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SCARB2 antibody (Scavenger Receptor Class B, Member 2) (N-Term)

Details for Product anti-SCARB2 Antibody No. ABIN268842, Supplier: Log in to see
Antigen
  • 9330185J12Rik
  • AMRF
  • cd36l2
  • Cd36l2
  • CD36L2
  • chunp6914
  • EPM4
  • fi13c07
  • HLGP85
  • LGP85
  • LIMP-2
  • LimpII
  • LIMPII
  • MLGP85
  • SCARB2
  • scarb2
  • SR-BII
  • wu:fi13c07
Epitope
N-Term, AA 400-478
17
13
6
3
3
2
2
1
1
1
1
1
Reactivity
Chinese Hamster, Cow (Bovine), Hamster, Human, Mouse (Murine), Mustelid, Rat (Rattus)
55
31
12
4
3
2
1
1
1
Host
Rabbit
42
17
6
1
Clonality
Polyclonal
Conjugate
This SCARB2 antibody is un-conjugated
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
53
38
20
14
4
4
4
4
3
1
Supplier
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Immunogen A peptide containing residues from mouse SR-BII (between residues 400-478) plus an N-terminal cysteine was coupled to KLH. [UniProt# O35114]
Purification Unpurified
Alternative Name SCARB2 (SCARB2 Antibody Abstract)
Background Gene Symbol: SCARB2
Molecular Weight Theoretical MW: 82 kDa
UniProt O35114
Application Notes Western Blot 1:1000, Flow Cytometry, Immunohistochemistry 1:25, Immunocytochemistry/Immunofluorescence 1:25-1:100, Immunohistochemistry-Paraffin 1:25
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for SR-BII Antibody Procedure Guide for NB 400-102 Polyclonal anti-SR-B11Western Blot Procedure
1. Run ~50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 90 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with blocking buffer [1XTBS / 5 % NFDM / 0.1 % Tween-20] for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XTBS.
. Incubate membrane with 1:1,000 dilution of NB400-102 (anti-SR-BII), diluted in blocking buffer, overnight at 4C.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with TBST.
. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in blocking buffer, for 35 minutes at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with TBST.
. Detect cross-reacting proteins using ChemiGlow reagents from Alpha Innotech.NOTE: Jurkat whole cell extracts and HL-60 whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-SCARB2 antibody (Scavenger Receptor Class B, Member 2) (N-Term) (ABIN268842) Detection of SR-BII in Jurkat whole cell lysate using ABIN152881. Lane 1: 1:1000 Lane...
Product cited in: Barth, Schnober, Neumann-Haefelin, Thumann, Zeisel, Diepolder, Hu, Liang, Blum, Thimme, Lambotin, Baumert: "Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells." in: Journal of virology, Vol. 82, Issue 7, pp. 3466-79, 2008

Zhang, Moor, Merkler, Liu, McLean: "Regulation of alternative splicing of liver scavenger receptor class B gene by estrogen and the involved regulatory splicing factors." in: Endocrinology, Vol. 148, Issue 11, pp. 5295-304, 2007

Huby, Doucet, Dachet, Ouzilleau, Ueda, Afzal, Rubin, Chapman, Lesnik: "Knockdown expression and hepatic deficiency reveal an atheroprotective role for SR-BI in liver and peripheral tissues." in: The Journal of clinical investigation, Vol. 116, Issue 10, pp. 2767-76, 2006

Akpovi, Yoon, Vitale, Pelletier: "The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis." in: Journal of lipid research, Vol. 47, Issue 10, pp. 2233-47, 2006

Wadsack, Hirschmugl, Hammer, Levak-Frank, Kozarsky, Sattler, Malle: "Scavenger receptor class B, type I on non-malignant and malignant human epithelial cells mediates cholesteryl ester-uptake from high density lipoproteins." in: The international journal of biochemistry & cell biology, Vol. 35, Issue 4, pp. 441-54, 2003

Kocher, Yesilaltay, Cirovic, Pal, Rigotti, Krieger: "Targeted disruption of the PDZK1 gene in mice causes tissue-specific depletion of the high density lipoprotein receptor scavenger receptor class B type I and altered lipoprotein metabolism." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52820-5, 2003

Background publications Krieger: "The 'best' of cholesterols, the 'worst' of cholesterols: a tale of two receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4077-80, 1998

Rigotti, Trigatti, Penman, Rayburn, Herz, Krieger: "A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 23, pp. 12610-5, 1997