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Details for Product No. ABIN268426

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody

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Antigen
Synonyms
G3PD, GAPD, Gapd, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, G3PDH, CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh, Gapdh-2, Gapdh13F, BEST:GH12586, CG12055,  ... show more
Reactivity
»Alternatives Bird (Avian)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunofluorescence (IF), Western Blotting (WB)
Pubmed 14 references available
Catalog no. ABIN268426
Quantity 0.1 mL
Price
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Immunogen The immunogen used to raise this particular antibody was extensively purified pigGAPDH.
Clone 1D4
Isotype IgG1
Specificity Species Reactivity: ABIN152561 reacts with GAPDH from human, cow, pig, mouse, rat and bird. SinceGAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also.
Cross-Reactivity Bird (Avian), Human, Mouse (Murine), Rat (Rattus)
Alternative Name GAPDH
Background GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible forcatalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation ofglyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amountsand which is required at all times for an important house keeping functions, levels ofGAPDH mRNA are often used as standards in studies of mRNA expression.Increasingly, scientists are making use of specific antibodies to GAPDH as loadingcontrols for western blotting experiments. Apart from a role in glycolysis, GAPDH mayhave other roles such as in the activation of transcription (1). GAPDH is reported to bindto a variety of other proteins, including the amyloid precursor protein, mutations in whichcause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin,the protein product aberrant forms of which are causative of Huntington's disease (2,3).Associations with actin and tubulin have also be reported. The protein may also have arole in the regulation of apoptosis, and interestingly migrates from the cytoplasm into thenucleus when cells become apoptotic (4). Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Gene ID 2597
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes ABIN152561 can be used in Western blot and immunocytochemical experiments. Onblots ABIN152561 reveals a prominent ~37kDa band, and on cells in tissue culture theantibody stains in a punctate cytoplasmic. Suggested working dilutions: *Western Blot - 1:1,000 (ECL)Immunofluorescence - 1:100*The investigator should determine the optimalworking dilution for a specific application.
Restrictions For Research Use only
Concentration 1 mg/ml
Storage 4 °C
Kapadia, Brideau-Andersen, Chisari: "Interference of hepatitis C virus RNA replication by short interfering RNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 4, pp. 2014-8, 2003 (PubMed).

Akassoglou, Malester, Xu et al.: "Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 14, pp. 5075-80, 2004 (PubMed).

Moshynska, Sankaran, Pahwa et al.: "Prognostic significance of a short sequence insertion in the MCL-1 promoter in chronic lymphocytic leukemia." in: Journal of the National Cancer Institute, Vol. 96, Issue 9, pp. 673-82, 2004 (PubMed).

Sarkar, Chawla-Sarkar, Young et al.: "Myocardial cell death and regeneration during progression of cardiac hypertrophy to heart failure." in: The Journal of biological chemistry, Vol. 279, Issue 50, pp. 52630-42, 2004 (PubMed).

Godsel, Hsieh, Amargo et al.: "Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin." in: The Journal of cell biology, Vol. 171, Issue 6, pp. 1045-59, 2005 (PubMed).

Pisitkun, Bieniek, Tchapyjnikov et al.: "High-throughput identification of IMCD proteins using LC-MS/MS." in: Physiological genomics, Vol. 25, Issue 2, pp. 263-76, 2006 (PubMed).

Barry, Davidsen, Limesand et al.: "Developmental changes in ovine myocardial glucose transporters and insulin signaling following hyperthermia-induced intrauterine fetal growth restriction." in: Experimental biology and medicine (Maywood, N.J.), Vol. 231, Issue 5, pp. 566-75, 2006 (PubMed).

Ju, Gitcho, Casmaer et al.: "Potentiation of insulin-stimulated glucose transport by the AMP-activated protein kinase." in: American journal of physiology. Cell physiology, Vol. 292, Issue 1, pp. C564-72, 2007 (PubMed).

Woeller, Fox, Perry et al.: "A ferritin-responsive internal ribosome entry site regulates folate metabolism." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 29927-35, 2007 (PubMed).

Cui, Cuartas, Ke et al.: "CD200 and its receptor, CD200R, modulate bone mass via the differentiation of osteoclasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14436-41, 2007 (PubMed).

Limesand, Rozance, Smith et al.: "Increased insulin sensitivity and maintenance of glucose utilization rates in fetal sheep with placental insufficiency and intrauterine growth restriction." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 6, pp. E1716-25, 2007 (PubMed).

Chen, Tu, Zhang et al.: "PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells." in: The Journal of biological chemistry, Vol. 283, Issue 5, pp. 2508-17, 2008 (PubMed).

Zhang, Eaton, Snyder et al.: "Tempol protects against oxidative damage and delays epithelial tumor onset in Fanconi anemia mice." in: Cancer research, Vol. 68, Issue 5, pp. 1601-8, 2008 (PubMed).

Diaz Encarnacion, Warner, Gray et al.: "Signaling pathways modulated by fish oil in salt-sensitive hypertension." in: American journal of physiology. Renal physiology, Vol. 294, Issue 6, pp. F1323-35, 2008 (PubMed).

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts (251), (199), (37), (22), (19)
Reactivities (396), (246), (211), (108), (59), (39), (38), (36), (27), (26), (24), (20), (16), (14), (12), (11), (10), (9), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (446), (238), (139), (81), (79), (60), (50), (37), (17), (13), (7), (6), (5), (5), (4), (2), (2), (1), (1)
Conjugates (30), (24), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1)
Epitopes (79), (36), (13), (10), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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