Androgen Receptor antibody

Catalog No. ABIN2688843

Product Details anti-Androgen Receptor Antibody

Target: Androgen Receptor (AR)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The androgen receptor (AR) mediating androgen effects is a member of the steroid and thyroid hormone receptor gene superfamily encoding ligand-dependent nuclear transcription factors. They can be divided into three main domains, an N-terminal domain which modulates transcription efficiency, a central DNA domain which binds to a target gene hormone response element, and a C-terminal hormone binding domain. Androgens are necessary for normal male development and function. They exert their effects on target tissue through binding to the AR, followed by association of the AR complex with specific binding sites on DNA. This AR complex may induce or repress gene transcription. Mutations in the AR gene are associated with androgen insensitivity syndrome, a disorder that causes XY genotypic males to develop as phenotypic females because of their inability to respond to androgens. AR mutations have also been identified in human prostate cancer specimens and the LNCaP human prostate cancer cell line. Monoclonal and polyclonal antibodies to the AR have identified AR-positive cells in a variety of tissues including male and female sexual organs, kidney, liver, adrenal cortex, pituitary gland, skeletal, cardiac, and smooth muscle cells. The androgen receptor migrates at a molecular weight of ~100 kD in SDS-PAGE. G122-25 recognizes an epitope localized within the DNA-binding domain (between amino acids 486 and 651) of the human androgen receptor. Additionally, G122-25 has been shown to recognize androgen receptors in frozen rat prostate tissue sections. G122-25 does not cross-react with estrogen or progesterone receptors. Purified recombinant human androgen receptor protein was used as immunogen.

BD Pharmingen™ Purified Mouse Anti-Human Androgen Receptor - Purified - Clone G122-25 - Isotype Mouse IgG1 - Reactivity Hu, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified human androgen receptor recombinant protein

Clone: G122

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Androgen Receptor antibody (ABIN2688843)

Fuller: "The steroid receptor superfamily: mechanisms of diversity." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 5, Issue 15, pp. 3092-9, (1992) (PubMed).

Newmark, Hardy, Tonb, Carter, Epstein, Isaacs, Brown, Barrack: "Androgen receptor gene mutations in human prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6319-23, (1992) (PubMed).

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor (AR Products)

Synonyms: AIS antibody, DHTR antibody, HUMARA antibody, HYSP1 antibody, KD antibody, NR3C4 antibody, SBMA antibody, SMAX1 antibody, TFM antibody, AW320017 antibody, Tfm antibody, Andr antibody, AR antibody, androgen receptor antibody, AR antibody, Ar antibody, ar antibody

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding