ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (HRP)

Details for Product No. ABIN268848
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Antigen
Synonyms ABC-1, ABCA1, ABC1, CERP, HDLDT1, TGD, Abc1
Epitope
AA 1100-1300
(12), (8), (7), (7), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(66), (64), (41), (29), (12), (3), (2)
Host
Rabbit
(57), (16), (15), (2)
Clonality
Polyclonal
Conjugate
HRP
(5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(47), (36), (24), (22), (17), (13), (12), (10), (4), (4), (1)
Pubmed 13 references available
Quantity 0.1 mL
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Catalog No. ABIN268848
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Immunogen Partial peptide sequence (within residues 1100-1300) of human ABCA1. Actual immunogen sequence is proprietary information.
Cross-Reactivity (Details) Canine reactivity reported in scientific literature (PMID: 24612239)
Purification affinity purified
Alternative Name ABCA1
Background Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reportedin Tangier disease (TD). TD is an autosomal recessive disorder characterized by anabsence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial systemand deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associatedexport of cholesterol and phospholipids from the cell. It is expressed on the plasmamembrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of thecholesterol flux between HDL and macrophages is competitive between ABCA1 andSR-BI. Alternate Names: anti-ABC 1 antibody, anti-ABC Transporter 1 antibody, anti-ABC1 antibody,anti-ATP-binding Cassette 1 antibody, anti-ATP-binding Cassette Transporter 1antibody, anti-CERP antibody, anti-Cholesterol Efflux Regulatory Protein antibody,anti-ABC-1 antibody. Related Diseases: Tangier Disease, Atherosclerosis, Coronary Artery Disease
Gene Symbol: ABCA1
Gene ID 19, 11303, 313210
UniProt O95477
Application Notes This ABCA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence (PMID: 21501868), Immunoprecipitation (PMID: 21846716, 21106522), Western blot and Immunohistochemistry on frozen and paraffin embedded sections. In Western blot a band representing ABCA1 is observed at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. NOTE: It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Chromatin Immunoprecipitation and ELISA were reported in scientific literature.
Recommended dilutions: Immunoprecipitation 1:10-1:500, Western Blot 1:500
Comment

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Storage Comment 4 °C in the dark
General Burgess, Kiss, Zheng et al.: "Trypsin-sensitive and lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I and participate in ABCA1-dependent cholesterol efflux." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 31318-26, 2002 (PubMed).

Murthy, Born, Mathur et al.: "LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells." in: Journal of lipid research, Vol. 43, Issue 7, pp. 1054-64, 2002 (PubMed).

Feng, Tabas: "ABCA1-mediated cholesterol efflux is defective in free cholesterol-loaded macrophages. Mechanism involves enhanced ABCA1 degradation in a process requiring full NPC1 activity." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 43271-80, 2002 (PubMed).

Fukumoto, Deng, Irizarry et al.: "Induction of the cholesterol transporter ABCA1 in central nervous system cells by liver X receptor agonists increases secreted Abeta levels." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48508-13, 2002 (PubMed).

Wang, Chen, Linsel-Nitschke et al.: "A PEST sequence in ABCA1 regulates degradation by calpain protease and stabilization of ABCA1 by apoA-I." in: The Journal of clinical investigation, Vol. 111, Issue 1, pp. 99-107, 2003 (PubMed).

Escher, Krozowski, Croft et al.: "Expression of sterol 27-hydroxylase (CYP27A1) enhances cholesterol efflux." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11015-9, 2003 (PubMed).

Koldamova, Lefterov, Ikonomovic et al.: "22R-hydroxycholesterol and 9-cis-retinoic acid induce ATP-binding cassette transporter A1 expression and cholesterol efflux in brain cells and decrease amyloid beta secretion." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 13244-56, 2003 (PubMed).

Basso, Freeman, Knapper et al.: "Role of the hepatic ABCA1 transporter in modulating intrahepatic cholesterol and plasma HDL cholesterol concentrations." in: Journal of lipid research, Vol. 44, Issue 2, pp. 296-302, 2003 (PubMed).

Terasaka, Hiroshima, Koieyama et al.: "T-0901317, a synthetic liver X receptor ligand, inhibits development of atherosclerosis in LDL receptor-deficient mice." in: FEBS letters, Vol. 536, Issue 1-3, pp. 6-11, 2003 (PubMed).

Sun, Yao, Kim et al.: "Expression of liver X receptor target genes decreases cellular amyloid beta peptide secretion." in: The Journal of biological chemistry, Vol. 278, Issue 30, pp. 27688-94, 2003 (PubMed).

Le Lay, Robichon, Le Liepvre et al.: "Regulation of ABCA1 expression and cholesterol efflux during adipose differentiation of 3T3-L1 cells." in: Journal of lipid research, Vol. 44, Issue 8, pp. 1499-507, 2003 (PubMed).

Khovidhunkit, Moser, Shigenaga et al.: "Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR." in: Journal of lipid research, Vol. 44, Issue 9, pp. 1728-36, 2003 (PubMed).

Witting, Maiorano, Davidson: "Ceramide enhances cholesterol efflux to apolipoprotein A-I by increasing the cell surface presence of ATP-binding cassette transporter A1." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 40121-7, 2003 (PubMed).

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