Huntingtin Interacting Protein 1 (HIP1) antibody

Details for Product No. ABIN268423
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CG10971, Dmel\\CG10971, Hip1R, hip1, MGC78813, HIP-I, ILWEQ, 2610109B09Rik, A930014B11Rik, E130315I21Rik, mKIAA4113
Reactivity
Human, Mouse (Murine)
(55), (17), (15), (12), (12)
Host
Mouse
(33), (24)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(45), (19), (12), (10), (9), (4), (3), (1), (1), (1)
Pubmed 2 references available
Quantity 0.025 mL
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN268423
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human 3'-HIP1 (~65 kDa)
Clone 1B11
Isotype IgG1 kappa
Specificity NB300-204 is specific for human HIP1. Does not cross-react with HIP1r.
No Cross-Reactivity Rat (Rattus)
Purification Ascites
Alternative Name HIP1
Background Huntingtin disease, a neurodegenerate disease, is caused by the expansion of apolymorphic glutamine tract in huntingtin. The Huntingtin Interacting Protein 1 (HIP-1) isa reportedly proapoptotic, cargo-specific adaptor protein that may be involved in thepathogenesis of Huntingtin disease. As well as playing a role in Huntingtin disease, it islikely to be involved in the recruitment of clathrin coats to lipid membranes and it mayalso factor in tumorigenesis by allowing the survival of precancerous and cancerouscells. Since HIP-1 expression is significantly associated with prostate and colon cancermetastasis, HIP-1 can serve as a putative prognostic factor for prostate and coloncancers. Alternate Names: anti-HIP I antibody, anti-Huntingtin interacting protein 1 antibody.
Gene Symbol: HIP1
Gene ID 3092
Application Notes This HIP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is observed at ~115 kDa. Use in Immunoprecipitation reported in scientific literature (PMID 23272104)
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation, Western Blot 1:10000
Protocol Protocol specific for HIP1 Antibody Western Blot Procedure
1. Run 25 µg/lane of protein (ie: HeLa whole cell extract) on a 10 % SDS-PAGE gel.
. Transfer the proteins to nitrocellulose.
. Block the membrane with a standard blocking buffer (5 % NFDM in TBST), overnight at 4 deg C.
. Dilute the anti-HIP1 in 1 % NFDM in TBST and incubate for 1hour at RT.
. Wash the membrane, 3x 5-10 minutes.
. Dilute the secondary antibody [anti-mouse IgG conjugated to HRP] in 1 % NFDM in TBST and incubate for 1hour at RT.
. Wash the membrane, 3x 5-10 minutes.
. Detect the protein-antibody complex via ECL.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
General Rao, Chang, Kumar et al.: "Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors." in: Molecular and cellular biology, Vol. 21, Issue 22, pp. 7796-806, 2001 (PubMed).

Rao, Hyun, Kumar et al.: "Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival." in: The Journal of clinical investigation, Vol. 110, Issue 3, pp. 351-60, 2002 (PubMed).

Validation Images
Did you look for something else?
back to top