Scavenger Receptor Class B, Member 2 (SCARB2) antibody

Antigen: Scavenger Receptor Class B, Member 2 (SCARB2)

Synonyms: AMRF, CD36L2, HLGP85, LIMPII, SR-BII, LGP85, Cd36l2, LIMP-2, MLGP85, 9330185J12Rik, LimpII, cd36l2, fi13c07, chunp6914, wu:fi13c07, SCARB2, scarb2, MGC140207

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN268842

Additional Information

Alternative name: SCARB2

Immunogen: A peptide containing residues from mouse SR-BII (2).

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and theirplasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI andSR-BII (previously known as SR-BI.2) are the alternatively spliced products of a singlegene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain isalmost completely different. SR-BII binds HDLs and mediates selective uptake of HDLcholesteryl ester, but with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressingSR-BI, with SR-BII expression found in liver, adrenal glands, and testes.Although the role of SR-BII is not completely clear, research suggests that it may be afunctional HDL receptor. In addition, SR-BII mRNA results from the alternative splicingof SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer oflipid between HDL and cells. The relative expression and functional activities of thesetwo isoforms create a potential means of regulating selective lipid transfer between HDLand cells. (1-6). Alternate Names: anti-SR-B2 antibody, anti-SR-BII antibody, anti-Scavenger Receptor-BII antibody,anti-Scavenger Receptor-B2 antibody. Related Diseases: Cancer

Specificity: 400-102 is specific for the SR-BII. Species Reactivity: Mouse, rat, bovine, human, mink and hamster SR-BII. Localization: Lysosomal membrane.

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 1/500 - 1/1000,Western Blot 1/1000,Immunohistochemistry-Paraffin 1/25

Buffer: serum. Preservative: 0.1% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Publications

Rigotti, Trigatti, Penman et al.: "A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 23, pp. 12610-5, 1997 (PubMed).

Krieger: "The "best" of cholesterols, the "worst" of cholesterols: a tale of two receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4077-80, 1998 (PubMed).

Wadsack, Hirschmugl, Hammer et al.: "Scavenger receptor class B, type I on non-malignant and malignant human epithelial cells mediates cholesteryl ester-uptake from high density lipoproteins." in: The international journal of biochemistry & cell biology, Vol. 35, Issue 4, pp. 441-54, 2003 (PubMed).

Kocher, Yesilaltay, Cirovic et al.: "Targeted disruption of the PDZK1 gene in mice causes tissue-specific depletion of the high density lipoprotein receptor scavenger receptor class B type I and altered lipoprotein metabolism." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52820-5, 2003 (PubMed).

Akpovi, Yoon, Vitale et al.: "The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis." in: Journal of lipid research, Vol. 47, Issue 10, pp. 2233-47, 2006 (PubMed).

Huby, Doucet, Dachet et al.: "Knockdown expression and hepatic deficiency reveal an atheroprotective role for SR-BI in liver and peripheral tissues." in: The Journal of clinical investigation, Vol. 116, Issue 10, pp. 2767-76, 2006 (PubMed).

Barth, Schnober, Neumann-Haefelin et al.: "Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells." in: Journal of virology, Vol. 82, Issue 7, pp. 3466-79, 2008 (PubMed).