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SCARB1 antibody (Scavenger Receptor Class B, Member 1) (AA 230-380)

Details for Product anti-SCARB1 Antibody No. ABIN268870, Supplier: Log in to see
Antigen
  • AI120173
  • cb1015
  • CD36
  • CD36L1
  • Cd36l1
  • CLA-1
  • Cla-1
  • CLA1
  • Cla1
  • D5Ertd460e
  • Hdlq1
  • HDLQTL6
  • Hlb398
  • mSR-BI
  • SCARB1
  • Scarb1
  • SR-B1
  • SR-BI
  • Srb1
  • SRB1
  • SRBI
Epitope
AA 230-380
43
25
23
15
13
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
91
64
59
31
23
23
19
12
12
11
11
8
1
Host
Rabbit
76
18
15
Clonality
Polyclonal
Conjugate
This SCARB1 antibody is un-conjugated
6
5
4
4
4
4
3
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
91
46
45
35
32
28
28
20
10
7
4
1
1
1
Supplier
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Immunogen A peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII that was expressed as two tandem copies in bacteria using the pET system.
Cross-Reactivity (Details) Previous lots have been shown to react with rat,and human as well.
Purification Unpurified
Alternative Name SCARB1 (SCARB1 Antibody Abstract)
Background Gene Symbol: SCARB1
Molecular Weight Theoretical MW: 82 kDa
UniProt Q61009, O35114
Application Notes Western Blot 1:1000, Flow Cytometry 1:400, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunoprecipitation
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol for SR-BI/SR-BII Antibody Western Blot Procedure
1. Run 80 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 60 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/5 % non-fat milk/0.1 % Tween-20 for 1 hour at room temperature (23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with 1:1,000 dilution of NB400-134 (anti-SR-BI/BII), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with dilution of goat anti-rabbit IgG-HRP (secondary), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Chemiluminescence reagents. ICC/IF Protocol for SR-BI/SR-BII Antibody Immunocytochemistry ProtocolCulture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 5-10 minutes.
. Remove the formalin and add 0.5 % Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1 % Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
. To block nonspecific antibody binding incubate in 10 % normal goat serum for 1 hour at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 µg/ml, or coverslips can be directly mounted in media containing DAPI.
. Cells can now be viewed with a fluorescence microscope.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-SCARB1 antibody (Scavenger Receptor Class B, Member 1) (AA 230-380) (ABIN268870) anti-Scavenger Receptor Class B, Member 1 (SCARB1) (AA 230-380) antibody
Product cited in: Harder, Meng, Rippstein, McBride, McPherson: "SR-BI undergoes cholesterol-stimulated transcytosis to the bile canaliculus in polarized WIF-B cells." in: The Journal of biological chemistry, Vol. 282, Issue 2, pp. 1445-55, 2007 (PubMed).

Kocher, Yesilaltay, Cirovic, Pal, Rigotti, Krieger: "Targeted disruption of the PDZK1 gene in mice causes tissue-specific depletion of the high density lipoprotein receptor scavenger receptor class B type I and altered lipoprotein metabolism." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52820-5, 2003 (PubMed).

Silver: "A carboxyl-terminal PDZ-interacting domain of scavenger receptor B, type I is essential for cell surface expression in liver." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34042-7, 2002 (PubMed).