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HIP1 antibody (Huntingtin Interacting Protein 1)

Details for Product anti-HIP1 Antibody No. ABIN268423, Supplier: Log in to see
Antigen
  • 2610109B09Rik
  • A930014B11Rik
  • CG10971
  • Dmel\\CG10971
  • E130315I21Rik
  • HIP-I
  • hip1
  • Hip1R
  • ILWEQ
  • MGC78813
  • mKIAA4113
Reactivity
Bacteria, Human, Mouse (Murine)
58
12
8
1
Host
Mouse
35
23
Clonality (Clone)
Monoclonal ()
Conjugate
This HIP1 antibody is un-conjugated
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
53
26
26
11
9
7
4
1
1
1
1
Supplier
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Immunogen Human 3'-HIP1 (~65 kDa)
Clone 1B11
Isotype IgG1 kappa
Specificity This antibody is specific for human HIP1. Does not cross-react with HIP1r.
No Cross-Reactivity Rat (Rattus)
Purification Ascites
Alternative Name HIP1 (HIP1 Antibody Abstract)
Background Gene Symbol: HIP1
Molecular Weight Theoretical MW: 115 kDa
Gene ID 3092, 192154
UniProt O00291
Application Notes Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for HIP1 Antibody Western Blot Procedure
1. Run 25 µg/lane of protein (ie: HeLa whole cell extract) on a 10 % SDS-PAGE gel.
. Transfer the proteins to nitrocellulose.
. Block the membrane with a standard blocking buffer (5 % NFDM in TBST), overnight at 4 deg C.
. Dilute the anti-HIP1 in 1 % NFDM in TBST and incubate for 1hour at RT.
. Wash the membrane, 3x 5-10 minutes.
. Dilute the secondary antibody [anti-mouse IgG conjugated to HRP] in 1 % NFDM in TBST and incubate for 1hour at RT.
. Wash the membrane, 3x 5-10 minutes.
. Detect the protein-antibody complex via ECL.
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-HIP1 antibody (Huntingtin Interacting Protein 1) (ABIN268423) Western Blot: HIP1 Antibody (1B11) [ABIN152545] - HeLa whole cell extracts.
Product cited in: Choudhury, Raychaudhuri, Bhattacharyya: "Identification of HYPK-interacting proteins reveals involvement of HYPK in regulating cell growth, cell cycle, unfolded protein response and cell death." in: PLoS ONE, Vol. 7, Issue 12, pp. e51415, 2012 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Datta, Bhattacharyya: "Regulation of RE1 protein silencing transcription factor (REST) expression by HIP1 protein interactor (HIPPI)." in: The Journal of biological chemistry, Vol. 286, Issue 39, pp. 33759-69, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Datta, Choudhury, Lahiri, Bhattacharyya: "Genome wide gene expression regulation by HIP1 Protein Interactor, HIPPI: prediction and validation." in: BMC genomics, Vol. 12, pp. 463, 2011 (PubMed).

Banerjee, Datta, Majumder, Mukhopadhyay, Bhattacharyya: "Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1." in: Nucleic acids research, Vol. 38, Issue 3, pp. 878-92, 2010 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Mills, Gaughan, Robson, Ross, McCracken, Kelly, Neal: "Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors." in: The Journal of cell biology, Vol. 170, Issue 2, pp. 191-200, 2005 (PubMed). (Sample species: Bacteria). Further details: Western Blotting

Rao, Hyun, Kumar, Mizukami, Rubin, Lucas, Sanda, Ross: "Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival." in: The Journal of clinical investigation, Vol. 110, Issue 3, pp. 351-60, 2002 (PubMed). Further details: Western Blotting

Rao, Chang, Kumar, Mizukami, Smithson, Bradley, Parlow, Ross: "Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors." in: Molecular and cellular biology, Vol. 21, Issue 22, pp. 7796-806, 2001 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence