CD137 antibody (Biotin)

Catalog No. ABIN2688964

Product Details anti-CD137 Antibody (Biotin)

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD137 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 1AH2 clone (subclone of 53A2) has been reported to react with CD137, a member of the TNFR/NGFR superfamily. The expression of CD137 has been reported to be detectable from day 3 and peaks around day 6 after activation with ConA, PMA plus ionomycin, or immobilized anti-CD3e (Cat. No. 553058). Monomers, dimers, or tetramers of the 4-1BB antigen are expressed, upon activation, on the surface of splenic T lymphocytes, thymocytes, intestinal intraepithelial T lymphocytes (IEL), and some T cell lines and clones. While stimulating T cells by IL-2, IL-4, or anti-CD28 alone does not result in the expression of CD137, addition of IL-2, IL-4, anti-CD28, or syngeneic accessory cells to splenic T cells stimulated via TCR/CD3 can result in a high level of CD137 expression. CD137 has also been reported to be observed on IL-2 activated NK cells, but not on freshly isolated NK cells. It has been demonstrated that 4-1BB physically associates with p56 [lck]through a Cys-Arg-Cys-Pro binding site in its cytoplasmic domain, the same motif in the cytoplasmic tail of the CD4 and CD8a molecules is responsible for association with p56 [lck]. A signaling function for the CD137 Molecule in mouse T cells is indicated by reports in which cross-linking of CD137 with 1AH2 mAb resulted in enhanced proliferation of CD3e-activated splenic T cells and IEL and in enhanced cytolytic activity of IEL in response to immobilized anti-CD3e. In addition to extracellular matrix proteins which bind to CD137, a 97- kDa dimer of the TNF/NGF superfamily has been reported to be a ligand for 4-1BB (4-1BBL). This molecule has been detected on Con A-activated T cells, LPS-activated macrophages, and anti-μ-activated splenic B cells. Interaction between T and B cells through 4-1BB/4-1BBL is reported to play a role in antigen presentation, further supporting a costimulatory role for CD137 in the immune response of T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD137 - Biotin - Clone 1AH2 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant mouse 4-1BB

Clone: 1AH2

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD137 antibody (Biotin) (ABIN2688964)

Takeda, Oshima, Hayakawa, Akiba, Atsuta, Kobata, Kobayashi, Ito, Yagita, Okumura: "CD27-mediated activation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1741-5, (2000) (PubMed).

Saoulli, Lee, Cannons, Yeh, Santana, Goldstein, Bangia, DeBenedette, Mak, Choi, Watts: "CD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand." in: The Journal of experimental medicine, Vol. 187, Issue 11, pp. 1849-62, (1998) (PubMed).

DeBenedette, Shahinian, Mak, Watts: "Costimulation of CD28- T lymphocytes by 4-1BB ligand." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 551-9, (1997) (PubMed).

Hurtado, Kim, Kwon: "Signals through 4-1BB are costimulatory to previously activated splenic T cells and inhibit activation-induced cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 6, pp. 2600-9, (1997) (PubMed).

DeBenedette, Chu, Pollok, Hurtado, Wade, Kwon, Watts: "Role of 4-1BB ligand in costimulation of T lymphocyte growth and its upregulation on M12 B lymphomas by cAMP." in: The Journal of experimental medicine, Vol. 181, Issue 3, pp. 985-92, (1995) (PubMed).

Hurtado, Kim, Pollok, Lee, Kwon: "Potential role of 4-1BB in T cell activation. Comparison with the costimulatory molecule CD28." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 7, pp. 3360-7, (1995) (PubMed).

Pollok, Kim, Kwon: "Regulation of 4-1BB expression by cell-cell interactions and the cytokines, interleukin-2 and interleukin-4." in: European journal of immunology, Vol. 25, Issue 2, pp. 488-94, (1995) (PubMed).

Zhou, Pollok, Kim, Kim, Kwon: "Functional analysis of T-cell antigen 4-1BB in activated intestinal intra-epithelial T lymphocytes." in: Immunology letters, Vol. 41, Issue 2-3, pp. 177-84, (1995) (PubMed).

Kim, Pollok, Zhou, Shaw, Bohlen, Fraser, Kwon: "Novel T cell antigen 4-1BB associates with the protein tyrosine kinase p56lck1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1255-62, (1993) (PubMed).

Pollok, Kim, Zhou, Hurtado, Kim, Pickard, Kwon: "Inducible T cell antigen 4-1BB. Analysis of expression and function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 3, pp. 771-81, (1993) (PubMed).

Chalupny, Peach, Hollenbaugh, Ledbetter, Farr, Aruffo: "T-cell activation molecule 4-1BB binds to extracellular matrix proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 21, pp. 10360-4, (1992) (PubMed).

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: CD137 (TNFRSF9 Products)

Synonyms: TNFRSF9 antibody, zgc:136557 antibody, 4-1BB antibody, CD137 antibody, CDw137 antibody, ILA antibody, A930040I11Rik antibody, AA408498 antibody, AI325004 antibody, Cd137 antibody, Ly63 antibody, tumor necrosis factor receptor superfamily member 9 antibody, TNF receptor superfamily member 9 antibody, tumor necrosis factor receptor superfamily, member 9a antibody, tumor necrosis factor receptor superfamily, member 9 antibody, TNFRSF9 antibody, tnfrsf9a antibody, Tnfrsf9 antibody

Background: Synonyms: 4-1BB, Ly-63

Pathways: Cancer Immune Checkpoints