procedure
1. Run cell lysates** on an SDS-PAGE gel.
. Transfer the proteins to PVDF.
. Block the membrane in 1 % Carnation instant milk in PBS + 0.1 % Tween 20 (with 0.1mM CaCl2 and 1mM MgCl2) for 1 hour at RT.
. Dilute the anti-Bestrophin [NB 300-164] to 1:1,000 in 10 mL of fresh blocking buffer and incubate for 1 hour at RT.
. Wash the membrane with blocking buffer, 3x 5-10 minutes.
. Dilute the secondary antibody in fresh blocking buffer, as recommended by the secondary vendor and incubate for 1hour at RT.
. Wash the membrane with blocking buffer, 5x 8 minutes and rinse 1x with PBS (containing 0.1mM CaCl2 and 1mM MgCl2).
. Detect the protein-antibody complex with alkaline phosphatase, if using NBT/BCIP or with HRP, if using ECL.**Cell Lysate PreparationA. Lysates were prepared in lysis buffer [50mM Tris-HCl, pH 8 / 120mM NaCl / 0.5 % Nonidet P-40 / 10 µg/ml aprotinin / 10 µg/ml leupeptin / 1mM phenylmethylsulfonyl fluoride / 1mM sodium orthovanadate].B. Total protein content was determined by bicinchoninic acid assay (Pierce).
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Western Blotting (WB) image for anti-Bestrophin 1 antibody (BEST1) (C-Term) (ABIN268414) Western blot detection of Bestrophin (68 kDa) from human RPE cell lysate using ABIN15...
Product cited in: Klimanskaya, Hipp, Rezai et al.: "Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics." in: Cloning and stem cells, Vol. 6, Issue 3, pp. 217-45, 2005 (PubMed).

Marmorstein, Marmorstein, Rayborn et al.: "Bestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2), localizes to the basolateral plasma membrane of the retinal pigment epithelium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 23, pp. 12758-63, 2000 (PubMed).