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SCARB2 antibody (Scavenger Receptor Class B, Member 2) (N-Term)

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Synonyms cd36l2, fi13c07, chunp6914, wu:fi13c07, SCARB2, scarb2, AMRF, CD36L2, EPM4, HLGP85, LGP85, LIMP-2, LIMPII, SR-BII, 9330185J12Rik, Cd36l2, MLGP85, LimpII
N-Term, AA 400-478
(18), (13), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Cow (Bovine), Hamster, Human, Mouse (Murine), Rat (Rattus), Mustelid
(54), (31), (10), (3), (2), (2), (1), (1), (1)
(43), (17), (6), (1)
This SCARB2 antibody is un-conjugated
(2), (2), (2), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(54), (39), (21), (15), (5), (5), (4), (4), (3), (1)
Pubmed 8 references available
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Quantity 25 μL
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Immunogen A peptide containing residues from mouse SR-BII (between residues 400-478) plus an N-terminal cysteine was coupled to KLH.
Purification Whole antisera
Alternative Name SCARB2 (SCARB2 Antibody Abstract)
Background High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and theirplasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI andSR-BII (previously known as SR-BI.2) are the alternatively spliced products of a singlegene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain isalmost completely different. SR-BII binds HDLs and mediates selective uptake of HDLcholesteryl ester, but with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressingSR-BI, with SR-BII expression found in liver, adrenal glands, and testes.Although the role of SR-BII is not completely clear, research suggests that it may be afunctional HDL receptor. In addition, SR-BII mRNA results from the alternative splicingof SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer oflipid between HDL and cells. The relative expression and functional activities of thesetwo isoforms create a potential means of regulating selective lipid transfer between HDLand cells. (1-6). Alternate Names: anti-SR-B2 antibody, anti-SR-BII antibody, anti-Scavenger Receptor-BII antibody,anti-Scavenger Receptor-B2 antibody. Related Diseases: Cancer
Gene Symbol: SCARB2
Gene ID 950, 12492, 117106
UniProt O35114
Application Notes This SR-BII antibody is useful for Immunohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at ~82 kDa in tissues that express SR-BII such as liver, testes, and adrenal glands. Flow Cytometry was reported in scientific literature.
Recommended dilutions: Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:25-1:100, Immunohistochemistry 1:25, Immunohistochemistry-Paraffin 1:25, Western Blot 1:1000
Protocol Western Blot protocol specific for SR-BII Antibody Procedure Guide for NB 400-102 Polyclonal anti-SR-B11Western Blot Procedure
1. Run ~50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 90 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with blocking buffer [1XTBS / 5 % NFDM / 0.1 % Tween-20] for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XTBS.
. Incubate membrane with 1:1,000 dilution of NB400-102 (anti-SR-BII), diluted in blocking buffer, overnight at 4C.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with TBST.
. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in blocking buffer, for 35 minutes at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with TBST.
. Detect cross-reacting proteins using ChemiGlow reagents from Alpha Innotech.NOTE: Jurkat whole cell extracts and HL-60 whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Western Blotting (WB) image for anti-SCARB2 antibody (Scavenger Receptor Class B, Member 2) (N-Term) (ABIN268842) Detection of SR-BII in Jurkat whole cell lysate using ABIN152881. Lane 1: 1:1000 Lane...
Product cited in: Barth, Schnober, Neumann-Haefelin et al.: "Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells." in: Journal of virology, Vol. 82, Issue 7, pp. 3466-79, 2008 (PubMed).

Zhang, Moor, Merkler et al.: "Regulation of alternative splicing of liver scavenger receptor class B gene by estrogen and the involved regulatory splicing factors." in: Endocrinology, Vol. 148, Issue 11, pp. 5295-304, 2007 (PubMed).

Huby, Doucet, Dachet et al.: "Knockdown expression and hepatic deficiency reveal an atheroprotective role for SR-BI in liver and peripheral tissues." in: The Journal of clinical investigation, Vol. 116, Issue 10, pp. 2767-76, 2006 (PubMed).

Akpovi, Yoon, Vitale et al.: "The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis." in: Journal of lipid research, Vol. 47, Issue 10, pp. 2233-47, 2006 (PubMed).

Kocher, Yesilaltay, Cirovic et al.: "Targeted disruption of the PDZK1 gene in mice causes tissue-specific depletion of the high density lipoprotein receptor scavenger receptor class B type I and altered lipoprotein metabolism." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52820-5, 2003 (PubMed).

Wadsack, Hirschmugl, Hammer et al.: "Scavenger receptor class B, type I on non-malignant and malignant human epithelial cells mediates cholesteryl ester-uptake from high density lipoproteins." in: The international journal of biochemistry & cell biology, Vol. 35, Issue 4, pp. 441-54, 2003 (PubMed).

Background publications Krieger: "The 'best' of cholesterols, the 'worst' of cholesterols: a tale of two receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4077-80, 1998 (PubMed).

Rigotti, Trigatti, Penman et al.: "A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 23, pp. 12610-5, 1997 (PubMed).

Catalog No. ABIN268842
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