IL-6 Receptor antibody (Biotin)

Catalog No. ABIN2688946

Product Details anti-IL-6 Receptor Antibody (Biotin)

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-6 Receptor antibody is conjugated to Biotin

Application: ELISA

Brand: BD Pharmingen™

Characteristics: The M182 antibody reacts with the a subunit of the human IL-6 Receptor (IL-6Rα, aka, CD126). The human IL-6Rα is an 80 kDa type I transmembrane glycoprotein, also known as B cell stimulatory factor-2 (BSF-2) receptor and IL-6 receptor. The IL-6Rα subunit associates with the 130-160 kDa gp130 subunit (IL-6 receptor ß chain, CD130), that is shared with the receptors for Leukemia Inhibitory Factor (LIF), Ciliary Neurotropic Factor (CNTF), Oncostatin M (OSM), IL-11, Cardiotropin 1 (CT-1) and possibly Neurotrophin-1/B Cell-Stimulating Factor 3 (NNT-1/BSF-3). The IL-6Rα chain binds IL-6 with low affinity, however the association with CD130 stabilizes the IL-6/IL-6Rα complex resulting in the formation of a high affinity complex. The IL-6R ß chain mediates signal transduction. IL-6Rα's are expressed at high levels by activated and EBV-transformed B cells, plasma cells and myeloma cells and at lower levels by most leucocytes, epithelial cells, fibroblasts, hepatocytes and neural cells. IL-6Rα exists in soluble form in human serum. The serum levels of soluble IL-6Raappear to elevate in pathological situations such as multiple myeloma, Grave's disease, juvenile chronic arthritis and HIV.10-13 The immunogen used to generate the M182 hybridoma was a mixture of U266, XG-1 (human myeloma cell line expressing membrane IL-6R) and BWD41 cells (murine thymoma cell line transfected with cDNA encoding the extracellular part of IL-6Rα).

BD Pharmingen™ Biotin Mouse Anti-Human CD126 - Biotin - Clone M182 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mixture of U266, XG-1and BWD41 cells

Clone: M182

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-6 Receptor antibody (Biotin) (ABIN2688946)

Senaldi, Varnum, Sarmiento, Starnes, Lile, Scully, Guo, Elliott, McNinch, Shaklee, Freeman, Manu, Simonet, Boone, Chang: "Novel neurotrophin-1/B cell-stimulating factor-3: a cytokine of the IL-6 family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11458-63, (1999) (PubMed).

Heinrich, Behrmann, Müller-Newen, Schaper, Graeve: "Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway." in: The Biochemical journal, Vol. 334 ( Pt 2), pp. 297-314, (1998) (PubMed).

Hirano, Nakajima, Hibi: "Signaling mechanisms through gp130: a model of the cytokine system." in: Cytokine & growth factor reviews, Vol. 8, Issue 4, pp. 241-52, (1998) (PubMed).

Keul, Heinrich, Müller-newen, Muller, Woo: "A possible role for soluble IL-6 receptor in the pathogenesis of systemic onset juvenile chronic arthritis." in: Cytokine, Vol. 10, Issue 9, pp. 729-34, (1998) (PubMed).

Müller-Newen, Köhne, Keul, Hemmann, Müller-Esterl, Wijdenes, Brakenhoff, Hart, Heinrich: "Purification and characterization of the soluble interleukin-6 receptor from human plasma and identification of an isoform generated through alternative splicing." in: European journal of biochemistry / FEBS, Vol. 236, Issue 3, pp. 837-42, (1996) (PubMed).

Salvi, Girasole, Pedrazzoni, Passeri, Giuliani, Minelli, Braverman, Roti: "Increased serum concentrations of interleukin-6 (IL-6) and soluble IL-6 receptor in patients with Graves' disease." in: The Journal of clinical endocrinology and metabolism, Vol. 81, Issue 8, pp. 2976-9, (1996) (PubMed).

Browning, Dougas, Ngam-ek, Bourdon, Ehrenfels, Miatkowski, Zafari, Yampaglia, Lawton, Meier: "Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 33-46, (1995) (PubMed).

Liautard, Gaillard, Mani, Montero-Julian, Duperray, Lu, Jourdan, Klein, Brailly, Brochier: "Epitope analysis of human IL-6 receptor gp80 molecule with monoclonal antibodies." in: European cytokine network, Vol. 5, Issue 3, pp. 293-300, (1994) (PubMed).

Gaillard, Bataille, Brailly, Zuber, Yasukawa, Attal, Maruo, Taga, Kishimoto, Klein: "Increased and highly stable levels of functional soluble interleukin-6 receptor in sera of patients with monoclonal gammopathy." in: European journal of immunology, Vol. 23, Issue 4, pp. 820-4, (1993) (PubMed).

Honda, Yamamoto, Cheng, Yasukawa, Suzuki, Saito, Osugi, Tokunaga, Kishimoto: "Human soluble IL-6 receptor: its detection and enhanced release by HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 7, pp. 2175-80, (1992) (PubMed).

Hibi, Murakami, Saito, Hirano, Taga, Kishimoto: "Molecular cloning and expression of an IL-6 signal transducer, gp130." in: Cell, Vol. 63, Issue 6, pp. 1149-57, (1991) (PubMed).

Van Snick: "Interleukin-6: an overview." in: Annual review of immunology, Vol. 8, pp. 253-78, (1990) (PubMed).

Taga, Hibi, Hirata, Yamasaki, Yasukawa, Matsuda, Hirano, Kishimoto: "Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130." in: Cell, Vol. 58, Issue 3, pp. 573-81, (1989) (PubMed).

Yamasaki, Taga, Hirata, Yawata, Kawanishi, Seed, Taniguchi, Hirano, Kishimoto: "Cloning and expression of the human interleukin-6 (BSF-2/IFN beta 2) receptor." in: Science (New York, N.Y.), Vol. 241, Issue 4867, pp. 825-8, (1988) (PubMed).

Taga, Kawanishi, Hardy, Hirano, Kishimoto: "Receptors for B cell stimulatory factor 2. Quantitation, specificity, distribution, and regulation of their expression." in: The Journal of experimental medicine, Vol. 166, Issue 4, pp. 967-81, (1987) (PubMed).

Target Details for IL-6 Receptor

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Alternative Name: CD126 (IL6R Products)

Synonyms: IL6R antibody, il-6ra antibody, CD126 antibody, IL-6R-1 antibody, IL-6RA antibody, IL6Q antibody, IL6RA antibody, IL6RQ antibody, gp80 antibody, IL6R1 antibody, Il6ra antibody, IL-6Ralpha antibody, IL-6R antibody, Il6r antibody, interleukin 6 receptor antibody, interleukin 6 receptor, alpha antibody, IL6R antibody, il6r antibody, Il6r antibody, Il6ra antibody

Background: Synonyms: IL-6 Receptor α Chain

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints