CD180 antibody

Catalog No. ABIN2689036

Product Details anti-CD180 Antibody

Target: CD180 (CD180 Molecule (CD180))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD180 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Reacts with RP105/Bgp95, a 95-105 kDa type I membrane protein consisting of extracellular leucine-rich repeats and a short cytoplasmic domain. It is expressed on mantle zone B cells, but weakly on germinal center B cells. RP105/Bgp95 is also expressed on peripheral blood monocytes, dendritic cells, and a subset of peripheral blood lymphocytes. The extracellular domain associates with a molecule called MD-1 to form a cell surface receptor complex RP105/Bgp95/MD- 1. This receptor belongs to the family of toll-like receptors (TLR). Studies show that RP105/Bgp95/MD-1, working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS). Reports on functional studies show that G28-8 monoclonal antibody can induce a G0 to G1 cell cycle transition and was synergistic with PMA, anti-μ, or anti-CD40 in inducing proliferation of resting B cells. Profile of peripheral blood lymphocytes analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human CD180 - Purified - Clone G28-8 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: G28

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD180 antibody (ABIN2689036)

Ogata, Su, Miyake, Nagai, Akashi, Mecklenbräuker, Rajewsky, Kimoto, Tarakhovsky: "The toll-like receptor protein RP105 regulates lipopolysaccharide signaling in B cells." in: The Journal of experimental medicine, Vol. 192, Issue 1, pp. 23-9, (2000) (PubMed).

Roshak, Anderson, Holmes, Jonak, Bolognese, Terrett, Marshall: "Anti-human RP105 sera induces lymphocyte proliferation." in: Journal of leukocyte biology, Vol. 65, Issue 1, pp. 43-9, (1999) (PubMed).

Fugier-Vivier, de Bouteiller, Guret, Fossiez, Banchereau, Mattei, Aït-Yahia, Garcia, Lebecque, Liu: "Molecular cloning of human RP105." in: European journal of immunology, Vol. 27, Issue 7, pp. 1824-7, (1997) (PubMed).

Valentine, Clark, Shu, Norris, Ledbetter: "Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4071-8, (1988) (PubMed).

Target Details for CD180

Target: CD180 (CD180 Molecule (CD180))

Alternative Name: CD180 (CD180 Products)

Synonyms: Ly78 antibody, CD180 antibody, LY64 antibody, RP105 antibody, F630107B15 antibody, CD180 molecule antibody, CD180 antigen antibody, Cd180 antibody, CD180 antibody

Background: Synonyms: RP105

Pathways: Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades