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ABCG8 antibody (ATP-Binding Cassette, Sub-Family G (WHITE), Member 8) (N-Term)

Details for Product anti-ABCG8 Antibody No. ABIN268853, Supplier: Log in to see
Antigen
  • 1300003C16Rik
  • AI114946
  • DDBDRAFT_0167690
  • DDBDRAFT_0191232
  • DDB_0167690
  • DDB_0191232
  • GBD4
  • sterolin-2
  • STSL
  • zgc:172358
Epitope
N-Term
26
2
1
1
Reactivity
Human
62
38
11
Host
Rabbit
52
13
Clonality
Polyclonal
Conjugate
This ABCG8 antibody is un-conjugated
5
5
4
4
3
3
3
3
3
3
3
Application
Western Blotting (WB)
65
21
18
18
16
13
6
1
Supplier
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Supplier Product No.
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Immunogen A synthetic peptide from the N-terminal region of human ABCG8 protein. [UniProt# Q9H221]
No Cross-Reactivity Mouse (Murine)
Purification Immunogen affinity purified
Alternative Name ABCG8 (ABCG8 Antibody Abstract)
Background Gene Symbol: ABCG8
UniProt Q9H221
Research Area Cancer, Atherosclerosis, Metabolism
Application Notes Western Blot 1:500

Not for use in: Immunohistochemistry
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for ABCG8 Antibody Western Blot Protocol
1. Load ~50 micrograms of lysate (ie: liver membrane) onto an 8 % SDS-PAGE, reducing.
. Block the protein transferred membrane for 30 minutes in blocking buffer [5 % NFDM, 10 mM Tris (pH 7.
6) saline, 0.2 % Tween-20] at room temperature.
. Incubate the membrane in diluted primary anti-ABCG8 [cat# NB 400-110], 1:500 for 1 hour at room temperature.
. Wash the membrane 3x 5 minutes in blocking buffer without milk.
. Incubate the membrane in diluted secondary anti-Rabbit IgG conjugated to HRP.
. Wash the membrane 3x 5 minutes in blocking buffer without milk.
. Wash the membrane for 5 minutes in TBS.
. Develop using West-Pico ECL (Pierce) reagent.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-ABCG8 antibody (ATP-Binding Cassette, Sub-Family G (WHITE), Member 8) (N-Term) (ABIN268853) Western blot analysis of ABCG8, using ABIN152887. Samples: mouse liver microsomes fro...
Product cited in: Maqdasy, El Hajjaji, Baptissart, Viennois, Oumeddour, Brugnon, Trousson, Tauveron, Volle, Lobaccaro, Baron: "Identification of the Functions of Liver X Receptor-β in Sertoli Cells Using a Targeted Expression-Rescue Model." in: Endocrinology, Vol. 156, Issue 12, pp. 4545-57, 2015 (PubMed). (Sample species: Mouse (Murine)).

Suzuki, Shuto, Sato, Kaneko, Takada, Suico, Cyr, Suzuki, Kai: "Inhibition of post-translational N-glycosylation by HRD1 that controls the fate of ABCG5/8 transporter." in: Scientific reports, Vol. 4, pp. 4258, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Renner, Lütjohann, Richter, Strohmeyer, Schimmel, Müller, Stange, Harsch: "Role of the ABCG8 19H risk allele in cholesterol absorption and gallstone disease." in: BMC gastroenterology, Vol. 13, pp. 30, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Mathur, Watt, Field: "Regulation of intestinal NPC1L1 expression by dietary fish oil and docosahexaenoic acid." in: Journal of lipid research, Vol. 48, Issue 2, pp. 395-404, 2007 (PubMed).

Kobayashi, Takanezawa, Hirata, Shimizu, Misasa, Kioka, Arai, Ueda, Matsuo: "Efflux of sphingomyelin, cholesterol, and phosphatidylcholine by ABCG1." in: Journal of lipid research, Vol. 47, Issue 8, pp. 1791-802, 2006 (PubMed). (Sample species: Human). Further details: Western Blotting

Background publications Lu, Lee, Hazard, Brooks-Wilson, Hidaka, Kojima, Ose, Stalenhoef, Mietinnen, Bjorkhem, Bruckert, Pandya, Brewer, Salen, Dean, Srivastava, Patel: "Two genes that map to the STSL locus cause sitosterolemia: genomic structure and spectrum of mutations involving sterolin-1 and sterolin-2, encoded by ABCG5 and ABCG8, respectively." in: American journal of human genetics, Vol. 69, Issue 2, pp. 278-90, 2001 (PubMed).

Garcia, Wilund, Arca, Zuliani, Fellin, Maioli, Calandra, Bertolini, Cossu, Grishin, Barnes, Cohen, Hobbs: "Autosomal recessive hypercholesterolemia caused by mutations in a putative LDL receptor adaptor protein." in: Science (New York, N.Y.), Vol. 292, Issue 5520, pp. 1394-8, 2001 (PubMed).

Schmitz, Langmann, Heimerl: "Role of ABCG1 and other ABCG family members in lipid metabolism." in: Journal of lipid research, Vol. 42, Issue 10, pp. 1513-20, 2001 (PubMed).