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SIRPA antibody

SIRPA Reactivity: Mouse WB, FACS, IP, IF, BR Host: Rat Monoclonal P84 unconjugated
Catalog No. ABIN2689017
  • Target See all SIRPA Antibodies
    SIRPA (Signal-Regulatory Protein alpha (SIRPA))
    Reactivity
    • 72
    • 39
    • 33
    • 10
    • 8
    • 4
    • 3
    • 3
    Mouse
    Host
    • 59
    • 32
    • 1
    Rat
    Clonality
    • 56
    • 36
    Monoclonal
    Conjugate
    • 46
    • 11
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SIRPA antibody is un-conjugated
    Application
    • 57
    • 46
    • 19
    • 19
    • 13
    • 13
    • 11
    • 10
    • 9
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Blocking Reagent (BR)
    Brand
    BD Pharmingen™
    Characteristics
    The P84 antibody reacts with CD172a, also known as SIgnal-Regulatory Protein α (SIRPα), Src Homology 2 domain-containing protein tyrosine Phosphatase (SHP) Substrate 1 (SHPS-1), or Brain Immunoglobulin-like molecule with Tyrosine-based activation motifs (BIT). CD172a is an adhesion molecule of the Ig superfamily which is expressed on neurons in the central nervous system and the retina, on macrophages, and on bone-marrow myeloid cells. Its ligand, CD47, or Integrin-Associated Protein (IAP), is expressed by a wide variety of cells. CD172a and CD47 are proposed to mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. Two-color analysis of CD172a expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified rat IgG1 isotype control mAb (left panel) or purified mAb P84 (right panel), followed by FITC-conjugated anti-rat IgG1 mAb RG11/39.4 (Cat. No. 553892, both panels), then PE-conjugated anti-mouse CD11b (Mac-1) mAb M1/70 (Cat. No. 557397/553311). Total viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibura .

    BD Pharmingen™ Purified Rat Anti-Mouse CD172a - Purified - Clone P84 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Membrane fractions from mouse brain
    Clone
    P84
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product SIRPA Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Mi, Jiang, Weng, Lindberg, Narayanan, Lagenaur: "Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina." in: The Journal of comparative neurology, Vol. 416, Issue 3, pp. 335-44, (2000) (PubMed).

    Gresham, Dale, Potter, Chang, Vines, Lowell, Lagenaur, Willman: "Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr." in: The Journal of experimental medicine, Vol. 191, Issue 3, pp. 515-28, (2000) (PubMed).

    Oldenborg, Zheleznyak, Fang, Lagenaur, Gresham, Lindberg: "Role of CD47 as a marker of self on red blood cells." in: Science (New York, N.Y.), Vol. 288, Issue 5473, pp. 2051-4, (2000) (PubMed).

    Jiang, Lagenaur, Narayanan: "Integrin-associated protein is a ligand for the P84 neural adhesion molecule." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 559-62, (1999) (PubMed).

    Veillette, Thibaudeau, Latour: "High expression of inhibitory receptor SHPS-1 and its association with protein-tyrosine phosphatase SHP-1 in macrophages." in: The Journal of biological chemistry, Vol. 273, Issue 35, pp. 22719-28, (1998) (PubMed).

    Comu, Weng, Olinsky, Ishwad, Mi, Hempel, Watkins, Lagenaur, Narayanan: "The murine P84 neural adhesion molecule is SHPS-1, a member of the phosphatase-binding protein family." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 22, pp. 8702-10, (1997) (PubMed).

    Chuang, Lagenaur: "Central nervous system antigen P84 can serve as a substrate for neurite outgrowth." in: Developmental biology, Vol. 137, Issue 2, pp. 219-32, (1990) (PubMed).

  • Target
    SIRPA (Signal-Regulatory Protein alpha (SIRPA))
    Alternative Name
    CD172a (SIRPA Products)
    Synonyms
    BIT antibody, CD172A antibody, MFR antibody, MYD-1 antibody, P84 antibody, PTPNS1 antibody, SHPS1 antibody, SIRP antibody, SIRPA antibody, SIRP-A1 antibody, SIRP-B1 antibody, AI835480 antibody, Bit antibody, CD172a antibody, Ptpns1 antibody, SHP-1 antibody, SHPS-1 antibody, swc3 antibody, signal regulatory protein alpha antibody, myeloperoxidase antibody, signal-regulatory protein alpha antibody, tyrosine-protein phosphatase non-receptor type substrate 1 antibody, SIRPA antibody, MPO antibody, LOC458039 antibody, LOC100400378 antibody, LOC100590224 antibody, Sirpa antibody
    Background
    Synonyms: SIRPα, SHPS-1, BIT
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