CD40 Ligand antibody

Catalog No. ABIN2688992

Product Details anti-CD40 Ligand Antibody

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Rhesus Monkey, Baboon, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Reacts with CD40 ligand (CD40L), a 39 kDa type II membrane glycoprotein expressed on activated T cells. CD40-CD40L interaction plays a very important role in T cell-dependent B-cell proliferation, differentiation, and memory cell formation. Anti-CD40L mAb partially blocks T cell-B cell interaction affecting subsequent proliferation, differentiation, and memory cell formation. Anti-CD40L mAb partially blocks T cell-B cell interaction affecting subsequent proliferation, IL-2R expression and differentiation of B cells. In addition, blocking of CD40-CD40L interaction has been demonstrated with soluble CD40, resulting in the inhibition of immunoglobulin isotype switching. It has been reported that patients with X-linked hyper-IgM syndrome have defective expression of functional CD40L due to a defective gene that encodes CD40L. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of TPA+Ca++ Ionophore-stimulated PBMCs analyzed on a FACScan

BD Pharmingen™ Purified Mouse Anti-Human CD154 - Purified - Clone TRAP1 (also known as TRAP-1) - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Rhe - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: TRAP1

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD40 Ligand antibody (ABIN2688992)

Fuleihan, Ramesh, Horner, Ahern, Belshaw, Alberg, Stamenkovic, Harmon, Geha: "Cyclosporin A inhibits CD40 ligand expression in T lymphocytes." in: The Journal of clinical investigation, Vol. 93, Issue 3, pp. 1315-20, (1994) (PubMed).

Gray, Dullforce, Jainandunsing: "Memory B cell development but not germinal center formation is impaired by in vivo blockade of CD40-CD40 ligand interaction." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 141-55, (1994) (PubMed).

Nishioka, Lipsky: "The role of CD40-CD40 ligand interaction in human T cell-B cell collaboration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1027-36, (1994) (PubMed).

van Kooten, Gaillard, Galizzi, Hermann, Fossiez, Banchereau, Blanchard: "B cells regulate expression of CD40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD40." in: European journal of immunology, Vol. 24, Issue 4, pp. 787-92, (1994) (PubMed).

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 (CD40LG Products)

Synonyms: CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody

Background: Synonyms: CD40L

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints