ATP-Binding Cassette, Sub-Family G (WHITE), Member 8 (ABCG8) (N-Term) antibody

Details for Product No. ABIN268853
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Antigen
Synonyms DDBDRAFT_0167690, DDBDRAFT_0191232, DDB_0167690, DDB_0191232, zgc:172358, GBD4, STSL, 1300003C16Rik, AI114946, sterolin-2
Epitope
N-Term
(8)
Reactivity
Human
(51), (37), (31), (25), (25), (25)
Host
Rabbit
(53), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application
Western Blotting (WB)
(35), (20), (11), (6), (5), (4), (1)
Pubmed 6 references available
Quantity 0.025 mL
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Catalog No. ABIN268853
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Immunogen A synthetic peptide from the N-terminal region of human ABCG8 protein.
No Cross-Reactivity Mouse (Murine)
Purification affinity purified
Alternative Name ABCG8
Background ATP-binding cassette (ABC) transporter genes are involved in the regulation of theamount of dietary cholesterol retained in the body. ABCG8, expressed at high levels inthe liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption andpromote biliary excretion of sterols. The mutated form of this transporter can lead tosterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessivedisorder, characterized by hyperabsorption of sterols and the inability to excrete sterolsinto bile. Alternate Names: anti-ATP binding cassette sub family G (WHITE) member 8 (sterolin 2) antibody,anti-ATP binding cassette sub family G member 8 antibody, anti-MGC142217 antibody,anti-Sterolin 2 antibody, anti-STSL antibody, anti-ATP binding cassette G8 antibody. Related Diseases: Atherosclerosis, Coronary Artery Disease
Gene Symbol: ABCG8
Gene ID 64241
UniProt Q9H221
Research Area Cancer, Atherosclerosis, Metabolism
Application Notes This ABCG8 antibody is useful for Western blot. The antibody does not appear to be applicable in immunohistochemistry.
Recommended dilutions: Immunohistochemistry (Negative), Western Blot 1:500
Protocol Protocol specific for ABCG8 Antibody Western Blot Protocol
1. Load ~50 micrograms of lysate (ie: liver membrane) onto an 8 % SDS-PAGE, reducing.
. Block the protein transferred membrane for 30 minutes in blocking buffer [5 % NFDM, 10 mM Tris (pH 7.
6) saline, 0.2 % Tween-20] at room temperature.
. Incubate the membrane in diluted primary anti-ABCG8 [cat# NB 400-110], 1:500 for 1 hour at room temperature.
. Wash the membrane 3x 5 minutes in blocking buffer without milk.
. Incubate the membrane in diluted secondary anti-Rabbit IgG conjugated to HRP.
. Wash the membrane 3x 5 minutes in blocking buffer without milk.
. Wash the membrane for 5 minutes in TBS.
. Develop using West-Pico ECL (Pierce) reagent.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
General Garcia, Wilund, Arca et al.: "Autosomal recessive hypercholesterolemia caused by mutations in a putative LDL receptor adaptor protein." in: Science (New York, N.Y.), Vol. 292, Issue 5520, pp. 1394-8, 2001 (PubMed).

Lu, Lee, Hazard et al.: "Two genes that map to the STSL locus cause sitosterolemia: genomic structure and spectrum of mutations involving sterolin-1 and sterolin-2, encoded by ABCG5 and ABCG8, respectively." in: American journal of human genetics, Vol. 69, Issue 2, pp. 278-90, 2001 (PubMed).

Schmitz, Langmann, Heimerl: "Role of ABCG1 and other ABCG family members in lipid metabolism." in: Journal of lipid research, Vol. 42, Issue 10, pp. 1513-20, 2001 (PubMed).

Remaley, Bark, Walts et al.: "Comparative genome analysis of potential regulatory elements in the ABCG5-ABCG8 gene cluster." in: Biochemical and biophysical research communications, Vol. 295, Issue 2, pp. 276-82, 2002 (PubMed).

Kobayashi, Takanezawa, Hirata et al.: "Efflux of sphingomyelin, cholesterol, and phosphatidylcholine by ABCG1." in: Journal of lipid research, Vol. 47, Issue 8, pp. 1791-802, 2006 (PubMed).

Mathur, Watt, Field: "Regulation of intestinal NPC1L1 expression by dietary fish oil and docosahexaenoic acid." in: Journal of lipid research, Vol. 48, Issue 2, pp. 395-404, 2007 (PubMed).

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