CTLA4 antibody

Catalog No. ABIN2688989

Product Details anti-CTLA4 Antibody

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: The UC10-4F10-11 antibody reacts with CD152 (CTLA-4), which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor. CTLA-4 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. Whereas CD28 delivers a costimulatory signal required for T-cell activation, CTLA-4 is a negative regulator of cell-mediated immune responses. CD152 may play roles in induction and/or maintenance of immunological tolerance, regulation of protective immunity, and autoimmune responses, and regulation of some aspects of thymocyte maturation. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes. Expression of CD152 on activated T lymphocytes. C57BL/6 splenocytes were stained with either purified mAb UC10-4F10-11 (open histogram) or unstained (filled histogram), in the presence of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142), followed by biotinylated anti-hamster IgG cocktail (Cat. No. 554010) then Streptavidin-PE (Cat. No. 554061). The total viable leukocytes are displayed in the left panel. 48-hour ConA-activated C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553046/553047), FITC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553030/553031), and purified mAb UC10-4F10-11 (open histogram), or no purified antibody (filled histogram), in the presence of Mouse Fc Block™, followed by biotinylated anti-hamster IgG cocktail then Streptavidin-PE. The total viable T lymphocytes (CD4+ and/or CD8a+) are displayed on the right panel. Flow cytometry was performed on a BD FACScan™ System .

BD Pharmingen™ Purified Hamster Anti-Mouse CD152 - Purified - Clone UC10-4F10-11 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CTLA-4 IgG2a Fusion

Clone: UC10-4F10

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CTLA4 antibody (ABIN2688989)

Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, (2000) (PubMed).

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, (2000) (PubMed).

Issazadeh, Zhang, Sayegh, Khoury: "Acquired thymic tolerance: role of CTLA4 in the initiation and maintenance of tolerance in a clinically relevant autoimmune disease model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 2, pp. 761-5, (1999) (PubMed).

Lee, Chuang, Griffin, Khattri, Hong, Zhang, Straus, Samelson, Thompson, Bluestone: "Molecular basis of T cell inactivation by CTLA-4." in: Science (New York, N.Y.), Vol. 282, Issue 5397, pp. 2263-6, (1999) (PubMed).

Cilio, Daws, Malashicheva, Sentman, Holmberg: "Cytotoxic T lymphocyte antigen 4 is induced in the thymus upon in vivo activation and its blockade prevents anti-CD3-mediated depletion of thymocytes." in: The Journal of experimental medicine, Vol. 188, Issue 7, pp. 1239-46, (1998) (PubMed).

McCoy, Camberis, Gros: "Protective immunity to nematode infection is induced by CTLA-4 blockade." in: The Journal of experimental medicine, Vol. 186, Issue 2, pp. 183-7, (1997) (PubMed).

Tivol, Boyd, McKeon, Borriello, Nickerson, Strom, Sharpe: "CTLA4Ig prevents lymphoproliferation and fatal multiorgan tissue destruction in CTLA-4-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5091-4, (1997) (PubMed).

Waterhouse, Bachmann, Penninger, Ohashi, Mak: "Normal thymic selection, normal viability and decreased lymphoproliferation in T cell receptor-transgenic CTLA-4-deficient mice." in: European journal of immunology, Vol. 27, Issue 8, pp. 1887-92, (1997) (PubMed).

Alegre, Noel, Eisfelder, Chuang, Clark, Reiner, Thompson: "Regulation of surface and intracellular expression of CTLA4 on mouse T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 11, pp. 4762-70, (1996) (PubMed).

Linsley, Bradshaw, Greene, Peach, Bennett, Mittler: "Intracellular trafficking of CTLA-4 and focal localization towards sites of TCR engagement." in: Immunity, Vol. 4, Issue 6, pp. 535-43, (1996) (PubMed).

Marengère, Waterhouse, Duncan, Mittrücker, Feng, Mak: "Regulation of T cell receptor signaling by tyrosine phosphatase SYP association with CTLA-4." in: Science (New York, N.Y.), Vol. 272, Issue 5265, pp. 1170-3, (1996) (PubMed).

Perkins, Wang, Donovan, He, Mark, Guan, Wang, Walunas, Bluestone, Listman, Finn: "Regulation of CTLA-4 expression during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4154-9, (1996) (PubMed).

Kearney, Walunas, Karr, Morton, Loh, Bluestone, Jenkins: "Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1032-6, (1995) (PubMed).

Walunas, Lenschow, Bakker, Linsley, Freeman, Green, Thompson, Bluestone: "CTLA-4 can function as a negative regulator of T cell activation." in: Immunity, Vol. 1, Issue 5, pp. 405-13, (1995) (PubMed).

Waterhouse, Penninger, Timms, Wakeham, Shahinian, Lee, Thompson, Griesser, Mak: "Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4." in: Science (New York, N.Y.), Vol. 270, Issue 5238, pp. 985-8, (1995) (PubMed).

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, (1994) (PubMed).

Harper, Balzano, Rouvier, Mattéi, Luciani, Golstein: "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 3, pp. 1037-44, (1991) (PubMed).

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152 (CTLA4 Products)

Synonyms: CTLA-4 antibody, CTLA4 antibody, CD152 antibody, Cd152 antibody, Ctla-4 antibody, Ly-56 antibody, CD antibody, CELIAC3 antibody, GRD4 antibody, GSE antibody, IDDM12 antibody, sCTLA4 antibody, cytotoxic T-lymphocyte associated protein 4 antibody, cytotoxic T-lymphocyte-associated protein 4 antibody, CTLA4 antibody, Ctla4 antibody

Background: Synonyms: CTLA-4

Pathways: Cancer Immune Checkpoints