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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase)

Details for Product anti-GAPDH Antibody No. ABIN268426, Supplier: Log in to see
Antigen
  • CG8893
  • Dmel\\CG8893
  • GA3PDH
  • GADPH
  • GAP
  • GAPDH
  • GAPDH II
  • GAPDH2
  • Gapd
  • Gapdh
  • Gapdh-2
  • Gapdh13F
  • GAPD
  • g3pd
  • gapd
  • GB14798
  • GAPC-2
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • T6J4.17
  • T6J4_17
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • BEST:GH12586
  • CG12055
  • Dmel\\CG12055
  • GAPDH I
  • GAPDH-1
  • GAPDH1
  • GAPDHI
  • Gapdh-1
  • Gapdh43E
  • gadph
  • gapdh
  • gapdh-1
  • gh12586
  • KNC-NDS6
  • G3PD
  • cb609
  • mg:bb02e05
  • wu:fb33a10
  • wu:ft80f05
  • G3PDH
  • Glyceraldehyde 3 phosphate dehydrogenase 2
  • glyceraldehyde-3-phosphate dehydrogenase
  • glyceraldehyde 3-phosphate dehydrogenase
  • similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  • glyceraldehyde-3-phosphate dehydrogenase, type I
  • Glyceraldehyde 3 phosphate dehydrogenase 1
  • glyceraldehyde-3-phosphate dehydrogenase-like
  • olfactory receptor, family 8, subfamily K, member 3-like
  • Gapdh2
  • GAPDH
  • gapdh
  • Gapdh
  • GAPC2
  • gapDH
  • Gapdh1
  • LOC614985
Reactivity
Bird (Avian), Chicken, Cow (Bovine), Hamster, Horse (Equine), Human, Mouse (Murine), Opossum (Didelphimorphia), Pig (Porcine), Primate, Rat (Rattus), Sheep (Ovine), Zebrafish
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This GAPDH antibody is un-conjugated
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Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
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Options
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Available images

Immunogen Purified porcine GAPDH [UniProt# P00355]
Clone 1D4
Isotype IgM kappa
Purification Immunogen affinity purified
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Gene Symbol: GAPDH
Molecular Weight Theoretical MW: 38 kDa
Gene ID 2597
UniProt P00355
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes Western Blot 1:1000, Simple Western 1:25, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)This GAPDH Antibody (1D4) is useful for Western blot and Immunocytochemistry/Immunofluorescence. Immunohistochemistry was reported in scientific literature. In WB a prominent band can be seen at approximately 38 kDa. This antibody makes an excellent loading control for Western blot experiments. Use in immunprecipitation reported in scientific literature (PMID 24032460). Use in chromatin immunoprecipitation reported in scientific literature (PMID 25802280). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 10 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN268426) Sciatic nerves of mouse wild type (+/+), heterozygous (+/o) and homozygous (o/o) for ...
Immunofluorescence (IF) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN268426) Human neuroblastma line Sh-SY5Y stained with anti-GAPDH (ABIN152561) [green] and an a...
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN268426) Western Blot: GAPDH Antibody (1D4) [ABIN152561] - analysis of GAPDH in human nucleus ...
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN268426) Western Blot: GAPDH Antibody (1D4) [ABIN152561] - Zebrafish embryo lysates at differe...
Immunofluorescence (IF) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN268426) Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [ABIN152561] - SH-SY5Y c...
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN268426) Western Blot: GAPDH Antibody (1D4) [ABIN152561] - analysis of GAPDH in chicken pector...
Simple Western (SimWes) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN268426) Simple Western: GAPDH Antibody (1D4) [ABIN152561] - Simple Western lane view shows a ...
Product cited in: Abou-Kheir, Barrak, Hadadeh, Daoud: "HTR-8/SVneo cell line contains a mixed population of cells." in: Placenta, Vol. 50, pp. 1-7, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Barr, Cooper, Heldt, Butera, Stoy, Mansfeld, Novák, Bakal: "DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression." in: Nature communications, Vol. 8, pp. 14728, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sero, Bakal: "Multiparametric Analysis of Cell Shape Demonstrates that β-PIX Directly Couples YAP Activation to Extracellular Matrix Adhesion." in: Cell systems, Vol. 4, Issue 1, pp. 84-96.e6, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Lassance, Miedl, Konya, Heinemann, Ebner, Hackl, Desoye, Hiden: "Differential response of arterial and venous endothelial cells to extracellular matrix is modulated by oxygen." in: Histochemistry and cell biology, Vol. 137, Issue 5, pp. 641-55, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Kokubun, Pankajakshan, Kim, Agrawal: "Differentiation of porcine mesenchymal stem cells into epithelial cells as a potential therapeutic application to facilitate epithelial regeneration." in: Journal of tissue engineering and regenerative medicine, Vol. 10, Issue 2, pp. E73-83, 2016 (PubMed). (Sample species: Pig (Porcine)).

Serbo, Kuo, Lewis, Lehmann, Li, Gracias, Romer: "Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton." in: Advanced healthcare materials, Vol. 5, Issue 1, pp. 146-58, 2016 (PubMed).

Becs, Zarjou, Agarwal, Kovács, Becs, Nyitrai, Balogh, Bányai, Eaton, Arosio, Poli, Jeney, Balla, Balla: "Pharmacological induction of ferritin prevents osteoblastic transformation of smooth muscle cells." in: Journal of cellular and molecular medicine, Vol. 20, Issue 2, pp. 217-30, 2016 (PubMed).

Slocum, Skoko, Wakabayashi, Aja, Yamamoto, Kensler, Chartoumpekis: "Keap1/Nrf2 pathway activation leads to a repressed hepatic gluconeogenic and lipogenic program in mice on a high-fat diet." in: Archives of biochemistry and biophysics, Vol. 591, pp. 57-65, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Chen, Statt, Wu, Chang, Liao, Wang, Shyu, Lee: "High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells." in: Scientific reports, Vol. 6, pp. 18815, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Kümper, Mardakheh, McCarthy, Yeo, Stamp, Paul, Worboys, Sadok, Jørgensen, Guichard, Marshall: "Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis." in: eLife, Vol. 5, pp. e12994, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Krapp, Hotter, Gawanbacht, McLaren, Kluge, Stürzel, Mack, Reith, Engelhart, Ciuffi, Hornung, Sauter, Telenti, Kirchhoff: "Guanylate Binding Protein (GBP) 5 Is an Interferon-Inducible Inhibitor of HIV-1 Infectivity." in: Cell host & microbe, Vol. 19, Issue 4, pp. 504-14, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Ochaba, Monteys, ORourke, Reidling, Steffan, Davidson, Thompson: "PIAS1 Regulates Mutant Huntingtin Accumulation and Huntington's Disease-Associated Phenotypes In Vivo." in: Neuron, Vol. 90, Issue 3, pp. 507-20, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Drelon, Berthon, Sahut-Barnola, Mathieu, Dumontet, Rodriguez, Batisse-Lignier, Tabbal, Tauveron, Lefrançois-Martinez, Pointud, Gomez-Sanchez, Vainio, Shan, Sacco, Schedl, Stratakis, Martinez, Val: "PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development." in: Nature communications, Vol. 7, pp. 12751, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Ding, Paszkowski-Rogacz, Winzi, Chakraborty, Theis, Singh, Ciotta, Poser, Roguev, Chu, Choudhary, Mann, Stewart, Krogan, Buchholz: "Systems Analyses Reveal Shared and Diverse Attributes of Oct4 Regulation in Pluripotent Cells." in: Cell systems, Vol. 1, Issue 2, pp. 141-51, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Willi, Yoo, Wang, Trajanoski, Hennighausen: "Differential cytokine sensitivities of STAT5-dependent enhancers rely on Stat5 autoregulation." in: Nucleic acids research, Vol. 44, Issue 21, pp. 10277-10291, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Sandin, Bergkvist, Nath, Kielkopf, Janefjord, Helmfors, Zetterberg, Blennow, Li, Nilsberth, Garner, Brorsson, Kågedal: "Beneficial effects of increased lysozyme levels in Alzheimer's disease modelled in Drosophila melanogaster." in: The FEBS journal, Vol. 283, Issue 19, pp. 3508-3522, 2016 (PubMed).

Yoon, Storm, Kamimae-Lanning, Goloviznina, Kurre: "Endogenous DNA Damage Leads to p53-Independent Deficits in Replicative Fitness in Fetal Murine Fancd2(-/-) Hematopoietic Stem and Progenitor Cells." in: Stem cell reports, Vol. 7, Issue 5, pp. 840-853, 2016 (PubMed).

Sankowski, Herring, Keyvani, Frenzel, Wu, Röskam, Noelker, Bacher, Al-Abed: "The multi-target effects of CNI-1493: convergence of anti-amylodogenic and anti-inflammatory properties in animal models of Alzheimer's disease." in: Molecular medicine (Cambridge, Mass.), Vol. 22, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Zhao, Koenig, Trask, Lin, Hans, Garg, Lilly: "MicroRNA miR145 regulates TGFBR2 expression and matrix synthesis in vascular smooth muscle cells." in: Circulation research, Vol. 116, Issue 1, pp. 23-34, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Ye, Zhou, Markova, Tian, Li, Anderson, Shapiro, Risbud: "Xylosyltransferase-1 expression is refractory to inhibition by the inflammatory cytokines tumor necrosis factor α and IL-1β in nucleus pulposus cells: novel regulation by AP-1, Sp1, and Sp3." in: The American journal of pathology, Vol. 185, Issue 2, pp. 485-95, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting