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HIP1 antibody (Huntingtin Interacting Protein 1)

Details for Product anti-HIP1 Antibody No. ABIN268422, Supplier: Log in to see
Antigen
  • 2610109B09Rik
  • A930014B11Rik
  • CG10971
  • Dmel\\CG10971
  • E130315I21Rik
  • HIP-I
  • hip1
  • Hip1R
  • ILWEQ
  • MGC78813
  • mKIAA4113
Reactivity
Human
54
9
7
Host
Mouse
33
21
Clonality (Clone)
Monoclonal ()
Conjugate
This HIP1 antibody is un-conjugated
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
49
26
24
8
7
3
1
1
1
1
Supplier
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Immunogen Human 3'-HIP1 (~65 kDa)
Clone 4B10
Isotype IgG1 kappa
No Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) Does not react with rat.
Purification Ascites
Alternative Name HIP1 (HIP1 Antibody Abstract)
Background Huntingtin disease, a neurodegenerate disease, is caused by the expansion of apolymorphic glutamine tract in huntingtin. The Huntingtin Interacting Protein 1 (HIP-1) isa reportedly proapoptotic, cargo-specific adaptor protein that may be involved in thepathogenesis of Huntingtin disease. In addition to playing a role in Huntingtin disease, itis likely to be involved in the recruitment of clathrin coats to lipid membranes and it mayalso factor in tumorigenesis by allowing the survival of precancerous and cancerouscells. Since HIP-1 expression is significantly associated with prostate and colon cancermetastasis, HIP-1 can serve as a putative prognostic factor for prostate and coloncancers. Alternate Names: anti-HIP I antibody, anti-Huntingtin interacting protein 1 antibody.
Gene Symbol: HIP1
Gene ID 3092, 192154
Application Notes This HIP1 antibody is useful for Western blot, where a band at approximately 115 kDa is seen. Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation and Immunoprecipitation were reported in scientific literature.
Recommended dilutions: Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:10000
Protocol Protocol specific for HIP1 Antibody Western Blot Procedure
1. Run 25 µg/lane of protein (ie: HeLa whole cell extract) on a 10 % SDS-PAGE gel.
. Transfer the proteins to nitrocellulose.
. Block the membrane with a standard blocking buffer (5 % NFDM in TBST), overnight at 4 deg C.
. Dilute the anti-HIP1 in 1 % NFDM in TBST and incubate for 1hour at RT.
. Wash the membrane, 3x 5-10 minutes.
. Dilute the secondary antibody [anti-mouse IgG conjugated to HRP] in 1 % NFDM in TBST and incubate for 1hour at RT.
. Wash the membrane, 3x 5-10 minutes.
. Detect the protein-antibody complex via ECL.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Product cited in: Mills, Gaughan, Robson, Ross, McCracken, Kelly, Neal: "Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors." in: The Journal of cell biology, Vol. 170, Issue 2, pp. 191-200, 2005

Hyun, Rao, Saint-Dic, Michael, Kumar, Bradley, Mizukami, Oravecz-Wilson, Ross: "HIP1 and HIP1r stabilize receptor tyrosine kinases and bind 3-phosphoinositides via epsin N-terminal homology domains." in: The Journal of biological chemistry, Vol. 279, Issue 14, pp. 14294-306, 2004

Rao, Hyun, Kumar, Mizukami, Rubin, Lucas, Sanda, Ross: "Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival." in: The Journal of clinical investigation, Vol. 110, Issue 3, pp. 351-60, 2002

Background publications Rao, Chang, Kumar, Mizukami, Smithson, Bradley, Parlow, Ross: "Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors." in: Molecular and cellular biology, Vol. 21, Issue 22, pp. 7796-806, 2001