His Tag antibody

Details for Product No. ABIN2688142, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
620
18
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3
1
Host
Mouse
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181
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23
4
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3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
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45
36
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2
2
2
1
1
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1
1
1
1
1
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1
Application
Dot Blot (DB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
545
386
163
105
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68
26
12
11
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1
1
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Options
Supplier
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Immunogen 6x His synthetic peptide.
Sequence HHHHHH
Clone HIS-H8
Isotype IgG2b
Specificity His-tag fusion protein
Purification IgG fraction
Target Type Tag
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Application Notes ICC/IHC/IF/IP 1/200. WB 1/1000. Other applications are optimized by end users.
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer PBS, pH 7.4 with 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Product cited in: Wyroba, Kwaśniak, Miller, Kobyłecki, Osińska: "Site-directed mutagenesis, in vivo electroporation and mass spectrometry in search for determinants of the subcellular targeting of Rab7b paralogue in the model eukaryote Paramecium octaurelia." in: European journal of histochemistry : EJH, Vol. 60, Issue 2, pp. 2612, 2016 (PubMed). Method employed by authors: Confocal Microscopy (CM)

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