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Vimentin (VIM) antibody

Antigen

Vimentin (VIM)
Synonyms FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Clonality Polyclonal
Host
Alternatives

Chicken
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
3 references available
Catalog no. ABIN268427
Quantity 25 µl  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name Vimentin
Gene ID 7431
Immunogen The immunogen used to generate our antibody was recombinant vimentin purified fromE. coli.
Description Vimentin is the 10nm or intermediate filament protein subunit found in many kinds ofmesenchymal and epithelia cells, in many kinds of cells in tissue culture and indeveloping neuronal and astrocytic precursor cells in the central nervous system.Vimentin frequently forms copolymers with other intermediate filament proteins, such asGFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilamentproteins (in developing neurons). Antibodies to vimentin are useful in studies of stemcells and generally to reveal the filamentous cytoskeleton. Alternate Names: anti-FLJ36605 antibody, anti-VIM antibody.
Specificity Species Reactivity: ABIN152563 reacts with Vimentin from human, mouse, and rat.

Application Details
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Application Notes Suggested working dilutions: immunofluorescence 1:5000,Immunohistochemistry-Paraffin 1:150
Buffer Preservative: 5mM Sodium Azide.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Rankin, Tomaszewski, Haase: "Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor." in: Cancer research, Vol. 66, Issue 5, pp. 2576-83, 2006 (PubMed).

Okawa, Michaylira, Kalabis et al.: "The functional interplay between EGFR overexpression, hTERT activation, and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, and differentiation." in: Genes & development, Vol. 21, Issue 21, pp. 2788-803, 2007 (PubMed).

Johnstone, Mongroo, Rich et al.: "Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation." in: Molecular and cellular biology, Vol. 28, Issue 2, pp. 687-704, 2008 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts Mouse (169), Rabbit (160), Chicken (17), Sheep (6), Guinea Pig (4), Rat (4), Goat (3)
Reactivities Human (305), Rat (Rattus) (131), Mouse (Murine) (120), Cow (Bovine) (77), Pig (Porcine) (73), Dog (Canine) (52), Chicken (48), Sheep (Ovine) (37), Guinea Pig (18), Horse (Equine) (18), Monkey (13), Goat (12), Mammalian (12), Hamster (10), Zebrafish (Danio rerio) (6), Amphibian (4), Xenopus laevis (3), Zebrafish (3), Potoroo (2), All Species (1), Cat (Feline) (1), Fish (1), Frog (1), Primate (1), Rabbit (1)
Applications Western Blotting (WB) (232), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (117), ELISA (116), Immunofluorescence (IF) (103), Immunocytochemistry (ICC) (79), Immunohistochemistry (IHC) (74), Flow Cytometry (FACS) (36), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunoprecipitation (IP) (25), Immunohistochemistry (Fixed) (IHC (fx)) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunoelectron Microscopy (IEM) (3), Immunoelectrophoresis (IEP) (3), Intracellular Staining (IS) (3), To be determined by user (TBD) (3), Dot Blot (Dot) (1), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)) (1), Transfected cell culture (TCC) (1)
Conjugates FITC (13), Biotin (12), PE (8), Alexa Fluor 488 (7), HRP (7), Dyomics 547 (4), Alexa Fluor 350 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), DY547 (2), DyLight 549 (2), APC (1)
Epitopes C-Term (23), pSer82 (23), pSer56 (19), pSer72 (13), N-Term (11), AA 210-466 (8), Internal Region (6), pSer38,N-Term (3), pSer72,Internal Region (3), pTyr61,N-Term (3), Center (2), Ser82 (2), pSer38 (2), AA 2-466 (1), AA 300-350 (1), AA 400-500 (1), AA 70-120 (1), pSer33 (1), pSer50 (1), pSer55 (1), pSer6 (1), pSer71 (1), pSer83 (1)