KIT antibody (Biotin)

Catalog No. ABIN2688895

Product Details anti-KIT Antibody (Biotin)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This KIT antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 2B8 antibody reacts with CD117 (c-Kit), a transmembrane tyrosine-kinase receptor which is encoded by the Kit gene (formerly dominant white spotting, W). The c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor) encoded by the Kit1 gene (formerly steel, SI), is a co-mitogen for hematopoietic stem cells, myeloerythroid progenitors and a mast-cell differentiation factor. The KitW and Kit1SI mutant alleles have similar pleiotropic effects on the development of melanocytes, germ cells, and the hematopoietic system. In the adult bone marrow, CD117 is expressed on hematopoietic progenitor cells, including CD90 (Thy-1) low, TER-119-, CD45R/B220-, CD11b (Mac-1)-, Ly-6G (Gr-1)-, CD4-, CD8-, and Sca-1 (Ly-6A/E)+ multipotent hemotopoietic stem cells, progenitors committed to myeliod and/or erythroid lineages, and precursors of B and T lymphocytes. This widespread expression of CD117 in hematopoietic precursors is consistent with the participation of c-Kit and its ligand in the regulation of several hematopoietic lineages. Intrathymic expression of c-Kit and c-Kit ligand suggest that CD117 is also involved in the regulation of some events during the development of T lymphocytes. CD117 is also expressed by mast cells and by dendritic cells found in the periarteriolar lymphocytoc sheaths (T-cell areas) of splenic white pulp. The mAb 2B8 reportedly does not block the action of c-Kit. This clone 2B8 had been reported that there was cross-reactivity with rat. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD117 on mouse bone marrow cells. A single-cell suspension of BALB/c bone marrow was simultaneously stained with PE-conjugated RA3-6B2 (anti-mouse CD45R/B220, Cat. No. 553089/553090, both panels) and biotin-conjugated 2B8 (bottom) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Biotin Rat Anti-Mouse CD117 - Biotin - Clone 2B8 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Bone Marrow Mast Cells

Clone: 2B8

Isotype: IgG2b kappa

Application Details

Application Notes: Flow Cytometry System.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-KIT antibody (Biotin) (ABIN2688895)

Fadini, Sartore, Schiavon, Albiero, Baesso, Cabrelle, Agostini, Avogaro: "Diabetes impairs progenitor cell mobilisation after hindlimb ischaemia-reperfusion injury in rats." in: Diabetologia, Vol. 49, Issue 12, pp. 3075-84, (2006) (PubMed).

Austen, Boyce: "Mast cell lineage development and phenotypic regulation." in: Leukemia research, Vol. 25, Issue 7, pp. 511-8, (2001) (PubMed).

Domen, Weissman: "Hematopoietic stem cells need two signals to prevent apoptosis; BCL-2 can provide one of these, Kitl/c-Kit signaling the other." in: The Journal of experimental medicine, Vol. 192, Issue 12, pp. 1707-18, (2001) (PubMed).

Mirmonsef, Shelburne, Fitzhugh Yeatman, Chong, Ryan: "Inhibition of Kit expression by IL-4 and IL-10 in murine mast cells: role of STAT6 and phosphatidylinositol 3'-kinase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2530-9, (1999) (PubMed).

Lian, Toki, Yu, Hayashi, Yasumizu, Sugiura, Jin, Inaba, Hisha, Li, Yu, Fan, Ikehara: "Intrathymically injected hemopoietic stem cells can differentiate into all lineage cells in the thymus: differences between c-kit+ cells and c-kit < low cells." in: Stem cells (Dayton, Ohio), Vol. 15, Issue 6, pp. 430-6, (1998) (PubMed).

Kondo, Weissman, Akashi: "Identification of clonogenic common lymphoid progenitors in mouse bone marrow." in: Cell, Vol. 91, Issue 5, pp. 661-72, (1997) (PubMed).

Pulendran, Lingappa, Kennedy, Smith, Teepe, Rudensky, Maliszewski, Maraskovsky: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, (1997) (PubMed).

Rodewald, Kretzschmar, Swat, Takeda: "Intrathymically expressed c-kit ligand (stem cell factor) is a major factor driving expansion of very immature thymocytes in vivo." in: Immunity, Vol. 3, Issue 3, pp. 313-9, (1995) (PubMed).

Godfrey, Zlotnik: "Control points in early T-cell development." in: Immunology today, Vol. 14, Issue 11, pp. 547-53, (1994) (PubMed).

Valent: "The riddle of the mast cell: kit(CD117)-ligand as the missing link?" in: Immunology today, Vol. 15, Issue 3, pp. 111-4, (1994) (PubMed).

Ikuta, Weissman: "Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 4, pp. 1502-6, (1992) (PubMed).

Ogawa, Matsuzaki, Nishikawa, Hayashi, Kunisada, Sudo, Kina, Nakauchi, Nishikawa: "Expression and function of c-kit in hemopoietic progenitor cells." in: The Journal of experimental medicine, Vol. 174, Issue 1, pp. 63-71, (1991) (PubMed).

Anderson, Lyman, Baird, Wignall, Eisenman, Rauch, March, Boswell, Gimpel, Cosman: "Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms." in: Cell, Vol. 63, Issue 1, pp. 235-43, (1990) (PubMed).

Huang, Nocka, Beier, Chu, Buck, Lahm, Wellner, Leder, Besmer: "The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus." in: Cell, Vol. 63, Issue 1, pp. 225-33, (1990) (PubMed).

Zsebo, Wypych, McNiece, Lu, Smith, Karkare, Sachdev, Yuschenkoff, Birkett, Williams: "Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium." in: Cell, Vol. 63, Issue 1, pp. 195-201, (1990) (PubMed).

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: CD117 (KIT Products)

Synonyms: C-Kit antibody, CD117 antibody, PBT antibody, SCFR antibody, Bs antibody, Fdc antibody, Gsfsco1 antibody, Gsfsco5 antibody, Gsfsow3 antibody, SCO1 antibody, SCO5 antibody, SOW3 antibody, Ssm antibody, Tr-kit antibody, W antibody, c-KIT antibody, c-kit antibody, MGF antibody, KIT antibody, C-KIT antibody, kit antibody, receptor antibody, sparse antibody, Xkl-1 antibody, kit-a antibody, kit-b antibody, kl1-A antibody, krk1 antibody, pbt antibody, scfr antibody, KIT proto-oncogene receptor tyrosine kinase antibody, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a antibody, KIT proto-oncogene receptor tyrosine kinase S homeolog antibody, KIT antibody, Kit antibody, kita antibody, kit antibody, kit.S antibody

Background: Synonyms: c-Kit

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity