anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase)

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CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, gl ... show more
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, glyceraldehyde-3-phosphate dehydrogenase C2, BEST:GH12586, CG12055, Dmel\\CG12055, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, KNC-NDS6, G3PD, cb609, mg:bb02e05, wu:fb33a10, wu:ft80f05, G3PDH show less
Bird (Avian)
(568), (229), (198), (118), (61), (50), (45), (38), (28), (25), (21), (19), (18), (17), (12), (12), (10), (9), (9), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(342), (250), (62), (31), (21)
Clonality (Clone)
Monoclonal ()
This GAPDH antibody is un-conjugated
(43), (40), (18), (6), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
(618), (288), (161), (127), (92), (69), (47), (31), (28), (17), (10), (9), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 14 references available
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Quantity 0.1 mL
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Immunogen Purified porcine GAPDH
Clone 1D4
Isotype IgM kappa
Cross-Reactivity (Details) Likely to react with other mammals as well. Hamster,zebrafish reactivity reported by a customer review. chicken reactivity reported in scientific literature (PMID: 24725420)
Purification Tissue culture supernatant
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible forcatalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation ofglyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amountsand which is required at all times for an important house keeping functions, levels ofGAPDH mRNA are often used as standards in studies of mRNA expression.Increasingly, scientists are making use of specific antibodies to GAPDH as loadingcontrols for western blotting experiments. Apart from a role in glycolysis, GAPDH mayhave other roles such as in the activation of transcription (1). GAPDH is reported to bindto a variety of other proteins, including the amyloid precursor protein, mutations in whichcause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin,the protein product aberrant forms of which are causative of Huntington's disease (2,3).Associations with actin and tubulin have also be reported. The protein may also have arole in the regulation of apoptosis, and interestingly migrates from the cytoplasm into thenucleus when cells become apoptotic (4). Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Gene Symbol: GAPDH
Gene ID 2597
UniProt P00355
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes This GAPDH Antibody (1D4) is useful for Western blot and Immunocytochemistry/Immunofluorescence. Immunohistochemistry was reported in scientific literature. In WB a prominent band can be seen at approximately 38 kDa. This antibody makes an excellent loading control for Western blot experiments.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry, Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Product cited in: Diaz Encarnacion, Warner, Gray et al.: "Signaling pathways modulated by fish oil in salt-sensitive hypertension." in: American journal of physiology. Renal physiology, Vol. 294, Issue 6, pp. F1323-35, 2008 (PubMed).

Zhang, Eaton, Snyder et al.: "Tempol protects against oxidative damage and delays epithelial tumor onset in Fanconi anemia mice." in: Cancer research, Vol. 68, Issue 5, pp. 1601-8, 2008 (PubMed).

Chen, Tu, Zhang et al.: "PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells." in: The Journal of biological chemistry, Vol. 283, Issue 5, pp. 2508-17, 2008 (PubMed).

Limesand, Rozance, Smith et al.: "Increased insulin sensitivity and maintenance of glucose utilization rates in fetal sheep with placental insufficiency and intrauterine growth restriction." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 6, pp. E1716-25, 2007 (PubMed).

Cui, Cuartas, Ke et al.: "CD200 and its receptor, CD200R, modulate bone mass via the differentiation of osteoclasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14436-41, 2007 (PubMed).

Woeller, Fox, Perry et al.: "A ferritin-responsive internal ribosome entry site regulates folate metabolism." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 29927-35, 2007 (PubMed).

Ju, Gitcho, Casmaer et al.: "Potentiation of insulin-stimulated glucose transport by the AMP-activated protein kinase." in: American journal of physiology. Cell physiology, Vol. 292, Issue 1, pp. C564-72, 2007 (PubMed).

Barry, Davidsen, Limesand et al.: "Developmental changes in ovine myocardial glucose transporters and insulin signaling following hyperthermia-induced intrauterine fetal growth restriction." in: Experimental biology and medicine (Maywood, N.J.), Vol. 231, Issue 5, pp. 566-75, 2006 (PubMed).

Pisitkun, Bieniek, Tchapyjnikov et al.: "High-throughput identification of IMCD proteins using LC-MS/MS." in: Physiological genomics, Vol. 25, Issue 2, pp. 263-76, 2006 (PubMed).

Godsel, Hsieh, Amargo et al.: "Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin." in: The Journal of cell biology, Vol. 171, Issue 6, pp. 1045-59, 2005 (PubMed).

Sarkar, Chawla-Sarkar, Young et al.: "Myocardial cell death and regeneration during progression of cardiac hypertrophy to heart failure." in: The Journal of biological chemistry, Vol. 279, Issue 50, pp. 52630-42, 2004 (PubMed).

Moshynska, Sankaran, Pahwa et al.: "Prognostic significance of a short sequence insertion in the MCL-1 promoter in chronic lymphocytic leukemia." in: Journal of the National Cancer Institute, Vol. 96, Issue 9, pp. 673-82, 2004 (PubMed).

Akassoglou, Malester, Xu et al.: "Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 14, pp. 5075-80, 2004 (PubMed).

Kapadia, Brideau-Andersen, Chisari: "Interference of hepatitis C virus RNA replication by short interfering RNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 4, pp. 2014-8, 2003 (PubMed).

Catalog No. ABIN268426
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