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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
Alternatives Immunofluorescence (IF), Western Blotting (WB)
|14 references available|
|Quantity||0.1 ml (1 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||The immunogen used to raise this particular antibody was extensively purified pigGAPDH.|
|Cross-Reactivity||Bird (Avian), Human, Mouse (Murine), Rat (Rattus)|
|Description||GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible forcatalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation ofglyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amountsand which is required at all times for an important house keeping functions, levels ofGAPDH mRNA are often used as standards in studies of mRNA expression.Increasingly, scientists are making use of specific antibodies to GAPDH as loadingcontrols for western blotting experiments. Apart from a role in glycolysis, GAPDH mayhave other roles such as in the activation of transcription (1). GAPDH is reported to bindto a variety of other proteins, including the amyloid precursor protein, mutations in whichcause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin,the protein product aberrant forms of which are causative of Huntington's disease (2,3).Associations with actin and tubulin have also be reported. The protein may also have arole in the regulation of apoptosis, and interestingly migrates from the cytoplasm into thenucleus when cells become apoptotic (4). Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.|
|Specificity||Species Reactivity: ABIN152561 reacts with GAPDH from human, cow, pig, mouse, rat and bird. SinceGAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also.|
|Synonyms||G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17|
|Application Notes||ABIN152561 can be used in Western blot and immunocytochemical experiments. Onblots ABIN152561 reveals a prominent ~37kDa band, and on cells in tissue culture theantibody stains in a punctate cytoplasmic. Suggested working dilutions: *Western Blot - 1:1,000 (ECL)Immunofluorescence - 1:100*The investigator should determine the optimalworking dilution for a specific application.|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Research Area||Cancer, Metabolism, Alzheimer's Disease, Enzymes|
|Restrictions||For Research Use only|
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